Development of an electronic aerosol atomisation system for generating three-dimensional (3D) cells in microencapsulations and microtissues characterisation

Cell encapsulation is a micro technology widely applied in cell and tissue engineering, tissue transplantation and regenerative medicine. Various techniques had been developed for microencapsulation of cells but these techniques presented threat to the cells due to the harsh or chemical treatment ap...

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Main Author:	Leong, Wai Yean
Format:	Thesis
Language:	English English
Published:	2016
Subjects:	RS Pharmacy and materia medica
Online Access:	http://eprints.uthm.edu.my/768/1/24p%20LEONG%20WAI%20YEAN.pdf http://eprints.uthm.edu.my/768/2/LEONG%20WAI%20YEAN%20WATERMARK.pdf
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spelling	my-uthm-ep.7682021-08-30T07:52:03Z Development of an electronic aerosol atomisation system for generating three-dimensional (3D) cells in microencapsulations and microtissues characterisation 2016-12 Leong, Wai Yean RS Pharmacy and materia medica Cell encapsulation is a micro technology widely applied in cell and tissue engineering, tissue transplantation and regenerative medicine. Various techniques had been developed for microencapsulation of cells but these techniques presented threat to the cells due to the harsh or chemical treatment applied. In this research, a simple and economic electronic aerosol atomisation system was proposed for producing calcium alginate microcapsules. The system was developed with the incorporation of a conventional syringe pump, a customised air pump and motor controller circuits. The microcapsules and 3D microtissues were biophysically characterised. For the output of the system, the microcapsules size slightly increased with the extrusion rates and decreased significantly with the airflow rates. At an extrusion rate of 20 μl/min and airflow rate of 0.3 l/min, microcapsules with a diameter ranging from 220 - 270 μm were generated. The polymerisation time for the microcapsules was approximately 10 minutes after the immersion in calcium chloride solutions. The microcapsules showed high porous surface structure in field emission-scanning electron microscopy (FE-SEM) imaging. Keratinocytes (HaCaT) and Oral Squamous Cell Carcinoma (ORL-48) cells at cell densities of 3 × 107 and 9 × 107 cells/ml, respectively were applied for encapsulation and successfully grew into microtissues after 16 days of culture. The fourier transform infrared (FTIR) spectroscopy of the 3D cells showed stretching in phosphate bond of Deoxyribonucleic acid (DNA) and Ribonucleic acid (RNA) backbone, lipid and protein. The cells of HaCaT and ORL-48 microtissues were viable and they were characterised by different nucleus size. Replating experiment demonstrated that the cells in the microtissues could spread and proliferate in the culture dish. The electronic aerosol atomisation system developed in this work has successfully produced microcapsules with controllable size and applicable for growing microtissues. The microtissues produced are potentially a useful cell model for the study of cytochemicals. 2016-12 Thesis http://eprints.uthm.edu.my/768/ http://eprints.uthm.edu.my/768/1/24p%20LEONG%20WAI%20YEAN.pdf text en public http://eprints.uthm.edu.my/768/2/LEONG%20WAI%20YEAN%20WATERMARK.pdf text en validuser mphil masters Universiti Tun Hussein Onn Malaysia Fakulti Kejuruteraan Elektrik dan Elektronik
institution	Universiti Tun Hussein Onn Malaysia
collection	UTHM Institutional Repository
language	English English
topic	RS Pharmacy and materia medica
spellingShingle	RS Pharmacy and materia medica Leong, Wai Yean Development of an electronic aerosol atomisation system for generating three-dimensional (3D) cells in microencapsulations and microtissues characterisation
description	Cell encapsulation is a micro technology widely applied in cell and tissue engineering, tissue transplantation and regenerative medicine. Various techniques had been developed for microencapsulation of cells but these techniques presented threat to the cells due to the harsh or chemical treatment applied. In this research, a simple and economic electronic aerosol atomisation system was proposed for producing calcium alginate microcapsules. The system was developed with the incorporation of a conventional syringe pump, a customised air pump and motor controller circuits. The microcapsules and 3D microtissues were biophysically characterised. For the output of the system, the microcapsules size slightly increased with the extrusion rates and decreased significantly with the airflow rates. At an extrusion rate of 20 μl/min and airflow rate of 0.3 l/min, microcapsules with a diameter ranging from 220 - 270 μm were generated. The polymerisation time for the microcapsules was approximately 10 minutes after the immersion in calcium chloride solutions. The microcapsules showed high porous surface structure in field emission-scanning electron microscopy (FE-SEM) imaging. Keratinocytes (HaCaT) and Oral Squamous Cell Carcinoma (ORL-48) cells at cell densities of 3 × 107 and 9 × 107 cells/ml, respectively were applied for encapsulation and successfully grew into microtissues after 16 days of culture. The fourier transform infrared (FTIR) spectroscopy of the 3D cells showed stretching in phosphate bond of Deoxyribonucleic acid (DNA) and Ribonucleic acid (RNA) backbone, lipid and protein. The cells of HaCaT and ORL-48 microtissues were viable and they were characterised by different nucleus size. Replating experiment demonstrated that the cells in the microtissues could spread and proliferate in the culture dish. The electronic aerosol atomisation system developed in this work has successfully produced microcapsules with controllable size and applicable for growing microtissues. The microtissues produced are potentially a useful cell model for the study of cytochemicals.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Leong, Wai Yean
author_facet	Leong, Wai Yean
author_sort	Leong, Wai Yean
title	Development of an electronic aerosol atomisation system for generating three-dimensional (3D) cells in microencapsulations and microtissues characterisation
title_short	Development of an electronic aerosol atomisation system for generating three-dimensional (3D) cells in microencapsulations and microtissues characterisation
title_full	Development of an electronic aerosol atomisation system for generating three-dimensional (3D) cells in microencapsulations and microtissues characterisation
title_fullStr	Development of an electronic aerosol atomisation system for generating three-dimensional (3D) cells in microencapsulations and microtissues characterisation
title_full_unstemmed	Development of an electronic aerosol atomisation system for generating three-dimensional (3D) cells in microencapsulations and microtissues characterisation
title_sort	development of an electronic aerosol atomisation system for generating three-dimensional (3d) cells in microencapsulations and microtissues characterisation
granting_institution	Universiti Tun Hussein Onn Malaysia
granting_department	Fakulti Kejuruteraan Elektrik dan Elektronik
publishDate	2016
url	http://eprints.uthm.edu.my/768/1/24p%20LEONG%20WAI%20YEAN.pdf http://eprints.uthm.edu.my/768/2/LEONG%20WAI%20YEAN%20WATERMARK.pdf
_version_	1747830677272788992

Development of an electronic aerosol atomisation system for generating three-dimensional (3D) cells in microencapsulations and microtissues characterisation

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