Bioremediation of heavy metal polluted water using immobilized freshwater green microalga, botryococcus sp.

Heavy metal containing wastewater are regarded as highly toxic to the aquatic environment and to life in general due to their bio-accumulating, cytotoxic, mutagenic and carcinogenic effects on life. Bioremediation is the use of biological materials (e.g. microalgae) in the removal of toxic com...

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Main Author:	Joan Iye, Onalo
Format:	Thesis
Language:	English English English
Published:	2015
Subjects:	TD172-193.5 Environmental pollution
Online Access:	http://eprints.uthm.edu.my/1439/1/24p%20ONALO%20JOAN%20IYE.pdf http://eprints.uthm.edu.my/1439/2/ONALO%20JOAN%20IYE%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/1439/3/ONALO%20JOAN%20IYE%20WATERMARK.pdf
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spelling	my-uthm-ep.14392021-10-03T07:22:04Z Bioremediation of heavy metal polluted water using immobilized freshwater green microalga, botryococcus sp. 2015-05 Joan Iye, Onalo TD172-193.5 Environmental pollution Heavy metal containing wastewater are regarded as highly toxic to the aquatic environment and to life in general due to their bio-accumulating, cytotoxic, mutagenic and carcinogenic effects on life. Bioremediation is the use of biological materials (e.g. microalgae) in the removal of toxic compounds from the environment such as the heavy metals which is considered more cost effective and environmentally friendly when compared to the physical and chemical methods. The present study was undertaken to check for the heavy metal bioremoval efficiency of free and immobilized Botryococcus sp. Four heavy metals were studied and the free cells efficiently reduced Chromium which is equivalent to 94%, followed by Copper (45%), Arsenic (9%) and Cadmium (2%). For the immobilized biomass, the highest (P<0.05) removal efficiency was recorded in the highest biomass concentration (i.e. 15 beads/ml) for Cadmium, Arsenic and Chromium at 76%, 68% and 67%. Whereas, the highest (P<0.05) removal of copper was observed in the blank alginate beads at 84%. The positive control (free cells) recorded the highest (P<0.05) reduction for biological oxygen demand (BOD) whereas, the 15 beads/ml gave the highest (P<0.05) reduction for control gave the highest (P<0.05) reduction for the Chemical oxygen demand (COD). In the LD50 experiment, immobilized biomass harvested from the bioremoval study experiment were used on fishes for toxicity testing. A total of 100% mortality was recorded in the positive control after 24 hours whereas, 3% mortality was observed in negative control and in the 10 beads/ml treatment after 72 hours. No mortality was found in any other treatment after a period of 96 hours. The results obtained from this study suggests that, immobilized cells of Botryococcus sp. is efficient in the bioremoval of heavy metals from contaminated waters and also have great potential in the biotransformation of toxic compounds to less-toxic forms. 2015-05 Thesis http://eprints.uthm.edu.my/1439/ http://eprints.uthm.edu.my/1439/1/24p%20ONALO%20JOAN%20IYE.pdf text en public http://eprints.uthm.edu.my/1439/2/ONALO%20JOAN%20IYE%20COPYRIGHT%20DECLARATION.pdf text en staffonly http://eprints.uthm.edu.my/1439/3/ONALO%20JOAN%20IYE%20WATERMARK.pdf text en validuser mphil masters Universiti Tun Hussein Onn Malaysia Fakulti Sains Gunaan dan Teknologi
institution	Universiti Tun Hussein Onn Malaysia
collection	UTHM Institutional Repository
language	English English English
topic	TD172-193.5 Environmental pollution
spellingShingle	TD172-193.5 Environmental pollution Joan Iye, Onalo Bioremediation of heavy metal polluted water using immobilized freshwater green microalga, botryococcus sp.
description	Heavy metal containing wastewater are regarded as highly toxic to the aquatic environment and to life in general due to their bio-accumulating, cytotoxic, mutagenic and carcinogenic effects on life. Bioremediation is the use of biological materials (e.g. microalgae) in the removal of toxic compounds from the environment such as the heavy metals which is considered more cost effective and environmentally friendly when compared to the physical and chemical methods. The present study was undertaken to check for the heavy metal bioremoval efficiency of free and immobilized Botryococcus sp. Four heavy metals were studied and the free cells efficiently reduced Chromium which is equivalent to 94%, followed by Copper (45%), Arsenic (9%) and Cadmium (2%). For the immobilized biomass, the highest (P<0.05) removal efficiency was recorded in the highest biomass concentration (i.e. 15 beads/ml) for Cadmium, Arsenic and Chromium at 76%, 68% and 67%. Whereas, the highest (P<0.05) removal of copper was observed in the blank alginate beads at 84%. The positive control (free cells) recorded the highest (P<0.05) reduction for biological oxygen demand (BOD) whereas, the 15 beads/ml gave the highest (P<0.05) reduction for control gave the highest (P<0.05) reduction for the Chemical oxygen demand (COD). In the LD50 experiment, immobilized biomass harvested from the bioremoval study experiment were used on fishes for toxicity testing. A total of 100% mortality was recorded in the positive control after 24 hours whereas, 3% mortality was observed in negative control and in the 10 beads/ml treatment after 72 hours. No mortality was found in any other treatment after a period of 96 hours. The results obtained from this study suggests that, immobilized cells of Botryococcus sp. is efficient in the bioremoval of heavy metals from contaminated waters and also have great potential in the biotransformation of toxic compounds to less-toxic forms.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Joan Iye, Onalo
author_facet	Joan Iye, Onalo
author_sort	Joan Iye, Onalo
title	Bioremediation of heavy metal polluted water using immobilized freshwater green microalga, botryococcus sp.
title_short	Bioremediation of heavy metal polluted water using immobilized freshwater green microalga, botryococcus sp.
title_full	Bioremediation of heavy metal polluted water using immobilized freshwater green microalga, botryococcus sp.
title_fullStr	Bioremediation of heavy metal polluted water using immobilized freshwater green microalga, botryococcus sp.
title_full_unstemmed	Bioremediation of heavy metal polluted water using immobilized freshwater green microalga, botryococcus sp.
title_sort	bioremediation of heavy metal polluted water using immobilized freshwater green microalga, botryococcus sp.
granting_institution	Universiti Tun Hussein Onn Malaysia
granting_department	Fakulti Sains Gunaan dan Teknologi
publishDate	2015
url	http://eprints.uthm.edu.my/1439/1/24p%20ONALO%20JOAN%20IYE.pdf http://eprints.uthm.edu.my/1439/2/ONALO%20JOAN%20IYE%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/1439/3/ONALO%20JOAN%20IYE%20WATERMARK.pdf
_version_	1747830790058672128

Bioremediation of heavy metal polluted water using immobilized freshwater green microalga, botryococcus sp.

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