Analyses of microscopic ossicles and mitochondrial genome of holothuria (mertensiothuria) leucospilota from Sedili Kechil, Johor, Malaysia

The white threads fish Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) or locally known as bat puntil is a widely distributed sea cucumber species in Malaysia. The species is categorised as low commercial value species, but it will be sought after if high-value species at fishing zones are...

Full description

Saved in:

Bibliographic Details
Main Author:	Badrulhisham, Nur Sabrina
Format:	Thesis
Language:	English English English
Published:	2023
Subjects:	QR Microbiology
Online Access:	http://eprints.uthm.edu.my/10989/1/24p%20NUR%20SABRINA%20BADRULHISHAM.pdf http://eprints.uthm.edu.my/10989/2/NUR%20SABRINA%20BADRULHISHAM%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/10989/3/NUR%20SABRINA%20BADRULHISHAM%20WATERMARK.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uthm-ep.10989
record_format	uketd_dc
spelling	my-uthm-ep.109892024-05-20T01:34:31Z Analyses of microscopic ossicles and mitochondrial genome of holothuria (mertensiothuria) leucospilota from Sedili Kechil, Johor, Malaysia 2023-08 Badrulhisham, Nur Sabrina QR Microbiology The white threads fish Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) or locally known as bat puntil is a widely distributed sea cucumber species in Malaysia. The species is categorised as low commercial value species, but it will be sought after if high-value species at fishing zones are depleted. Despite issues of sea cucumber taxonomic identification, the species has no representative of genetic documentation of mitogenome from Malaysia. In this study, the mitogenome of H. leucospilota collected from Malaysia, specifically from Sedili Kechil, Kota Tinggi, Johor was sequenced and annotated using Illumina Novaseq6000 next-generation sequencing (NGS) technology. A sufficient quantity and acceptable quality of DNA extract from H. leucospilota was necessary to ease the process of NGS. The calcareous ossicle shape and external morphological characteristics of H. leucospilota from Sedili Kechil were also recorded to assist early identification and provide an update of morphological characters of H. leucospilota from Sedili Kechil. The current findings showed that there were five ossicle shapes: button-shaped, rod-shaped, perforated plate, table, and anchor-shaped ossicles found in different body parts of H. leucospilota. Then, a sufficient amount of DNA extract was obtained due to an additional step and modification of centrifugation speed process during DNA filtration of column based FavorPrep DNA extraction kit. The PCGs phylogenetic analyses show that H. leucospilota is in order Holothuriida in a monophyletic group, forming sister group with H. hilla. This study recorded the first mitogenome of H. leucospilota from Malaysia. Along with the species-specific ossicle shape documentation, these findings will provide useful taxonomic and genetic reference of H. leucospilota in Malaysia 2023-08 Thesis http://eprints.uthm.edu.my/10989/ http://eprints.uthm.edu.my/10989/1/24p%20NUR%20SABRINA%20BADRULHISHAM.pdf text en public http://eprints.uthm.edu.my/10989/2/NUR%20SABRINA%20BADRULHISHAM%20COPYRIGHT%20DECLARATION.pdf text en staffonly http://eprints.uthm.edu.my/10989/3/NUR%20SABRINA%20BADRULHISHAM%20WATERMARK.pdf text en validuser mphil masters Universiti Tun Hussein Onn Malaysia Fakulti Sains Gunaan dan Teknologi
institution	Universiti Tun Hussein Onn Malaysia
collection	UTHM Institutional Repository
language	English English English
topic	QR Microbiology
spellingShingle	QR Microbiology Badrulhisham, Nur Sabrina Analyses of microscopic ossicles and mitochondrial genome of holothuria (mertensiothuria) leucospilota from Sedili Kechil, Johor, Malaysia
description	The white threads fish Holothuria (Mertensiothuria) leucospilota (Brandt, 1835) or locally known as bat puntil is a widely distributed sea cucumber species in Malaysia. The species is categorised as low commercial value species, but it will be sought after if high-value species at fishing zones are depleted. Despite issues of sea cucumber taxonomic identification, the species has no representative of genetic documentation of mitogenome from Malaysia. In this study, the mitogenome of H. leucospilota collected from Malaysia, specifically from Sedili Kechil, Kota Tinggi, Johor was sequenced and annotated using Illumina Novaseq6000 next-generation sequencing (NGS) technology. A sufficient quantity and acceptable quality of DNA extract from H. leucospilota was necessary to ease the process of NGS. The calcareous ossicle shape and external morphological characteristics of H. leucospilota from Sedili Kechil were also recorded to assist early identification and provide an update of morphological characters of H. leucospilota from Sedili Kechil. The current findings showed that there were five ossicle shapes: button-shaped, rod-shaped, perforated plate, table, and anchor-shaped ossicles found in different body parts of H. leucospilota. Then, a sufficient amount of DNA extract was obtained due to an additional step and modification of centrifugation speed process during DNA filtration of column based FavorPrep DNA extraction kit. The PCGs phylogenetic analyses show that H. leucospilota is in order Holothuriida in a monophyletic group, forming sister group with H. hilla. This study recorded the first mitogenome of H. leucospilota from Malaysia. Along with the species-specific ossicle shape documentation, these findings will provide useful taxonomic and genetic reference of H. leucospilota in Malaysia
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Badrulhisham, Nur Sabrina
author_facet	Badrulhisham, Nur Sabrina
author_sort	Badrulhisham, Nur Sabrina
title	Analyses of microscopic ossicles and mitochondrial genome of holothuria (mertensiothuria) leucospilota from Sedili Kechil, Johor, Malaysia
title_short	Analyses of microscopic ossicles and mitochondrial genome of holothuria (mertensiothuria) leucospilota from Sedili Kechil, Johor, Malaysia
title_full	Analyses of microscopic ossicles and mitochondrial genome of holothuria (mertensiothuria) leucospilota from Sedili Kechil, Johor, Malaysia
title_fullStr	Analyses of microscopic ossicles and mitochondrial genome of holothuria (mertensiothuria) leucospilota from Sedili Kechil, Johor, Malaysia
title_full_unstemmed	Analyses of microscopic ossicles and mitochondrial genome of holothuria (mertensiothuria) leucospilota from Sedili Kechil, Johor, Malaysia
title_sort	analyses of microscopic ossicles and mitochondrial genome of holothuria (mertensiothuria) leucospilota from sedili kechil, johor, malaysia
granting_institution	Universiti Tun Hussein Onn Malaysia
granting_department	Fakulti Sains Gunaan dan Teknologi
publishDate	2023
url	http://eprints.uthm.edu.my/10989/1/24p%20NUR%20SABRINA%20BADRULHISHAM.pdf http://eprints.uthm.edu.my/10989/2/NUR%20SABRINA%20BADRULHISHAM%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/10989/3/NUR%20SABRINA%20BADRULHISHAM%20WATERMARK.pdf
_version_	1804890131653459968

Analyses of microscopic ossicles and mitochondrial genome of holothuria (mertensiothuria) leucospilota from Sedili Kechil, Johor, Malaysia

Similar Items