Production Of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) By Cupriavidus Malaysiensis Usmaa2-4Abh16 Harbouring Cupriavidus Necator H16 Lipase Gene

Polyhydroxyalkanoates (PHA) are biopolyesters with thermoplastic properties that are biocompatible and biodegradable. Poly(3-hydroxybutyrate) [P(3HB)] is the most common PHA produced by various microorganisms, but its application is limited due to its stiffness. Incorporating a secondary monomer suc...

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Main Author: Jeremy, Wong Hau Seung
Format: Thesis
Language:English
Published: 2023
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Online Access:http://eprints.usm.my/61275/1/24%20Pages%20from%20JEREMY%20WONG%20HAU%20SEUNG.pdf
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spelling my-usm-ep.612752024-10-14T01:18:33Z Production Of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) By Cupriavidus Malaysiensis Usmaa2-4Abh16 Harbouring Cupriavidus Necator H16 Lipase Gene 2023-02 Jeremy, Wong Hau Seung QD1-999 Chemistry Polyhydroxyalkanoates (PHA) are biopolyesters with thermoplastic properties that are biocompatible and biodegradable. Poly(3-hydroxybutyrate) [P(3HB)] is the most common PHA produced by various microorganisms, but its application is limited due to its stiffness. Incorporating a secondary monomer such as 3-hydroxyvalerate (3HV) contributes to several PHA properties improvement, including flexibility, elasticity, and biodegradability. High-cost oleic acid and organic acids are preferred by most PHA-producing bacteria as the carbon sources for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] production. This study demonstrated the evaluation of a lipase genes-harbouring transformant for PHA production from palm olein and 1-pentanol as alternatives to oleic acid and valeric acid. In this study, a clone, Cupriavidus malaysiensis USMAA2-4ABH16 which is the transformant of C. malaysiensis USMAA2-4 was successfully constructed. C. malaysiensis USMAA2-4ABH16 harbouring expressible Cupriavidus necator H16 lipAB genes accumulated more than 60 wt% of P(3HB-co-3HV) using palm olein and 1-pentanol as the carbon sources. Nucleotide sequence alignment of the lipAB insert showed 100% identities with C. necator H16 lipAB genes and expect value of 0.0. C. malaysiensis USMAA2-4ABH16 showed 4-fold higher growth and 8-fold higher lipase activity compared to C. necator H16 under cobalt and alcohol stress. 2023-02 Thesis http://eprints.usm.my/61275/ http://eprints.usm.my/61275/1/24%20Pages%20from%20JEREMY%20WONG%20HAU%20SEUNG.pdf application/pdf en public phd doctoral Perpustakaan Hamzah Sendut Pusat Pengajian Sains Kimia
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic QD1-999 Chemistry
spellingShingle QD1-999 Chemistry
Jeremy, Wong Hau Seung
Production Of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) By Cupriavidus Malaysiensis Usmaa2-4Abh16 Harbouring Cupriavidus Necator H16 Lipase Gene
description Polyhydroxyalkanoates (PHA) are biopolyesters with thermoplastic properties that are biocompatible and biodegradable. Poly(3-hydroxybutyrate) [P(3HB)] is the most common PHA produced by various microorganisms, but its application is limited due to its stiffness. Incorporating a secondary monomer such as 3-hydroxyvalerate (3HV) contributes to several PHA properties improvement, including flexibility, elasticity, and biodegradability. High-cost oleic acid and organic acids are preferred by most PHA-producing bacteria as the carbon sources for poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] production. This study demonstrated the evaluation of a lipase genes-harbouring transformant for PHA production from palm olein and 1-pentanol as alternatives to oleic acid and valeric acid. In this study, a clone, Cupriavidus malaysiensis USMAA2-4ABH16 which is the transformant of C. malaysiensis USMAA2-4 was successfully constructed. C. malaysiensis USMAA2-4ABH16 harbouring expressible Cupriavidus necator H16 lipAB genes accumulated more than 60 wt% of P(3HB-co-3HV) using palm olein and 1-pentanol as the carbon sources. Nucleotide sequence alignment of the lipAB insert showed 100% identities with C. necator H16 lipAB genes and expect value of 0.0. C. malaysiensis USMAA2-4ABH16 showed 4-fold higher growth and 8-fold higher lipase activity compared to C. necator H16 under cobalt and alcohol stress.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Jeremy, Wong Hau Seung
author_facet Jeremy, Wong Hau Seung
author_sort Jeremy, Wong Hau Seung
title Production Of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) By Cupriavidus Malaysiensis Usmaa2-4Abh16 Harbouring Cupriavidus Necator H16 Lipase Gene
title_short Production Of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) By Cupriavidus Malaysiensis Usmaa2-4Abh16 Harbouring Cupriavidus Necator H16 Lipase Gene
title_full Production Of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) By Cupriavidus Malaysiensis Usmaa2-4Abh16 Harbouring Cupriavidus Necator H16 Lipase Gene
title_fullStr Production Of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) By Cupriavidus Malaysiensis Usmaa2-4Abh16 Harbouring Cupriavidus Necator H16 Lipase Gene
title_full_unstemmed Production Of Poly(3-Hydroxybutyrate-Co-3-Hydroxyvalerate) By Cupriavidus Malaysiensis Usmaa2-4Abh16 Harbouring Cupriavidus Necator H16 Lipase Gene
title_sort production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by cupriavidus malaysiensis usmaa2-4abh16 harbouring cupriavidus necator h16 lipase gene
granting_institution Perpustakaan Hamzah Sendut
granting_department Pusat Pengajian Sains Kimia
publishDate 2023
url http://eprints.usm.my/61275/1/24%20Pages%20from%20JEREMY%20WONG%20HAU%20SEUNG.pdf
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