Characterization of botryococcus sp. and identification of carbohydrates metabolic related enzymes toward lipid production

The advancement of microalgal biofuels is faced with challenges among which is low strain performance in lipid production. Alternatively, the carbohydrate production in microalgal cells could be manipulated to increase the lipid content thereby maximizing the overall biofuel production. This work is...

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Main Author:	Abba, Zubainatu
Format:	Thesis
Language:	English English English
Published:	2019
Subjects:	QP501-801 Animal biochemistry
Online Access:	http://eprints.uthm.edu.my/139/1/24p%20ZUBAINATU%20ABBA.pdf http://eprints.uthm.edu.my/139/2/ZUBAINATU%20ABBA%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/139/3/ZUBAINATU%20ABBA%20WATERMARK.pdf
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id	my-uthm-ep.139
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spelling	my-uthm-ep.1392021-07-06T03:39:57Z Characterization of botryococcus sp. and identification of carbohydrates metabolic related enzymes toward lipid production 2019-10 Abba, Zubainatu QP501-801 Animal biochemistry The advancement of microalgal biofuels is faced with challenges among which is low strain performance in lipid production. Alternatively, the carbohydrate production in microalgal cells could be manipulated to increase the lipid content thereby maximizing the overall biofuel production. This work is aimed at determining metabolic pathway and enzymes involved in carbohydrate metabolism of Botryococcus sp. The B. sp. from Taman Negara Johor Endau Rompin was isolated and identified. Some relevant intracellular metabolites were extracted and quantified through HPLC and GC-MS analysis while extracellular metabolites excreted into the Bold basal medium were also analysed and identified through GC-MS. Enzymes involved in carbohydrate metabolism leading to lipid production in B. sp. under natural conditions were also identified through one-dimensional gel electrophoresis followed by proteomic mass spectrometry (LC-MS) and database searching. Finally, the carbohydrate to lipid metabolic pathway of B. sp. cultivated under natural conditions was determined. The total carbohydrate content was found to be 23 % per milligram biomass dry weight with monomeric sugars galactose, glucose, mannose, and arabinose. Total protein estimated for the microalgae B. sp. is 16.22 % and the lipid content was found to be 60.69 %. The extracellular metabolites constitute majorly cyclohydrocarbons, nitrogenated hydrocarbons, siloxanes, phenols and phenol derivatives. A glycolytic enzyme ‘Enolase ’which can generate phosphoenolpyruvate (PEP) and then convert it into pyruvate was identified in this study. Enolase which helps in high lipid metabolism was found in the cytoplasm and was used to construct the alternative pathway. Enolase was found to export fixed carbon (3PGA) to the cytoplasm, hence providing a shorter route to lipid production than the normal process via the plastid leading to the production of more lipid in Botryococcus. 2019-10 Thesis http://eprints.uthm.edu.my/139/ http://eprints.uthm.edu.my/139/1/24p%20ZUBAINATU%20ABBA.pdf text en public http://eprints.uthm.edu.my/139/2/ZUBAINATU%20ABBA%20COPYRIGHT%20DECLARATION.pdf text en staffonly http://eprints.uthm.edu.my/139/3/ZUBAINATU%20ABBA%20WATERMARK.pdf text en validuser phd doctoral Universiti Tun Hussein Onn Malaysia Fakulti Sains Gunaan dan Teknolgi
institution	Universiti Tun Hussein Onn Malaysia
collection	UTHM Institutional Repository
language	English English English
topic	QP501-801 Animal biochemistry
spellingShingle	QP501-801 Animal biochemistry Abba, Zubainatu Characterization of botryococcus sp. and identification of carbohydrates metabolic related enzymes toward lipid production
description	The advancement of microalgal biofuels is faced with challenges among which is low strain performance in lipid production. Alternatively, the carbohydrate production in microalgal cells could be manipulated to increase the lipid content thereby maximizing the overall biofuel production. This work is aimed at determining metabolic pathway and enzymes involved in carbohydrate metabolism of Botryococcus sp. The B. sp. from Taman Negara Johor Endau Rompin was isolated and identified. Some relevant intracellular metabolites were extracted and quantified through HPLC and GC-MS analysis while extracellular metabolites excreted into the Bold basal medium were also analysed and identified through GC-MS. Enzymes involved in carbohydrate metabolism leading to lipid production in B. sp. under natural conditions were also identified through one-dimensional gel electrophoresis followed by proteomic mass spectrometry (LC-MS) and database searching. Finally, the carbohydrate to lipid metabolic pathway of B. sp. cultivated under natural conditions was determined. The total carbohydrate content was found to be 23 % per milligram biomass dry weight with monomeric sugars galactose, glucose, mannose, and arabinose. Total protein estimated for the microalgae B. sp. is 16.22 % and the lipid content was found to be 60.69 %. The extracellular metabolites constitute majorly cyclohydrocarbons, nitrogenated hydrocarbons, siloxanes, phenols and phenol derivatives. A glycolytic enzyme ‘Enolase ’which can generate phosphoenolpyruvate (PEP) and then convert it into pyruvate was identified in this study. Enolase which helps in high lipid metabolism was found in the cytoplasm and was used to construct the alternative pathway. Enolase was found to export fixed carbon (3PGA) to the cytoplasm, hence providing a shorter route to lipid production than the normal process via the plastid leading to the production of more lipid in Botryococcus.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Abba, Zubainatu
author_facet	Abba, Zubainatu
author_sort	Abba, Zubainatu
title	Characterization of botryococcus sp. and identification of carbohydrates metabolic related enzymes toward lipid production
title_short	Characterization of botryococcus sp. and identification of carbohydrates metabolic related enzymes toward lipid production
title_full	Characterization of botryococcus sp. and identification of carbohydrates metabolic related enzymes toward lipid production
title_fullStr	Characterization of botryococcus sp. and identification of carbohydrates metabolic related enzymes toward lipid production
title_full_unstemmed	Characterization of botryococcus sp. and identification of carbohydrates metabolic related enzymes toward lipid production
title_sort	characterization of botryococcus sp. and identification of carbohydrates metabolic related enzymes toward lipid production
granting_institution	Universiti Tun Hussein Onn Malaysia
granting_department	Fakulti Sains Gunaan dan Teknolgi
publishDate	2019
url	http://eprints.uthm.edu.my/139/1/24p%20ZUBAINATU%20ABBA.pdf http://eprints.uthm.edu.my/139/2/ZUBAINATU%20ABBA%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/139/3/ZUBAINATU%20ABBA%20WATERMARK.pdf
_version_	1747830538612244480

Characterization of botryococcus sp. and identification of carbohydrates metabolic related enzymes toward lipid production

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