Optimization of extraction parameters of selected Malaysian plants towards antiurolithiatic activities (IN-VITRO)

Urolithiasis has become a worldwide problem and its management depends on surgical procedures that are costly and cause reoccurrence. Anacardium occidentale (gajus), Ananas comosus (nanas), Aquilaria malaccencis (karas), Centella asiatiaca (pegaga), Ceiba petandra (kekabu), Euphorbia hirta (ara tana...

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Main Author:	Abdul Rahim, Nur Fazira
Format:	Thesis
Language:	English English English
Published:	2021
Subjects:	QK1-474.5 General Including geographical distribution
Online Access:	http://eprints.uthm.edu.my/1111/1/24p%20NUR%20FAZIRA%20BINTI%20ABDUL%20RAHIM.pdf http://eprints.uthm.edu.my/1111/2/NUR%20FAZIRA%20BINTI%20ABDUL%20RAHIM%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/1111/3/NUR%20FAZIRA%20BINTI%20ABDUL%20RAHIM%20WATERMARK.pdf
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id	my-uthm-ep.1111
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spelling	my-uthm-ep.11112021-08-22T08:39:51Z Optimization of extraction parameters of selected Malaysian plants towards antiurolithiatic activities (IN-VITRO) 2021-01 Abdul Rahim, Nur Fazira QK1-474.5 General. Including geographical distribution Urolithiasis has become a worldwide problem and its management depends on surgical procedures that are costly and cause reoccurrence. Anacardium occidentale (gajus), Ananas comosus (nanas), Aquilaria malaccencis (karas), Centella asiatiaca (pegaga), Ceiba petandra (kekabu), Euphorbia hirta (ara tanah), Ficus carica (ara), Melastoma malabthricum (senduduk), Piper sarmentosum (kaduk) and Tradescantia zebrina (kura-kura air) have been used traditionally to treat urolithiasis. However, no scientific data has been recorded on the extraction parameters. Thus, the objective of this study is to assess for the best ethnobotanical plant extract with good antiurolithiatic properties, followed by optimization of extraction parameters of selected plant extracts. The antiurolithiatic activities conducted were turbidity and titrimetric assays (in-vitro). Ethnobotanical plant extracts assessment revealed A. occidentale and A. malaccencis had the best antiurolithiatic activities as both plants had no significant difference with standard drugs (p>0.05). The extraction parameters studied were solvent concentration, extraction temperature and time that was screened using two-level factorial design and optimized by response surface methodology and central composite design. The results showed that optimum extraction condition for A. occidentale extract (0.4% ethanol, 31.5°C, 30 minutes) on both antiurolithiatic activities were exhibited 85.57±0.43% (turbidity) and 96.48±0.70% (titrimetric). Meanwhile, optimum condition of A. malaccencis extract (100% ethanol, 30.0°C, 30 minutes) demonstrated 83.58±0.75% (turbidity) and 86.57±0.80% (titrimetric). Phenols, alkaloids, saponins, flavonoids, tannins and terpernoids were identified in both optimized extracts and all have positive correlation on both antiurolithiatic assays except flavonoids. Toxicity testing using brine shrimp lethality assay presented non-toxic effect on optimized A. occidentale (LC50, 1412.50μg/mL) but A. malaccencis (LC50, 30.50μg/mL) revealed toxic effects. This study has given basic scientific evidence that optimum extraction condition is necessary to obtain optimum antiurolithiatic activity. 2021-01 Thesis http://eprints.uthm.edu.my/1111/ http://eprints.uthm.edu.my/1111/1/24p%20NUR%20FAZIRA%20BINTI%20ABDUL%20RAHIM.pdf text en public http://eprints.uthm.edu.my/1111/2/NUR%20FAZIRA%20BINTI%20ABDUL%20RAHIM%20COPYRIGHT%20DECLARATION.pdf text en staffonly http://eprints.uthm.edu.my/1111/3/NUR%20FAZIRA%20BINTI%20ABDUL%20RAHIM%20WATERMARK.pdf text en validuser mphil masters Universiti Tun Hussein Onn Malaysia Faculty of Applied Science and Technology
institution	Universiti Tun Hussein Onn Malaysia
collection	UTHM Institutional Repository
language	English English English
topic	QK1-474.5 General Including geographical distribution
spellingShingle	QK1-474.5 General Including geographical distribution Abdul Rahim, Nur Fazira Optimization of extraction parameters of selected Malaysian plants towards antiurolithiatic activities (IN-VITRO)
description	Urolithiasis has become a worldwide problem and its management depends on surgical procedures that are costly and cause reoccurrence. Anacardium occidentale (gajus), Ananas comosus (nanas), Aquilaria malaccencis (karas), Centella asiatiaca (pegaga), Ceiba petandra (kekabu), Euphorbia hirta (ara tanah), Ficus carica (ara), Melastoma malabthricum (senduduk), Piper sarmentosum (kaduk) and Tradescantia zebrina (kura-kura air) have been used traditionally to treat urolithiasis. However, no scientific data has been recorded on the extraction parameters. Thus, the objective of this study is to assess for the best ethnobotanical plant extract with good antiurolithiatic properties, followed by optimization of extraction parameters of selected plant extracts. The antiurolithiatic activities conducted were turbidity and titrimetric assays (in-vitro). Ethnobotanical plant extracts assessment revealed A. occidentale and A. malaccencis had the best antiurolithiatic activities as both plants had no significant difference with standard drugs (p>0.05). The extraction parameters studied were solvent concentration, extraction temperature and time that was screened using two-level factorial design and optimized by response surface methodology and central composite design. The results showed that optimum extraction condition for A. occidentale extract (0.4% ethanol, 31.5°C, 30 minutes) on both antiurolithiatic activities were exhibited 85.57±0.43% (turbidity) and 96.48±0.70% (titrimetric). Meanwhile, optimum condition of A. malaccencis extract (100% ethanol, 30.0°C, 30 minutes) demonstrated 83.58±0.75% (turbidity) and 86.57±0.80% (titrimetric). Phenols, alkaloids, saponins, flavonoids, tannins and terpernoids were identified in both optimized extracts and all have positive correlation on both antiurolithiatic assays except flavonoids. Toxicity testing using brine shrimp lethality assay presented non-toxic effect on optimized A. occidentale (LC50, 1412.50μg/mL) but A. malaccencis (LC50, 30.50μg/mL) revealed toxic effects. This study has given basic scientific evidence that optimum extraction condition is necessary to obtain optimum antiurolithiatic activity.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Abdul Rahim, Nur Fazira
author_facet	Abdul Rahim, Nur Fazira
author_sort	Abdul Rahim, Nur Fazira
title	Optimization of extraction parameters of selected Malaysian plants towards antiurolithiatic activities (IN-VITRO)
title_short	Optimization of extraction parameters of selected Malaysian plants towards antiurolithiatic activities (IN-VITRO)
title_full	Optimization of extraction parameters of selected Malaysian plants towards antiurolithiatic activities (IN-VITRO)
title_fullStr	Optimization of extraction parameters of selected Malaysian plants towards antiurolithiatic activities (IN-VITRO)
title_full_unstemmed	Optimization of extraction parameters of selected Malaysian plants towards antiurolithiatic activities (IN-VITRO)
title_sort	optimization of extraction parameters of selected malaysian plants towards antiurolithiatic activities (in-vitro)
granting_institution	Universiti Tun Hussein Onn Malaysia
granting_department	Faculty of Applied Science and Technology
publishDate	2021
url	http://eprints.uthm.edu.my/1111/1/24p%20NUR%20FAZIRA%20BINTI%20ABDUL%20RAHIM.pdf http://eprints.uthm.edu.my/1111/2/NUR%20FAZIRA%20BINTI%20ABDUL%20RAHIM%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/1111/3/NUR%20FAZIRA%20BINTI%20ABDUL%20RAHIM%20WATERMARK.pdf
_version_	1747830739046498304

Optimization of extraction parameters of selected Malaysian plants towards antiurolithiatic activities (IN-VITRO)

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