Evaluation of anti-tuberculosis potentials of selected medicinal plants in Endau Rompin, Johor, Malaysia

Tuberculosis (TB) remains an escalating health crisis globally which prompts new approaches to find more effective therapeutic strategies. Medicinal plants of Malaysia have a significant role to play in being able to provide new therapeutic remedies. The local people of Kampung Peta (Jakun tribe), E...

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Main Author:	Sanusi, Shuaibu Babaji
Format:	Thesis
Language:	English English English
Published:	2018
Subjects:	QR Microbiology
Online Access:	http://eprints.uthm.edu.my/261/1/24p%20SHUAIBU%20BABAJI%20SANUSI.pdf http://eprints.uthm.edu.my/261/2/SHUAIBU%20BABAJI%20SANUSI%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/261/3/SHUAIBU%20BABAJI%20SANUSI%20WATERMARK.pdf
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spelling	my-uthm-ep.2612021-07-15T01:11:40Z Evaluation of anti-tuberculosis potentials of selected medicinal plants in Endau Rompin, Johor, Malaysia 2018-06 Sanusi, Shuaibu Babaji QR Microbiology Tuberculosis (TB) remains an escalating health crisis globally which prompts new approaches to find more effective therapeutic strategies. Medicinal plants of Malaysia have a significant role to play in being able to provide new therapeutic remedies. The local people of Kampung Peta (Jakun tribe), Endau Rompin claimed that local preparations of some plants are used to treat symptoms of tuberculosis. There is a need to validate the claim by tradition healers scientifically. The aim of this research is to search for anti-TB from plants of Taman Negara Johor Endau-Rompin, exploiting the traditional medical practices of the Jakun people. Aqueous and organic extracts of these plant species were screened for their antimycobacterial activity using agar disk diffusion assay, Tetrazolium Microplate Assay and agar plate assay against Mycobacterium smegmatis. The effect of the extract on mycobacterial cell at the cellular level was investigated upon treatment with the crude extracts via time-kill analysis, leakage of compound absorbing at 280nm, and field emission-scanning electron microscopy (FE-SEM). The findings revealed that methanol extract of Nepenthes ampularia displayed the largest zone of inhibition (DIZ=18.67 ± 0.58 mm). Ethyl acetate extract of Musa gracilis and hexane extract of N. ampularia exhibited the lowest minimum inhibitory concentration (MIC=0.39 mg/mL). Hexane extract of N. ampularia showed the lowest minimum bactericidal concentration (MBC= 1.56 mg/mL). At 3-fold of MIC, hexane extract of N. ampularia, ethyl acetate extract of M. gracilis and ethyl acetate extract of N. ampularia killed the entire bacterial cell within 8 h of exposure by causing the cell lysis. The GC-MS analysis revealed the presence of phytoconstituents that might contribute to the antimycobacterial effect. The study scientifically justified the use of the selected medicinal plant species by Jakun people. Further studies on N. ampularia and M. gracilis could lead to the development of new anti-TB drugs. 2018-06 Thesis http://eprints.uthm.edu.my/261/ http://eprints.uthm.edu.my/261/1/24p%20SHUAIBU%20BABAJI%20SANUSI.pdf text en public http://eprints.uthm.edu.my/261/2/SHUAIBU%20BABAJI%20SANUSI%20COPYRIGHT%20DECLARATION.pdf text en staffonly http://eprints.uthm.edu.my/261/3/SHUAIBU%20BABAJI%20SANUSI%20WATERMARK.pdf text en validuser phd doctoral Universiti Tun Hussein Onn Malaysia Fakulti Sains Gunaan dan Teknolgi
institution	Universiti Tun Hussein Onn Malaysia
collection	UTHM Institutional Repository
language	English English English
topic	QR Microbiology
spellingShingle	QR Microbiology Sanusi, Shuaibu Babaji Evaluation of anti-tuberculosis potentials of selected medicinal plants in Endau Rompin, Johor, Malaysia
description	Tuberculosis (TB) remains an escalating health crisis globally which prompts new approaches to find more effective therapeutic strategies. Medicinal plants of Malaysia have a significant role to play in being able to provide new therapeutic remedies. The local people of Kampung Peta (Jakun tribe), Endau Rompin claimed that local preparations of some plants are used to treat symptoms of tuberculosis. There is a need to validate the claim by tradition healers scientifically. The aim of this research is to search for anti-TB from plants of Taman Negara Johor Endau-Rompin, exploiting the traditional medical practices of the Jakun people. Aqueous and organic extracts of these plant species were screened for their antimycobacterial activity using agar disk diffusion assay, Tetrazolium Microplate Assay and agar plate assay against Mycobacterium smegmatis. The effect of the extract on mycobacterial cell at the cellular level was investigated upon treatment with the crude extracts via time-kill analysis, leakage of compound absorbing at 280nm, and field emission-scanning electron microscopy (FE-SEM). The findings revealed that methanol extract of Nepenthes ampularia displayed the largest zone of inhibition (DIZ=18.67 ± 0.58 mm). Ethyl acetate extract of Musa gracilis and hexane extract of N. ampularia exhibited the lowest minimum inhibitory concentration (MIC=0.39 mg/mL). Hexane extract of N. ampularia showed the lowest minimum bactericidal concentration (MBC= 1.56 mg/mL). At 3-fold of MIC, hexane extract of N. ampularia, ethyl acetate extract of M. gracilis and ethyl acetate extract of N. ampularia killed the entire bacterial cell within 8 h of exposure by causing the cell lysis. The GC-MS analysis revealed the presence of phytoconstituents that might contribute to the antimycobacterial effect. The study scientifically justified the use of the selected medicinal plant species by Jakun people. Further studies on N. ampularia and M. gracilis could lead to the development of new anti-TB drugs.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Sanusi, Shuaibu Babaji
author_facet	Sanusi, Shuaibu Babaji
author_sort	Sanusi, Shuaibu Babaji
title	Evaluation of anti-tuberculosis potentials of selected medicinal plants in Endau Rompin, Johor, Malaysia
title_short	Evaluation of anti-tuberculosis potentials of selected medicinal plants in Endau Rompin, Johor, Malaysia
title_full	Evaluation of anti-tuberculosis potentials of selected medicinal plants in Endau Rompin, Johor, Malaysia
title_fullStr	Evaluation of anti-tuberculosis potentials of selected medicinal plants in Endau Rompin, Johor, Malaysia
title_full_unstemmed	Evaluation of anti-tuberculosis potentials of selected medicinal plants in Endau Rompin, Johor, Malaysia
title_sort	evaluation of anti-tuberculosis potentials of selected medicinal plants in endau rompin, johor, malaysia
granting_institution	Universiti Tun Hussein Onn Malaysia
granting_department	Fakulti Sains Gunaan dan Teknolgi
publishDate	2018
url	http://eprints.uthm.edu.my/261/1/24p%20SHUAIBU%20BABAJI%20SANUSI.pdf http://eprints.uthm.edu.my/261/2/SHUAIBU%20BABAJI%20SANUSI%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/261/3/SHUAIBU%20BABAJI%20SANUSI%20WATERMARK.pdf
_version_	1747830567332741120

Evaluation of anti-tuberculosis potentials of selected medicinal plants in Endau Rompin, Johor, Malaysia

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