Green synthesis, characterization and in-vitro bioactivities of gold nanoparticles mediated by turmeric crude extract and curcumin
In the present study, green synthesis and characterization of gold nanoparticles (GNPs) using the ethanolic crude extract of turmeric (Tur-CE) and curcumin (Cur) have been described. Antioxidant, anticholinesterase (anti-ChE), and antiurolithiatic activities of the extracts and the GNPs were test...
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| Format: | Thesis |
| Language: | English English English |
| Published: |
2021
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| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/3933/1/24p%20SAMEERA%20ABBAS.pdf http://eprints.uthm.edu.my/3933/2/SAMEERA%20ABBAS%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/3933/3/SAMEERA%20ABBAS%20WATERMARK.pdf |
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| Summary: | In the present study, green synthesis and characterization of gold nanoparticles
(GNPs) using the ethanolic crude extract of turmeric (Tur-CE) and curcumin (Cur)
have been described. Antioxidant, anticholinesterase (anti-ChE), and antiurolithiatic
activities of the extracts and the GNPs were tested in vitro and Pearson’s correlation
analysis was performed. The optimization of GNP synthesis was performed using
Tur-CE (2 % of 10 mg/mL) and curcumin (0.5 % of 10 mg/mL) at a reactant ratio
(Tur-CE or Cur: HAuCl4) of 1:4, pH 6 and 12 of Tur-CE and curcumin, respectively.
The other conditions include the concentration of HAuCl4 (0.25 mM) and different
reaction temperatures (25, 40, 55 and 70 °C). FESEM analysis of GNPs synthesized
at 25 °C by using Tur-CE (TP6.25) and curcumin (CP12.25) revealed the size range
of 11-40 and 31-100 nm, respectively. The highest antioxidant activity was recorded
for TP6.25, that is, 82.60, 79.60 and 12.22 % for ABTS, DPPH and FRAP,
respectively, and anti-ChE activity at 75.10 and 74.33 % for AChE and BChE,
respectively, However, the maximum % I for ABTS, DPPH, and FRAP, that is,
87.20, 86.00 % and 19.36 µg FSE, respectively, was shown by positive control
ascorbic acid, and for AChE and BChE inhibition, 86.69 and 89.30 %, respectively,
by galanthamine. TP6.25 also showed good antiurolithiatic activity as indicated by
absorbance value of 0.198 and 0.164 in nucleation and crystallization assays,
respectively. Pearson correlation revealed a positive correlation between antioxidant
and anti-ChE, as well as antioxidant and antiurolithiatic activities. The GNPs were
haemocompatible as TP6.25 and CP12.25 induced 1.172 and 0.763 % haemolysis,
respectively, at the highest concentration of 500 µL in haemolysis assay compared to
99.85 % haemolysis by positive control. In conclusion, Tur-GNPs and Cur-GNPs
possess good antioxidant, anticholinesterase and antiurolithiatic properties, in
addition to being non-haemolytic, may be an option to further explore their
therapeutic potential as antioxidant, anticholinesterase, and antiurolithiatic agents in
neurodegeneration and urolithiasis. |
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