Investigation of the physical properties of cholesteryl ester liquid crystal and the interaction with cells

Cholesteryl ester liquid crystals (CELC) were demonstrated with application in biosensing and microtissue regeneration. The affinity of the cells to this liquid crystal is unclear and required further investigation. This study focused on characterising the physical properties of CELC and interaction...

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Main Author:	Wan Omar, Wan Ibtisam
Format:	Thesis
Language:	English English
Published:	2016
Subjects:	QH573-671 Cytology
Online Access:	http://eprints.uthm.edu.my/737/1/24p%20WAN%20IBTISAM%20WAN%20OMAR.pdf http://eprints.uthm.edu.my/737/2/WAN%20IBTISAM%20WAN%20OMAR%20WATERMARK.pdf
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spelling	my-uthm-ep.7372021-08-30T07:32:37Z Investigation of the physical properties of cholesteryl ester liquid crystal and the interaction with cells 2016-01 Wan Omar, Wan Ibtisam QH573-671 Cytology Cholesteryl ester liquid crystals (CELC) were demonstrated with application in biosensing and microtissue regeneration. The affinity of the cells to this liquid crystal is unclear and required further investigation. This study focused on characterising the physical properties of CELC and interaction of human keratinocytes with CELC. The physical properties of CELC were characterised by a custom built contact angle measurement system and bubble pressure measurement apparatus. Other methods such as pendant drop were applied to determine the critical surface tension of the CELC. Then, the characterization of the CELC was continued by using Differential Scanning Calorimeter (DSC), X-ray Diffraction (XRD), Polarising Microscopy (POM) and Fourier Transform Infrared Spectroscopy (FTIR). Nonetheless, the morphology of cells interaction with CELC after it reached confluency was studied using Field Emission Scanning Electron Microscopy (FESEM) and non-contact mode of Atomic Force Microscopy (AFM). The results showed that the critical surface tension of the liquid crystal using contact angle was 37.5 mN/m and the surface tension measured using pendant drop method was found to be 23.6 mN/m. Both results indicate that the surface of the liquid crystal was moderately hydrophobic. From the DSC, CELC was found stable at room and incubator temperature. From XRD results, the compound of CELC interacts in cell culture media self-assembles into lyotropic layer. POM and FTIR analysis showed CELC after immersion in media displayed lyotropic smectic phases. The AFM and FESEM images indicated good adhesion of cells on the CELC. This research thus showed that the hydrophilic layers of lyotropic phase of cholesteryl ester liquid crystal were demonstrated with biophysical properties that support the adhesion of cells. 2016-01 Thesis http://eprints.uthm.edu.my/737/ http://eprints.uthm.edu.my/737/1/24p%20WAN%20IBTISAM%20WAN%20OMAR.pdf text en public http://eprints.uthm.edu.my/737/2/WAN%20IBTISAM%20WAN%20OMAR%20WATERMARK.pdf text en validuser mphil masters Universiti Tun Hussein Onn Malaysia Faculty of Electrical and Electronic Engineering
institution	Universiti Tun Hussein Onn Malaysia
collection	UTHM Institutional Repository
language	English English
topic	QH573-671 Cytology
spellingShingle	QH573-671 Cytology Wan Omar, Wan Ibtisam Investigation of the physical properties of cholesteryl ester liquid crystal and the interaction with cells
description	Cholesteryl ester liquid crystals (CELC) were demonstrated with application in biosensing and microtissue regeneration. The affinity of the cells to this liquid crystal is unclear and required further investigation. This study focused on characterising the physical properties of CELC and interaction of human keratinocytes with CELC. The physical properties of CELC were characterised by a custom built contact angle measurement system and bubble pressure measurement apparatus. Other methods such as pendant drop were applied to determine the critical surface tension of the CELC. Then, the characterization of the CELC was continued by using Differential Scanning Calorimeter (DSC), X-ray Diffraction (XRD), Polarising Microscopy (POM) and Fourier Transform Infrared Spectroscopy (FTIR). Nonetheless, the morphology of cells interaction with CELC after it reached confluency was studied using Field Emission Scanning Electron Microscopy (FESEM) and non-contact mode of Atomic Force Microscopy (AFM). The results showed that the critical surface tension of the liquid crystal using contact angle was 37.5 mN/m and the surface tension measured using pendant drop method was found to be 23.6 mN/m. Both results indicate that the surface of the liquid crystal was moderately hydrophobic. From the DSC, CELC was found stable at room and incubator temperature. From XRD results, the compound of CELC interacts in cell culture media self-assembles into lyotropic layer. POM and FTIR analysis showed CELC after immersion in media displayed lyotropic smectic phases. The AFM and FESEM images indicated good adhesion of cells on the CELC. This research thus showed that the hydrophilic layers of lyotropic phase of cholesteryl ester liquid crystal were demonstrated with biophysical properties that support the adhesion of cells.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Wan Omar, Wan Ibtisam
author_facet	Wan Omar, Wan Ibtisam
author_sort	Wan Omar, Wan Ibtisam
title	Investigation of the physical properties of cholesteryl ester liquid crystal and the interaction with cells
title_short	Investigation of the physical properties of cholesteryl ester liquid crystal and the interaction with cells
title_full	Investigation of the physical properties of cholesteryl ester liquid crystal and the interaction with cells
title_fullStr	Investigation of the physical properties of cholesteryl ester liquid crystal and the interaction with cells
title_full_unstemmed	Investigation of the physical properties of cholesteryl ester liquid crystal and the interaction with cells
title_sort	investigation of the physical properties of cholesteryl ester liquid crystal and the interaction with cells
granting_institution	Universiti Tun Hussein Onn Malaysia
granting_department	Faculty of Electrical and Electronic Engineering
publishDate	2016
url	http://eprints.uthm.edu.my/737/1/24p%20WAN%20IBTISAM%20WAN%20OMAR.pdf http://eprints.uthm.edu.my/737/2/WAN%20IBTISAM%20WAN%20OMAR%20WATERMARK.pdf
_version_	1747830670563999744

Investigation of the physical properties of cholesteryl ester liquid crystal and the interaction with cells

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