Expression of sema4d and its receptor Plexin-b1 in invasive breast ductal Carcinoma in relation to Tumor angiogenesis and Tumor-associated macrophages

Cumulative experimental and clinical studies have shown involvement of Sema4D, a Class IV semaphorin, and its receptor, Plexin-Bl in tumor progression. Particularly, regulation of monocytic cells by Sema4D, and tumor angiogenesis via Sema4D/Plexin-B 1 receptor system are of great interest. This s...

Full description

Saved in:

Bibliographic Details
Main Author:	Seng,, Ch’ng Ewe
Format:	Thesis
Language:	English
Published:	2012
Subjects:	R Medicine (General)
Online Access:	http://eprints.usm.my/60936/1/CHING%20EWE%20SENG%20-%20e.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.60936
record_format	uketd_dc
spelling	my-usm-ep.609362024-11-17T06:46:28Z Expression of sema4d and its receptor Plexin-b1 in invasive breast ductal Carcinoma in relation to Tumor angiogenesis and Tumor-associated macrophages 2012 Seng,, Ch’ng Ewe R Medicine (General) RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Cumulative experimental and clinical studies have shown involvement of Sema4D, a Class IV semaphorin, and its receptor, Plexin-Bl in tumor progression. Particularly, regulation of monocytic cells by Sema4D, and tumor angiogenesis via Sema4D/Plexin-B 1 receptor system are of great interest. This study characterized the expressions of Sema4D and Plexin-B 1 in human invasive breast ductal carcinoma and evaluated their relationships with other pertinent clinicopathological parameters in general, and with tumor-associated macrophages and tumor angiogenesis in particular. Expressions of Sema4D and Plexin-Bl in 94 patients diagnosed of invasive ductal carcinoma, NOS were explored immunohistochemically on paraffinembedded tissue sections. For each section, three best-stained hotspots and the wholeslide tumor area were evaluated using an intensity distribution score (IDS), a modified H-score system. In addition, tumor-associated macrophages highlighted by anti-CD68 antibody were evaluated with reference to their histological locations whether in the tumor nests or the tumor stroma. Microvessels were immunostained with anti-CD34 antibody and counted for microvessel density. Invasive ductal carcinomas variably expressed Sema4D and Plexin-Bl. Their expressions showed weak significant correlation when the whole-slide IDSs were evaluated (r=0.208, p=0.045). The average 3-hotspot IDS of Sema4D expression showed positive association with Her-2 expression (p=0.032), while the whole-slide IDS was associated with positive hormonal receptor status (p-0.022). High average 3- hotspot IDS of Plexin-Bl expression had higher numbers of lymph node metastasis (p=0.032). Limiting to estrogen receptor positive cases or Her-2 overexpressed cases, high Plexin-Bl expression by the whole-slide assessment was paradoxically associated with absence of lymph node metastasis (p=0.009 and p=0.039, respectively). In relation to tumor-associated macrophages, higher levels of Sema4D expression by the wholeslide assessment were observed in lower grades of tumor stromal macrophages (p=0.001), but no such relationship was observed for Plexin-Bl. Both Sema4D and Plexin-Bl expressions had no relationship with the tumor nest macrophage counts. With regard to tumor angiogenesis, Plexin-Bl expression assessed by 3-hotspot methodology demonstrated weak positive correlation with microvessel density (r=0.206, p=0.047). Sema4D expression was not correlated to microvessel density. Heterogeneity of Sema4D and Plexin-Bl expressions in human invasive breast ductal carcinoma was demonstrated. Their expressions were associated with a few traditional predictive and prognostic factors. Sema4D expressed in tumors appeared to have an inhibitory effect on the tumor stromal macrophages. In contrast to experimental studies, proangiogenic properties of Sema4D could not be validated. Focal expression of Plexin-Bl might be a weak marker for a more angiogenic tumor. However, Plexin-Bl expression was not associated with tumor-associated macrophages. 2012 Thesis http://eprints.usm.my/60936/ http://eprints.usm.my/60936/1/CHING%20EWE%20SENG%20-%20e.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine (General) R Medicine (General)
spellingShingle	R Medicine (General) R Medicine (General) Seng,, Ch’ng Ewe Expression of sema4d and its receptor Plexin-b1 in invasive breast ductal Carcinoma in relation to Tumor angiogenesis and Tumor-associated macrophages
description	Cumulative experimental and clinical studies have shown involvement of Sema4D, a Class IV semaphorin, and its receptor, Plexin-Bl in tumor progression. Particularly, regulation of monocytic cells by Sema4D, and tumor angiogenesis via Sema4D/Plexin-B 1 receptor system are of great interest. This study characterized the expressions of Sema4D and Plexin-B 1 in human invasive breast ductal carcinoma and evaluated their relationships with other pertinent clinicopathological parameters in general, and with tumor-associated macrophages and tumor angiogenesis in particular. Expressions of Sema4D and Plexin-Bl in 94 patients diagnosed of invasive ductal carcinoma, NOS were explored immunohistochemically on paraffinembedded tissue sections. For each section, three best-stained hotspots and the wholeslide tumor area were evaluated using an intensity distribution score (IDS), a modified H-score system. In addition, tumor-associated macrophages highlighted by anti-CD68 antibody were evaluated with reference to their histological locations whether in the tumor nests or the tumor stroma. Microvessels were immunostained with anti-CD34 antibody and counted for microvessel density. Invasive ductal carcinomas variably expressed Sema4D and Plexin-Bl. Their expressions showed weak significant correlation when the whole-slide IDSs were evaluated (r=0.208, p=0.045). The average 3-hotspot IDS of Sema4D expression showed positive association with Her-2 expression (p=0.032), while the whole-slide IDS was associated with positive hormonal receptor status (p-0.022). High average 3- hotspot IDS of Plexin-Bl expression had higher numbers of lymph node metastasis (p=0.032). Limiting to estrogen receptor positive cases or Her-2 overexpressed cases, high Plexin-Bl expression by the whole-slide assessment was paradoxically associated with absence of lymph node metastasis (p=0.009 and p=0.039, respectively). In relation to tumor-associated macrophages, higher levels of Sema4D expression by the wholeslide assessment were observed in lower grades of tumor stromal macrophages (p=0.001), but no such relationship was observed for Plexin-Bl. Both Sema4D and Plexin-Bl expressions had no relationship with the tumor nest macrophage counts. With regard to tumor angiogenesis, Plexin-Bl expression assessed by 3-hotspot methodology demonstrated weak positive correlation with microvessel density (r=0.206, p=0.047). Sema4D expression was not correlated to microvessel density. Heterogeneity of Sema4D and Plexin-Bl expressions in human invasive breast ductal carcinoma was demonstrated. Their expressions were associated with a few traditional predictive and prognostic factors. Sema4D expressed in tumors appeared to have an inhibitory effect on the tumor stromal macrophages. In contrast to experimental studies, proangiogenic properties of Sema4D could not be validated. Focal expression of Plexin-Bl might be a weak marker for a more angiogenic tumor. However, Plexin-Bl expression was not associated with tumor-associated macrophages.
format	Thesis
qualification_level	Master's degree
author	Seng,, Ch’ng Ewe
author_facet	Seng,, Ch’ng Ewe
author_sort	Seng,, Ch’ng Ewe
title	Expression of sema4d and its receptor Plexin-b1 in invasive breast ductal Carcinoma in relation to Tumor angiogenesis and Tumor-associated macrophages
title_short	Expression of sema4d and its receptor Plexin-b1 in invasive breast ductal Carcinoma in relation to Tumor angiogenesis and Tumor-associated macrophages
title_full	Expression of sema4d and its receptor Plexin-b1 in invasive breast ductal Carcinoma in relation to Tumor angiogenesis and Tumor-associated macrophages
title_fullStr	Expression of sema4d and its receptor Plexin-b1 in invasive breast ductal Carcinoma in relation to Tumor angiogenesis and Tumor-associated macrophages
title_full_unstemmed	Expression of sema4d and its receptor Plexin-b1 in invasive breast ductal Carcinoma in relation to Tumor angiogenesis and Tumor-associated macrophages
title_sort	expression of sema4d and its receptor plexin-b1 in invasive breast ductal carcinoma in relation to tumor angiogenesis and tumor-associated macrophages
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2012
url	http://eprints.usm.my/60936/1/CHING%20EWE%20SENG%20-%20e.pdf
_version_	1818647359654985728

Expression of sema4d and its receptor Plexin-b1 in invasive breast ductal Carcinoma in relation to Tumor angiogenesis and Tumor-associated macrophages

Similar Items