Establishment Of The Callus And Cell Suspension Cultures For The Growth Of Pogostemon Cablin

In this study, plant cell culture technique was used in order to investigate the production of patchouli alcohol in callus and cell suspension cultures. The callus cultures were induced from the intact Pogostemon cablin leaves called explants. Different types, concentration of plant growth regula...

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Main Author:	Che Saad, Norlia
Format:	Thesis
Language:	English
Published:	2014
Subjects:	TP1-1185 Chemical technology
Online Access:	http://eprints.usm.my/61126/1/24%20Pages%20from%2000001785140.pdf
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id	my-usm-ep.61126
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spelling	my-usm-ep.611262024-09-17T02:39:22Z Establishment Of The Callus And Cell Suspension Cultures For The Growth Of Pogostemon Cablin 2014-12 Che Saad, Norlia TP1-1185 Chemical technology In this study, plant cell culture technique was used in order to investigate the production of patchouli alcohol in callus and cell suspension cultures. The callus cultures were induced from the intact Pogostemon cablin leaves called explants. Different types, concentration of plant growth regulators (PGRs) and different culture conditions (light, carbon source and inoculum size) were studied in order to produce a rapid-growing callus cultures and well-dispersed cell suspension cultures or P. cublin. The best condition for callus cultures growth was obtained in the Murashige and Skoog (MS) basal medium containing I mg/L picloram (MS I d) which was maintained in the dark for 21 days. Callus cultures were healthy with whitish and friable features. However, the cultured explants showed negative response to MS medium supplemented with 2, 4- D and all combination of picloram and 2, 4-0. The healthy, fast growing and friable callus cultures were then used to establish the cell suspension cultures. Under the influences of different concentrations and combinations of PGRs and light, rapid growth of cell suspension cultures were observed in the MDI medium. 2014-12 Thesis http://eprints.usm.my/61126/ http://eprints.usm.my/61126/1/24%20Pages%20from%2000001785140.pdf application/pdf en public masters Perpustakaan Hamzah Sendut Pusat Pengajian Kejuruteraan Kimia
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	TP1-1185 Chemical technology
spellingShingle	TP1-1185 Chemical technology Che Saad, Norlia Establishment Of The Callus And Cell Suspension Cultures For The Growth Of Pogostemon Cablin
description	In this study, plant cell culture technique was used in order to investigate the production of patchouli alcohol in callus and cell suspension cultures. The callus cultures were induced from the intact Pogostemon cablin leaves called explants. Different types, concentration of plant growth regulators (PGRs) and different culture conditions (light, carbon source and inoculum size) were studied in order to produce a rapid-growing callus cultures and well-dispersed cell suspension cultures or P. cublin. The best condition for callus cultures growth was obtained in the Murashige and Skoog (MS) basal medium containing I mg/L picloram (MS I d) which was maintained in the dark for 21 days. Callus cultures were healthy with whitish and friable features. However, the cultured explants showed negative response to MS medium supplemented with 2, 4- D and all combination of picloram and 2, 4-0. The healthy, fast growing and friable callus cultures were then used to establish the cell suspension cultures. Under the influences of different concentrations and combinations of PGRs and light, rapid growth of cell suspension cultures were observed in the MDI medium.
format	Thesis
qualification_level	Master's degree
author	Che Saad, Norlia
author_facet	Che Saad, Norlia
author_sort	Che Saad, Norlia
title	Establishment Of The Callus And Cell Suspension Cultures For The Growth Of Pogostemon Cablin
title_short	Establishment Of The Callus And Cell Suspension Cultures For The Growth Of Pogostemon Cablin
title_full	Establishment Of The Callus And Cell Suspension Cultures For The Growth Of Pogostemon Cablin
title_fullStr	Establishment Of The Callus And Cell Suspension Cultures For The Growth Of Pogostemon Cablin
title_full_unstemmed	Establishment Of The Callus And Cell Suspension Cultures For The Growth Of Pogostemon Cablin
title_sort	establishment of the callus and cell suspension cultures for the growth of pogostemon cablin
granting_institution	Perpustakaan Hamzah Sendut
granting_department	Pusat Pengajian Kejuruteraan Kimia
publishDate	2014
url	http://eprints.usm.my/61126/1/24%20Pages%20from%2000001785140.pdf
_version_	1811772882496258048

Establishment Of The Callus And Cell Suspension Cultures For The Growth Of Pogostemon Cablin

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