DNA metabarcoding and phytochemical analysis of plants consumed by banded langur (presbytis femoralis) in Johor
Banded langur (Presbytis femoralis) is a leaf-eating monkey that is now classified as vulnerable in IUCN Red List and to date, there are less than 500 individuals of P. femoralis that can be observed in some fragmented areas in Johor. Currently, banded langur is facing a problem in which there is...
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my-uthm-ep.64352022-02-05T07:25:22Z DNA metabarcoding and phytochemical analysis of plants consumed by banded langur (presbytis femoralis) in Johor 2021-09 Haris, Norhidayah QC Physics T Technology (General) Banded langur (Presbytis femoralis) is a leaf-eating monkey that is now classified as vulnerable in IUCN Red List and to date, there are less than 500 individuals of P. femoralis that can be observed in some fragmented areas in Johor. Currently, banded langur is facing a problem in which there is no comprehensive record of their plants’ selection and there is no information on phytochemical compositions in their diets. This data is crucial for their conservation plan along with solving the human-wildlife conflict, but it is very challenging to identify the plants consumed by them through direct observations. Thus, the objectives of this study are to investigate the plant selection by P. femoralis based on a DNA metabarcoding approach using the trnL region of chloroplast DNA and to identify the phytochemical compounds in the selected plant species consumed for bioprospecting purposes. Through the sequencing method of four faecal samples collected, a total of 140611 operational taxonomic units (OTUs) were detected which were assigned to 38 families and 71 genera of plants. The plants from the family of Fabaceae (3.03%) contributed to the most plant food consumed, followed by the family of Euphorbiaceae (2.63%) and Malvaceae (1.10%). As for the phytochemical analysis of three selected plant species, namely, Spathodea campanulata, Talipariti tiliaceus and Xylocarpus granatum, most of the selected plants have no or low amount of flavonoid and alkaloid, and all the leaves samples are rich in phenolic, saponin and tannin than the flower sample. Then, a total of nine tentative assignments of chemical constituents were detected in the selected plant species through LCMS/MS technique. Each of the chemical compounds acquired has its potential bioactive properties. Overall, the data gained through this study may provide important insights for the banded langur conservation management. Consequently, the information on the bioactive compounds can certainly provide a piece of new knowledge on the usage of the plants, especially for self-medication among primate which may indirectly help in providing a new pathway for the development of useful drugs in the medicinal world. 2021-09 Thesis http://eprints.uthm.edu.my/6435/ http://eprints.uthm.edu.my/6435/1/24p%20NORHIDAYAH%20HARIS.pdf text en public http://eprints.uthm.edu.my/6435/2/NORHIDAYAH%20HARIS%20COPYRIGHT%20DECLARATION.pdf text en staffonly http://eprints.uthm.edu.my/6435/3/NORHIDAYAH%20HARIS%20WATERMARK.pdf text en validuser mphil masters Universiti Tun Hussein Malaysia Fakulti Sains Gunaan dan Teknologi |
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Universiti Tun Hussein Onn Malaysia |
| collection |
UTHM Institutional Repository |
| language |
English English English |
| topic |
QC Physics T Technology (General) |
| spellingShingle |
QC Physics T Technology (General) Haris, Norhidayah DNA metabarcoding and phytochemical analysis of plants consumed by banded langur (presbytis femoralis) in Johor |
| description |
Banded langur (Presbytis femoralis) is a leaf-eating monkey that is now classified as
vulnerable in IUCN Red List and to date, there are less than 500 individuals of P.
femoralis that can be observed in some fragmented areas in Johor. Currently, banded
langur is facing a problem in which there is no comprehensive record of their plants’
selection and there is no information on phytochemical compositions in their diets.
This data is crucial for their conservation plan along with solving the human-wildlife
conflict, but it is very challenging to identify the plants consumed by them through
direct observations. Thus, the objectives of this study are to investigate the plant
selection by P. femoralis based on a DNA metabarcoding approach using the trnL
region of chloroplast DNA and to identify the phytochemical compounds in the
selected plant species consumed for bioprospecting purposes. Through the sequencing
method of four faecal samples collected, a total of 140611 operational taxonomic units
(OTUs) were detected which were assigned to 38 families and 71 genera of plants. The
plants from the family of Fabaceae (3.03%) contributed to the most plant food
consumed, followed by the family of Euphorbiaceae (2.63%) and Malvaceae (1.10%).
As for the phytochemical analysis of three selected plant species, namely, Spathodea
campanulata, Talipariti tiliaceus and Xylocarpus granatum, most of the selected
plants have no or low amount of flavonoid and alkaloid, and all the leaves samples are
rich in phenolic, saponin and tannin than the flower sample. Then, a total of nine
tentative assignments of chemical constituents were detected in the selected plant
species through LCMS/MS technique. Each of the chemical compounds acquired has
its potential bioactive properties. Overall, the data gained through this study may
provide important insights for the banded langur conservation management.
Consequently, the information on the bioactive compounds can certainly provide a
piece of new knowledge on the usage of the plants, especially for self-medication
among primate which may indirectly help in providing a new pathway for the
development of useful drugs in the medicinal world. |
| format |
Thesis |
| qualification_name |
Master of Philosophy (M.Phil.) |
| qualification_level |
Master's degree |
| author |
Haris, Norhidayah |
| author_facet |
Haris, Norhidayah |
| author_sort |
Haris, Norhidayah |
| title |
DNA metabarcoding and phytochemical analysis of plants consumed by banded langur (presbytis femoralis) in Johor |
| title_short |
DNA metabarcoding and phytochemical analysis of plants consumed by banded langur (presbytis femoralis) in Johor |
| title_full |
DNA metabarcoding and phytochemical analysis of plants consumed by banded langur (presbytis femoralis) in Johor |
| title_fullStr |
DNA metabarcoding and phytochemical analysis of plants consumed by banded langur (presbytis femoralis) in Johor |
| title_full_unstemmed |
DNA metabarcoding and phytochemical analysis of plants consumed by banded langur (presbytis femoralis) in Johor |
| title_sort |
dna metabarcoding and phytochemical analysis of plants consumed by banded langur (presbytis femoralis) in johor |
| granting_institution |
Universiti Tun Hussein Malaysia |
| granting_department |
Fakulti Sains Gunaan dan Teknologi |
| publishDate |
2021 |
| url |
http://eprints.uthm.edu.my/6435/1/24p%20NORHIDAYAH%20HARIS.pdf http://eprints.uthm.edu.my/6435/2/NORHIDAYAH%20HARIS%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/6435/3/NORHIDAYAH%20HARIS%20WATERMARK.pdf |
| _version_ |
1747831062267953152 |