Siam weed based hydrogel hybrid for tissue engineering
Tissue engineered skin grafts have been considered as an auxiliary for replacement of damaged skin due to burns and injury. In previous studies, gelatin hybrid with natural remedy were used in order to create 3D environment for the skin grafts. However, these gelatin hybrids were not sufficien...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English English English |
| Published: |
2020
|
| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/1052/1/24p%20NOR%20SHAMSIAH%20MOHAMED.pdf http://eprints.uthm.edu.my/1052/2/NOR%20SHAMSIAH%20MOHAMED%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/1052/3/NOR%20SHAMSIAH%20MOHAMED%20WATERMARK.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | Tissue engineered skin grafts have been considered as an auxiliary for replacement of
damaged skin due to burns and injury. In previous studies, gelatin hybrid with natural
remedy were used in order to create 3D environment for the skin grafts. However, these
gelatin hybrids were not sufficient to promote the healing process especially for infected
wounds due to lack of therapeutic properties and their mechanical properties are yet to be
defined. In this research, Siam Weed therapeutic properties promote healing process and
its mechanical properties were enhanced by gelatin and electrospinning technique to
produce Siam weed-gelatin electrospun scaffold as a successful 3D skin tissue engineering
grafts. The effects of content of Siam weed extracts on the electrospun scaffolds are
determined using scanning electron microscopy, Fourier Transfom Infrared spectroscopy
and degradation study of solution properties. The results indicated that concentration
affects the solution properties such as viscosity, conductivity and ultimately the fiber
diameter. The fibrous meshes were then crosslinked with glutaraldehyde vapor and its
mechanical properties were determined by tensile . The value of tensile strength of the
crosslinked fibrous scaffold was higher than that of human skin proving that it has high
potential to be a successful skin graft when immobilized at the wound site. Cell
proliferation study of the Siam weed extracts indicated that the extracts had significantly
stimulate cell proliferation with a strong response at the concentration of 25 µg/ml
therefore giving the optimum proliferation of the cells. The results implied the possibility
of using Siam weed-gelatin fibrous scaffold as a promising candidate for tissue engineered
skin grafts |
|---|