Study of melissopalynological, physical and chemical properties, antioxidant and xanthine oxidase inhibition activity of heterotrigona itama honey in Johor, Malaysia
Stingless bee honey or known as ‘madu kelulut’ by Malaysian native started to receive attention due to their acclaimed medicinal values and health benefits. To date, there are limited reports of stingless bee honey compositions available in Malaysia. Therefore, the objective of this study was to...
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| Format: | Thesis |
| Language: | English English English |
| Published: |
2019
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| Subjects: | |
| Online Access: | http://eprints.uthm.edu.my/553/1/24p%20MAHANI%20MAJID.pdf http://eprints.uthm.edu.my/553/2/MAHANI%20MAJID%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/553/3/MAHANI%20MAJID%20WATERMARK.pdf |
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| Summary: | Stingless bee honey or known as ‘madu kelulut’ by Malaysian native started to
receive attention due to their acclaimed medicinal values and health benefits. To date,
there are limited reports of stingless bee honey compositions available in Malaysia.
Therefore, the objective of this study was to identify the plant sources, physical and
chemical properties, antioxidant and xanthine oxidase inhibition activity of
Heterotrigona itama honey from six selected area in Johor, Malaysia. Plant sources
of honey were identified by melissopalynological analysis, and multiply methods
were conducted to investigate the physical and chemical properties of honey. Three
different antioxidant assays (DPPH scavenging, ABTS decolourization and FRAP),
together with phenolic compounds, total phenolic and flavonoid content were also
evaluated. Then, enzyme activity was tested by xanthine oxidase inhibition assay.
The pollen identification of honeys has clustered them into unifloral and multifloral
honey where Cocos nucifera and Rhizophora mucronata species were identified as
predominant pollen types. All analyzed honey samples meet the national standard
requirements (electrical conductivity: 0.57 mS/cm; pH value: 3.40, colour intensity:
692 mAU, moisture content: 28.95 g/100g, ash content: 0.37 g/100g, sugar content:
20.25 g/100g) which showed them as a good quality product. HPLC screening
detected six phenolic acids (chlorogenic acid, epicatechin, P-Coumaric acid, rutin,
catechin and protocatheuic) which present in various combination. Unifloral honey
(R. mucronata) possessed the highest phenolic (413.94 mg GAE/100g) and flavonoid
(179.36 mg RE/100g) content and have the highest antioxidant (DPPH: 74.03 %;
ABTS: 65.02 %; FRAP: 1401.80 uM Fe(II)/100g) and enzyme inhibition (IC50: 30.0
mg/mL) activity. This study highlights the importance of plant sources of honey
which may increase its commercial value and could be significant contribution to the
database for the sustainability of stingless bee industry in Malaysia. |
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