Biotransformation Of Ferulic Acid Extracted From Biomass To Biovanillin By Amycolatopsis Sp. Atcc 39116

This study focuses on the biotransformation of synthetic and extracted ferulic acid (FA) from lignocellulosic biomass using growing cells of Amyco/alopsis sp. ATCC 39116 as a biocatalyst. Preliminary alkaline hydrolysis showed that the hydrolyzate from oil palm empty fruit bunch (OPEFB) contained...

Full description

Saved in:

Bibliographic Details
Main Author:	Abu Sepian, Noor Raihana
Format:	Thesis
Language:	English
Published:	2014
Subjects:	TP1-1185 Chemical technology
Online Access:	http://eprints.usm.my/60852/1/24%20Pages%20from%2000001785213.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.60852
record_format	uketd_dc
spelling	my-usm-ep.608522024-07-18T02:15:33Z Biotransformation Of Ferulic Acid Extracted From Biomass To Biovanillin By Amycolatopsis Sp. Atcc 39116 2014-11 Abu Sepian, Noor Raihana TP1-1185 Chemical technology This study focuses on the biotransformation of synthetic and extracted ferulic acid (FA) from lignocellulosic biomass using growing cells of Amyco/alopsis sp. ATCC 39116 as a biocatalyst. Preliminary alkaline hydrolysis showed that the hydrolyzate from oil palm empty fruit bunch (OPEFB) contained the highest amount of FA (199.4 mg/l) among the other three lignocellulosic wastes (i.e. oil palm frond, coconut fiber and sheIl). Optimization of extraction was carried out using Response Surface Methodology (RSM) via Central Composite Design (CCD) on the effect of three independent variables, i.e. concentration of NaOH (1.5-5 M), solid/liquid ratio (0.03-0.084 g/ml) and time (16-32 h). The optimum FA concentration of 2.04 mg/g OPEFB (365.19 mg/l) was obtained under the optimal conditions of 1.5 M ofNaOH concentration, 0.03 g/ml of solid/liquid ratio and 32 h of extraction time. The extracted FA was further purified using polyvinylpolypyrrolidone (PVPP) and activated carbon, and about 97.26% was successfully recovered from the extract using PVPP. The partially purified FA was converted into 38.47% molar yield of vanillin, showing the ability of Amyco/alopsis sp. ATCC 39116 to convert the extracted FA from lignocellulosic waste. 2014-11 Thesis http://eprints.usm.my/60852/ http://eprints.usm.my/60852/1/24%20Pages%20from%2000001785213.pdf application/pdf en public masters Perpustakaan Hamzah Sendut Pusat Pengajian Kejuruteraan Kimia
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	TP1-1185 Chemical technology
spellingShingle	TP1-1185 Chemical technology Abu Sepian, Noor Raihana Biotransformation Of Ferulic Acid Extracted From Biomass To Biovanillin By Amycolatopsis Sp. Atcc 39116
description	This study focuses on the biotransformation of synthetic and extracted ferulic acid (FA) from lignocellulosic biomass using growing cells of Amyco/alopsis sp. ATCC 39116 as a biocatalyst. Preliminary alkaline hydrolysis showed that the hydrolyzate from oil palm empty fruit bunch (OPEFB) contained the highest amount of FA (199.4 mg/l) among the other three lignocellulosic wastes (i.e. oil palm frond, coconut fiber and sheIl). Optimization of extraction was carried out using Response Surface Methodology (RSM) via Central Composite Design (CCD) on the effect of three independent variables, i.e. concentration of NaOH (1.5-5 M), solid/liquid ratio (0.03-0.084 g/ml) and time (16-32 h). The optimum FA concentration of 2.04 mg/g OPEFB (365.19 mg/l) was obtained under the optimal conditions of 1.5 M ofNaOH concentration, 0.03 g/ml of solid/liquid ratio and 32 h of extraction time. The extracted FA was further purified using polyvinylpolypyrrolidone (PVPP) and activated carbon, and about 97.26% was successfully recovered from the extract using PVPP. The partially purified FA was converted into 38.47% molar yield of vanillin, showing the ability of Amyco/alopsis sp. ATCC 39116 to convert the extracted FA from lignocellulosic waste.
format	Thesis
qualification_level	Master's degree
author	Abu Sepian, Noor Raihana
author_facet	Abu Sepian, Noor Raihana
author_sort	Abu Sepian, Noor Raihana
title	Biotransformation Of Ferulic Acid Extracted From Biomass To Biovanillin By Amycolatopsis Sp. Atcc 39116
title_short	Biotransformation Of Ferulic Acid Extracted From Biomass To Biovanillin By Amycolatopsis Sp. Atcc 39116
title_full	Biotransformation Of Ferulic Acid Extracted From Biomass To Biovanillin By Amycolatopsis Sp. Atcc 39116
title_fullStr	Biotransformation Of Ferulic Acid Extracted From Biomass To Biovanillin By Amycolatopsis Sp. Atcc 39116
title_full_unstemmed	Biotransformation Of Ferulic Acid Extracted From Biomass To Biovanillin By Amycolatopsis Sp. Atcc 39116
title_sort	biotransformation of ferulic acid extracted from biomass to biovanillin by amycolatopsis sp. atcc 39116
granting_institution	Perpustakaan Hamzah Sendut
granting_department	Pusat Pengajian Kejuruteraan Kimia
publishDate	2014
url	http://eprints.usm.my/60852/1/24%20Pages%20from%2000001785213.pdf
_version_	1804889008031924224

Biotransformation Of Ferulic Acid Extracted From Biomass To Biovanillin By Amycolatopsis Sp. Atcc 39116

Similar Items