Study of low voltage electroporation technique on hela cell treated with neolamarckia cadamba extracts daing
Electroporation is a process of creating pores on cell by exposing the cell with electric field. This process increases the permeability of the cell. Neolamarckia Cadamba is a natural source that is widely used in the medical arena due to its pharmacological properties. Neolamarckia Cadamba known as...
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| Format: | Thesis |
| Language: | English English English |
| Published: |
2019
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| Online Access: | http://eprints.uthm.edu.my/485/1/24p%20DAING%20NOOR%20FARHANAH%20MOHAMAD%20ISHAK.pdf http://eprints.uthm.edu.my/485/2/DAING%20NOOR%20FARHANAH%20MOHAMAD%20ISHAK%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/485/3/DAING%20NOOR%20FARHANAH%20MOHAMAD%20ISHAK%20WATERMARK.pdf |
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| Summary: | Electroporation is a process of creating pores on cell by exposing the cell with electric field. This process increases the permeability of the cell. Neolamarckia Cadamba is a natural source that is widely used in the medical arena due to its pharmacological properties. Neolamarckia Cadamba known as kelempayang to Malaysian people. Its mainly planted in the states of Pahang, Perak, Sabah and Sarawak. Electrochemotherapy is a combination of the electroporation process and chemotherapeutic drugs. The Standard electric field parameters for Electrochemotherapy (SEFPECT) are 1000V/cm of pulse amplitude, 100μs of pulse duration, 8 numbers of pulses and 1Hz of frequency. Electrochemotherapy process causes transient burns to patient due to the intensity of pulse amplitude used, which is 1000V/cm. The use of chemotherapeutic various drugs gives side effect to the patient. To overcome the problem, this research suggests to lower the pulse amplitude and longer pulse duration plus combining natural extract of Neolamarckia Cadamba Leaves. In this study, the combination of electroporation process and natural extract is conducted in vitro for 48 hours. The cell used is cervical cancer cell (HeLa cell) and the natural extract from Neolamarckia Cadamba Leaves. The experiment was started to determine the best parameter of pulse amplitude and pulse duration that gives lower viability percentage of HeLa cell. The pulse amplitude started with a 200V/cm, 400V/cm, 600V/cm, 800V/cm and 1000V/cm and the pulse duration at 100μs, 500μs, 1ms, 2ms and 5ms and the result revealed that the best parameter at 600V/cm of pulse amplitude and 5ms of pulse duration. Then the IC50 value of Neolamarckia Cadamba Leaves extract were tested and found to be at 40μg/ml. The findings of these studies have shown positive respond on the combination of these two methods. This was proven by the reduction in the percentage of anti proliferation of HeLa cell where with electroporation 30.08% cell can survive and by combining electroporation and extact of Neolamarckia Cadamba leaves only 2.12% HeLa cell can survive. |
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