Metabolomics Analysis Of Blood And Urine To Identify Alcohol-Dependence Biomarkers
Alcohol misuse is a ravaging public health and social problem. Alcoholdependence (AD) is a phase of alcohol misuse in which the drinker consumes excessive amount of alcohol and have a continuous urge to consume alcohol. The current methods of alcohol dependence diagnosis are questionnaires and so...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
2015
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/61164/1/24%20Pages%20from%2000001792372.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
my-usm-ep.61164 |
|---|---|
| record_format |
uketd_dc |
| spelling |
my-usm-ep.611642024-09-19T04:05:05Z Metabolomics Analysis Of Blood And Urine To Identify Alcohol-Dependence Biomarkers 2015-07 Mostafa, Hamza Mohamed Amin RS1-441 Pharmacy and materia medica Alcohol misuse is a ravaging public health and social problem. Alcoholdependence (AD) is a phase of alcohol misuse in which the drinker consumes excessive amount of alcohol and have a continuous urge to consume alcohol. The current methods of alcohol dependence diagnosis are questionnaires and some biomarkers. However, both methods lack specificity and sensitivity. Metabolomics is a novel scientific field which may provide a novel method for the diagnosis of alcohol-dependence by using a sensitive and specific technique such as nuclear magnetic resonance (NMR). Our aim was to identify the metabolic fingerprint (metabotype) that is able to discriminate between AD, non-AD alcohol drinkers and controls using metabolomics approach. The reproducibility of the outcomes was also investigated. Blood and urine samples were collected from 30 alcohol-dependent (mean age: 45.7), 54 social drinkers (mean age: 39.5) and 60 controls (mean age: 37.1). Plasma was separated by centrifugation, then both plasma and urine samples were mixed with phosphate buffer which was prepared by dissolving di-sodium hydrogen phosphate (Na2HP04), sodium dihydrogen phosphate (NaH2P04), sodium 3-(trimethylsilyl)-propionate-2,2,3,3- 2015-07 Thesis http://eprints.usm.my/61164/ http://eprints.usm.my/61164/1/24%20Pages%20from%2000001792372.pdf application/pdf en public masters Perpustakaan Hamzah Sendut Pusat Pengajian Sains Farmasi |
| institution |
Universiti Sains Malaysia |
| collection |
USM Institutional Repository |
| language |
English |
| topic |
RS1-441 Pharmacy and materia medica |
| spellingShingle |
RS1-441 Pharmacy and materia medica Mostafa, Hamza Mohamed Amin Metabolomics Analysis Of Blood And Urine To Identify Alcohol-Dependence Biomarkers |
| description |
Alcohol misuse is a ravaging public health and social problem. Alcoholdependence
(AD) is a phase of alcohol misuse in which the drinker consumes
excessive amount of alcohol and have a continuous urge to consume alcohol. The
current methods of alcohol dependence diagnosis are questionnaires and some
biomarkers. However, both methods lack specificity and sensitivity. Metabolomics is
a novel scientific field which may provide a novel method for the diagnosis of
alcohol-dependence by using a sensitive and specific technique such as nuclear
magnetic resonance (NMR). Our aim was to identify the metabolic fingerprint
(metabotype) that is able to discriminate between AD, non-AD alcohol drinkers and
controls using metabolomics approach. The reproducibility of the outcomes was also
investigated. Blood and urine samples were collected from 30 alcohol-dependent
(mean age: 45.7), 54 social drinkers (mean age: 39.5) and 60 controls (mean age:
37.1). Plasma was separated by centrifugation, then both plasma and urine samples
were mixed with phosphate buffer which was prepared by dissolving di-sodium
hydrogen phosphate (Na2HP04), sodium dihydrogen phosphate (NaH2P04), sodium
3-(trimethylsilyl)-propionate-2,2,3,3- |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Mostafa, Hamza Mohamed Amin |
| author_facet |
Mostafa, Hamza Mohamed Amin |
| author_sort |
Mostafa, Hamza Mohamed Amin |
| title |
Metabolomics Analysis Of Blood And Urine To Identify Alcohol-Dependence Biomarkers |
| title_short |
Metabolomics Analysis Of Blood And Urine To Identify Alcohol-Dependence Biomarkers |
| title_full |
Metabolomics Analysis Of Blood And Urine To Identify Alcohol-Dependence Biomarkers |
| title_fullStr |
Metabolomics Analysis Of Blood And Urine To Identify Alcohol-Dependence Biomarkers |
| title_full_unstemmed |
Metabolomics Analysis Of Blood And Urine To Identify Alcohol-Dependence Biomarkers |
| title_sort |
metabolomics analysis of blood and urine to identify alcohol-dependence biomarkers |
| granting_institution |
Perpustakaan Hamzah Sendut |
| granting_department |
Pusat Pengajian Sains Farmasi |
| publishDate |
2015 |
| url |
http://eprints.usm.my/61164/1/24%20Pages%20from%2000001792372.pdf |
| _version_ |
1811772888257134592 |