Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B

Mixing and dilution are essential procedures in pharmaceutical operation to process two or more components in a separate or thoroughly mixed condition until homogenous solution was obtained. However, conventional serial dilution method used in laboratory assessment causes high usage of reagents, hig...

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Main Author:	Sundra, Sargunan
Format:	Thesis
Language:	English English English
Published:	2018
Subjects:	TP155-156 Chemical engineering
Online Access:	http://eprints.uthm.edu.my/449/1/24p%20SARGUNAN%20AL%20SUNDRA.pdf http://eprints.uthm.edu.my/449/2/SARGUNAN%20AL%20SUNDRA%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/449/3/SARGUNAN%20AL%20SUNDRA%20WATERMARK.pdf
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spelling	my-uthm-ep.4492021-07-25T06:32:24Z Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B 2018-06 Sundra, Sargunan TP155-156 Chemical engineering Mixing and dilution are essential procedures in pharmaceutical operation to process two or more components in a separate or thoroughly mixed condition until homogenous solution was obtained. However, conventional serial dilution method used in laboratory assessment causes high usage of reagents, higher complexity procedures and costly. Micromixing method provides a better platform that enables mixing and dilution of liquid-based reagents which is convenient solutions preparation, easy liquid handling and time-saving. In this study, a polydimethylsiloxane (PDMS) micromixer was designed, simulated and prototyped using vinyl tape method and successfully applied to mix and dilute Cytochalasin-B in culture media (CB-DMEM, 30.0 μM) with 0.05 % ethanol solutions (diluent) to produce four different concentrations of CB-DMEM (5.3, 10.6, 14.8, and 20.2 μM). The different concentrations of CB-DMEM were applied on to ORL-48 microtissues produced by using flicking technique. The morphological responses, cell viability and cell proliferation of ORL-48 monolayer cells (2D) and microtissues (3D) treated in four different CB concentrations were assessed via phase contrast microscopy, live/dead staining and Alamar Blue® staining respectively. The results show that both 2D and 3D of ORL-48 microtissues were only morphologically affected (fibroblastic spreading to round shape) while cell viability and cell proliferation show that CB treatment solely does not causes apoptosis (≈ 90 % cells are alive and able to proliferate). The micromixer employed in solution preparation of CB-DMEM (5.3, 10.6, 14.8, and 20.2 μM) provide a convenient and faster method to prepare cytochemical solution for drug screening and experiments. Besides that, application of micromixer consumes less volume of reagents and cost efficient. 2018-06 Thesis http://eprints.uthm.edu.my/449/ http://eprints.uthm.edu.my/449/1/24p%20SARGUNAN%20AL%20SUNDRA.pdf text en public http://eprints.uthm.edu.my/449/2/SARGUNAN%20AL%20SUNDRA%20COPYRIGHT%20DECLARATION.pdf text en staffonly http://eprints.uthm.edu.my/449/3/SARGUNAN%20AL%20SUNDRA%20WATERMARK.pdf text en validuser mphil masters Universiti Tun Hussein Onn Malaysia Faculty of Electrical and Electronic Engineering
institution	Universiti Tun Hussein Onn Malaysia
collection	UTHM Institutional Repository
language	English English English
topic	TP155-156 Chemical engineering
spellingShingle	TP155-156 Chemical engineering Sundra, Sargunan Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B
description	Mixing and dilution are essential procedures in pharmaceutical operation to process two or more components in a separate or thoroughly mixed condition until homogenous solution was obtained. However, conventional serial dilution method used in laboratory assessment causes high usage of reagents, higher complexity procedures and costly. Micromixing method provides a better platform that enables mixing and dilution of liquid-based reagents which is convenient solutions preparation, easy liquid handling and time-saving. In this study, a polydimethylsiloxane (PDMS) micromixer was designed, simulated and prototyped using vinyl tape method and successfully applied to mix and dilute Cytochalasin-B in culture media (CB-DMEM, 30.0 μM) with 0.05 % ethanol solutions (diluent) to produce four different concentrations of CB-DMEM (5.3, 10.6, 14.8, and 20.2 μM). The different concentrations of CB-DMEM were applied on to ORL-48 microtissues produced by using flicking technique. The morphological responses, cell viability and cell proliferation of ORL-48 monolayer cells (2D) and microtissues (3D) treated in four different CB concentrations were assessed via phase contrast microscopy, live/dead staining and Alamar Blue® staining respectively. The results show that both 2D and 3D of ORL-48 microtissues were only morphologically affected (fibroblastic spreading to round shape) while cell viability and cell proliferation show that CB treatment solely does not causes apoptosis (≈ 90 % cells are alive and able to proliferate). The micromixer employed in solution preparation of CB-DMEM (5.3, 10.6, 14.8, and 20.2 μM) provide a convenient and faster method to prepare cytochemical solution for drug screening and experiments. Besides that, application of micromixer consumes less volume of reagents and cost efficient.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Sundra, Sargunan
author_facet	Sundra, Sargunan
author_sort	Sundra, Sargunan
title	Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B
title_short	Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B
title_full	Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B
title_fullStr	Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B
title_full_unstemmed	Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B
title_sort	development of a micromixing system for treatment of orl 48 microtissues in different concentrations of cytochalasin b
granting_institution	Universiti Tun Hussein Onn Malaysia
granting_department	Faculty of Electrical and Electronic Engineering
publishDate	2018
url	http://eprints.uthm.edu.my/449/1/24p%20SARGUNAN%20AL%20SUNDRA.pdf http://eprints.uthm.edu.my/449/2/SARGUNAN%20AL%20SUNDRA%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/449/3/SARGUNAN%20AL%20SUNDRA%20WATERMARK.pdf
_version_	1747830612841988096

Development of a micromixing system for treatment of ORL 48 microtissues in different concentrations of cytochalasin B

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