Oil palm residue biodelignification by bacillus cereus ligninase from coptotermus curvignathus gut for handsheet production
Conventional methods used to delignify lignin using mechanical and chemical process consumed high energy and environmentally unfriendly. Meanwhile, the demand for paper substantially increased with declined wood fibres. In this study, Bacillus cereus isolated from termite species Coptotermes curv...
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my-uthm-ep.65042022-02-06T21:25:22Z Oil palm residue biodelignification by bacillus cereus ligninase from coptotermus curvignathus gut for handsheet production 2020-11 Mutia Syarifah, Sharfina T Technology (General) TS1080-1268 Paper manufacture and trade Conventional methods used to delignify lignin using mechanical and chemical process consumed high energy and environmentally unfriendly. Meanwhile, the demand for paper substantially increased with declined wood fibres. In this study, Bacillus cereus isolated from termite species Coptotermes curvignathus gut’s bacteria was tested for biodelignification capability on palm oil biomasses: oil palm leaves (OPL), oil palm trunk (OPT) and empty fruit bunch (EFB). The biopulp was produced under submerged fermentation (SmF) using Luria Bertani (LB) broth at pH 6.5, 120 rpm and 37 °C for 7 days. The enzyme expression was partially characterized and protein sequenced. There were 11 enzymes responsible for lignin degradation such as 4aminobutyrate aminotransferase (GABA), serine hydrolase, amidohydrolase, chemotaxis protein, GMC family protein, glycosyltransferase, heme peroxidase, phosphate binding protein PstS, ABC transporter ATP-binding protein, nitrate reductase, and nitrite reductase. The delignification ability was tested on its chemical composition. Methods used followed technical association pulp and paper industry TAPPI T 222 om-02 (lignin content), Kurscher-Hoffner (holocellulose and hemicellulose content) and Chlorite (cellulose content). The highest lignin reduction was 21.7% for treated EFB followed by OPT (7.0%) and OPL (9.2%). EFB showed highest reduction (76.9%) for scanning electron microscope (SEM) and imageJ analysis and lowest gap average area (0.03 mm ) compared with untreated OPL which were 26.3% and 1.63 mm 2 , respectively. The treated biopulps were ground using refiner mechanical pulping (RMP) and proceeded handsheet making according to technical association pulp and paper industry (TAPPI) standard methods. Treated EFB performed best in mechanical properties with higher tensile index (7.15 Nm/g), tearing index (1.69 mN.m 2 /g) which supports paper strength with fixed grammage and thickness value. This concludes that Bacillus cereus delignification on EFB sample is the finest pair for handsheet production. This research proposed an alternative biopulping process on oil palm residues handsheets. 2 /g) and bursting index (0.35 kPa.m 2 2020-11 Thesis http://eprints.uthm.edu.my/6504/ http://eprints.uthm.edu.my/6504/1/24p%20SHARFINA%20MUTIA%20SYARIFAH.pdf text en public http://eprints.uthm.edu.my/6504/2/SHARFINA%20MUTIA%20SYARIFAH%20COPYRIGHT%20DECLARATION.pdf text en staffonly http://eprints.uthm.edu.my/6504/3/SHARFINA%20MUTIA%20SYARIFAH%20WATERMARK.pdf text en validuser other masters Universiti Tun Hussein Malaysia Fakulti Teknologi Kejuruteraan |
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Universiti Tun Hussein Onn Malaysia |
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UTHM Institutional Repository |
| language |
English English English |
| topic |
T Technology (General) TS1080-1268 Paper manufacture and trade |
| spellingShingle |
T Technology (General) TS1080-1268 Paper manufacture and trade Mutia Syarifah, Sharfina Oil palm residue biodelignification by bacillus cereus ligninase from coptotermus curvignathus gut for handsheet production |
| description |
Conventional methods used to delignify lignin using mechanical and chemical process
consumed high energy and environmentally unfriendly. Meanwhile, the demand for
paper substantially increased with declined wood fibres. In this study, Bacillus cereus
isolated from termite species Coptotermes curvignathus gut’s bacteria was tested for
biodelignification capability on palm oil biomasses: oil palm leaves (OPL), oil palm
trunk (OPT) and empty fruit bunch (EFB). The biopulp was produced under
submerged fermentation (SmF) using Luria Bertani (LB) broth at pH 6.5, 120 rpm and
37 °C for 7 days. The enzyme expression was partially characterized and protein
sequenced. There were 11 enzymes responsible for lignin degradation such as 4aminobutyrate
aminotransferase (GABA), serine hydrolase, amidohydrolase,
chemotaxis protein, GMC family protein, glycosyltransferase, heme peroxidase,
phosphate binding protein PstS, ABC transporter ATP-binding protein, nitrate
reductase, and nitrite reductase. The delignification ability was tested on its chemical
composition. Methods used followed technical association pulp and paper industry
TAPPI T 222 om-02 (lignin content), Kurscher-Hoffner (holocellulose and
hemicellulose content) and Chlorite (cellulose content). The highest lignin reduction
was 21.7% for treated EFB followed by OPT (7.0%) and OPL (9.2%). EFB showed
highest reduction (76.9%) for scanning electron microscope (SEM) and imageJ
analysis and lowest gap average area (0.03 mm
) compared with untreated OPL which
were 26.3% and 1.63 mm
2
, respectively. The treated biopulps were ground using
refiner mechanical pulping (RMP) and proceeded handsheet making according to
technical association pulp and paper industry (TAPPI) standard methods. Treated EFB
performed best in mechanical properties with higher tensile index (7.15 Nm/g), tearing
index (1.69 mN.m
2
/g) which supports paper
strength with fixed grammage and thickness value. This concludes that Bacillus cereus
delignification on EFB sample is the finest pair for handsheet production. This
research proposed an alternative biopulping process on oil palm residues handsheets.
2
/g) and bursting index (0.35 kPa.m
2 |
| format |
Thesis |
| qualification_name |
other |
| qualification_level |
Master's degree |
| author |
Mutia Syarifah, Sharfina |
| author_facet |
Mutia Syarifah, Sharfina |
| author_sort |
Mutia Syarifah, Sharfina |
| title |
Oil palm residue biodelignification by bacillus cereus ligninase from coptotermus curvignathus gut for handsheet production |
| title_short |
Oil palm residue biodelignification by bacillus cereus ligninase from coptotermus curvignathus gut for handsheet production |
| title_full |
Oil palm residue biodelignification by bacillus cereus ligninase from coptotermus curvignathus gut for handsheet production |
| title_fullStr |
Oil palm residue biodelignification by bacillus cereus ligninase from coptotermus curvignathus gut for handsheet production |
| title_full_unstemmed |
Oil palm residue biodelignification by bacillus cereus ligninase from coptotermus curvignathus gut for handsheet production |
| title_sort |
oil palm residue biodelignification by bacillus cereus ligninase from coptotermus curvignathus gut for handsheet production |
| granting_institution |
Universiti Tun Hussein Malaysia |
| granting_department |
Fakulti Teknologi Kejuruteraan |
| publishDate |
2020 |
| url |
http://eprints.uthm.edu.my/6504/1/24p%20SHARFINA%20MUTIA%20SYARIFAH.pdf http://eprints.uthm.edu.my/6504/2/SHARFINA%20MUTIA%20SYARIFAH%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/6504/3/SHARFINA%20MUTIA%20SYARIFAH%20WATERMARK.pdf |
| _version_ |
1747831075009200128 |