Fabrication of graphene-based resistive sensor for eschericia coli bacteria sensing

The presence of fecal coliform bacteria like Escherichia coli (E. coli) in aquatic environments indicates that the water has been contaminated with the fecal material of human or animals. The risk of health threatening to humans become high when E. coli bacteria are exposed to water, not only causes...

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Main Author:	Wibowo, Kusnanto Mukti
Format:	Thesis
Language:	English English English
Published:	2018
Subjects:	T173.2-174.5 Technological change
Online Access:	http://eprints.uthm.edu.my/417/1/24p%20KUSNANTO%20MUKTI%20WIBOWO.pdf http://eprints.uthm.edu.my/417/2/KUSNANTO%20MUKTI%20WIBOWO%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/417/3/KUSNANTO%20MUKTI%20WIBOWO%20WATERMARK.pdf
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spelling	my-uthm-ep.4172021-07-25T02:47:45Z Fabrication of graphene-based resistive sensor for eschericia coli bacteria sensing 2018-09 Wibowo, Kusnanto Mukti T173.2-174.5 Technological change The presence of fecal coliform bacteria like Escherichia coli (E. coli) in aquatic environments indicates that the water has been contaminated with the fecal material of human or animals. The risk of health threatening to humans become high when E. coli bacteria are exposed to water, not only causes diarrhea but also lead to death if not treated immediately. Many existing sensors to detect E. coli bacteria are not portable and expensive, while the conventional methods are time consuming and need the sterile procedure. Therefore, it is important to develop a sensor to detect E. coli rapidly, portable and user friendly. In this work, bacteria sensor based on resistivity was developed using graphene nanostructure as a sensing layer, since carbon material like graphene has been known to have a biocompatibility and excellent electrical property. The E. coli bacteria used in this experiment were collected from domestic wastewater and commercial of E. coli strain no. ATCCC 25922. A biochemical test was performed to detect the presence of E. coli in wastewater. The physical characterization of the bacteria was carried out by using FESEM and it was found that the E. coli has size ranging from 1.4-1.6 μm in length. The wettability properties of graphene confirmed that graphene surface is hydrophobic with contact angle of 108.9o which is suitable for bacterial detection. Raman spectroscopy measurements shows that the ratio of G peak and D peak intensity increase due to increase in the number of E. coli. Moreover, the electrical property of graphene shows increasing the number of the bacteria from 4 to 273 colony forming-unit (cfu) result decreasing the resistance from 4.371 to 3.903 ohm gradually. Finally, the sensor was successfully designed using SolidWorks and fabricated by integrating graphene film as a sensing layer with Arduino micro-controller. The validation of the sensor was performed by comparing the data obtained by sensor device and plate culture and has been found to have an error rate of ±12.3%. 2018-09 Thesis http://eprints.uthm.edu.my/417/ http://eprints.uthm.edu.my/417/1/24p%20KUSNANTO%20MUKTI%20WIBOWO.pdf text en public http://eprints.uthm.edu.my/417/2/KUSNANTO%20MUKTI%20WIBOWO%20COPYRIGHT%20DECLARATION.pdf text en staffonly http://eprints.uthm.edu.my/417/3/KUSNANTO%20MUKTI%20WIBOWO%20WATERMARK.pdf text en validuser mphil masters Universiti Tun Hussein Onn Malaysia Faculty of Electrical and Electronic Engineering
institution	Universiti Tun Hussein Onn Malaysia
collection	UTHM Institutional Repository
language	English English English
topic	T173.2-174.5 Technological change
spellingShingle	T173.2-174.5 Technological change Wibowo, Kusnanto Mukti Fabrication of graphene-based resistive sensor for eschericia coli bacteria sensing
description	The presence of fecal coliform bacteria like Escherichia coli (E. coli) in aquatic environments indicates that the water has been contaminated with the fecal material of human or animals. The risk of health threatening to humans become high when E. coli bacteria are exposed to water, not only causes diarrhea but also lead to death if not treated immediately. Many existing sensors to detect E. coli bacteria are not portable and expensive, while the conventional methods are time consuming and need the sterile procedure. Therefore, it is important to develop a sensor to detect E. coli rapidly, portable and user friendly. In this work, bacteria sensor based on resistivity was developed using graphene nanostructure as a sensing layer, since carbon material like graphene has been known to have a biocompatibility and excellent electrical property. The E. coli bacteria used in this experiment were collected from domestic wastewater and commercial of E. coli strain no. ATCCC 25922. A biochemical test was performed to detect the presence of E. coli in wastewater. The physical characterization of the bacteria was carried out by using FESEM and it was found that the E. coli has size ranging from 1.4-1.6 μm in length. The wettability properties of graphene confirmed that graphene surface is hydrophobic with contact angle of 108.9o which is suitable for bacterial detection. Raman spectroscopy measurements shows that the ratio of G peak and D peak intensity increase due to increase in the number of E. coli. Moreover, the electrical property of graphene shows increasing the number of the bacteria from 4 to 273 colony forming-unit (cfu) result decreasing the resistance from 4.371 to 3.903 ohm gradually. Finally, the sensor was successfully designed using SolidWorks and fabricated by integrating graphene film as a sensing layer with Arduino micro-controller. The validation of the sensor was performed by comparing the data obtained by sensor device and plate culture and has been found to have an error rate of ±12.3%.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Wibowo, Kusnanto Mukti
author_facet	Wibowo, Kusnanto Mukti
author_sort	Wibowo, Kusnanto Mukti
title	Fabrication of graphene-based resistive sensor for eschericia coli bacteria sensing
title_short	Fabrication of graphene-based resistive sensor for eschericia coli bacteria sensing
title_full	Fabrication of graphene-based resistive sensor for eschericia coli bacteria sensing
title_fullStr	Fabrication of graphene-based resistive sensor for eschericia coli bacteria sensing
title_full_unstemmed	Fabrication of graphene-based resistive sensor for eschericia coli bacteria sensing
title_sort	fabrication of graphene-based resistive sensor for eschericia coli bacteria sensing
granting_institution	Universiti Tun Hussein Onn Malaysia
granting_department	Faculty of Electrical and Electronic Engineering
publishDate	2018
url	http://eprints.uthm.edu.my/417/1/24p%20KUSNANTO%20MUKTI%20WIBOWO.pdf http://eprints.uthm.edu.my/417/2/KUSNANTO%20MUKTI%20WIBOWO%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/417/3/KUSNANTO%20MUKTI%20WIBOWO%20WATERMARK.pdf
_version_	1747830605010173952

Fabrication of graphene-based resistive sensor for eschericia coli bacteria sensing

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