Effect of salt stress on the physiological responses, phytochemicals and antioxidant activities of clitoria ternatea var. Pleniflora

Soil salinity is a process of increasing salt in soils. It has become a problem since it keeps spreading geographically from coastal areas to other fertile lands. Therefore, many plants have developed some series of adaptations to mitigate against salinity. Salinity limits the vegetative producti...

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Main Author: Mohd Nasim, Nur A’in
Format: Thesis
Language:English
English
English
Published: 2021
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spelling my-uthm-ep.82952023-02-07T03:45:16Z Effect of salt stress on the physiological responses, phytochemicals and antioxidant activities of clitoria ternatea var. Pleniflora 2021-12 Mohd Nasim, Nur A’in T Technology (General) Soil salinity is a process of increasing salt in soils. It has become a problem since it keeps spreading geographically from coastal areas to other fertile lands. Therefore, many plants have developed some series of adaptations to mitigate against salinity. Salinity limits the vegetative productions and changes the ecological health of estuaries due to unproductive lands. It lowers the crop yields for daily purposes as well as a threat for biodiversity in which living things loses habitat for both land and in water. Thus, the purpose of this study was to evaluate the growth traits of Clitoria ternatea var. plenifora exposed to different concentrations of sodium chloride, NaCl. Accordingly, it also brings insights into the effect of various concentrations of salt stress on the qualitative phytochemicals content of tannins, flavonoids, glycosides, steroids, terpenoids, alkaloids, phenols and saponins. Moreover, the total phenolic and flavonoid contents were determined quantitatively. Ferric reducing antioxidant power (FRAP), 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2- diphenyl-1-picrylhydrazyl (DPPH) assays were conducted to evaluate the antioxidant activities of the Clitoria ternatea var. plenifora. Thus, the results from this study revealed that all of the test growth traits ̶ the number of branches and leaves, height of plants, fresh weight of leaves and dry weight of stem, leaves and roots ̶ were the highest under 200 mM of NaCl treatment. Apart from that, under 200 mM of NaCl, Clitoria ternatea var. pleniflora was shown to contain the highest total of phenolic and flavonoid contents among of all the salt stress treatments. Thereupon, the antioxidant activities of FRAP, DPPH and ABTS were also showed to reach at their highest activities under 200 mM of NaCl. In conclusion, 200 mM of NaCl is the optimum concentration for Clitoria ternatea var. pleniflora to improve the growth traits, phytochemicals and antioxidants of Clitoria ternatea var. pleniflora. 2021-12 Thesis http://eprints.uthm.edu.my/8295/ http://eprints.uthm.edu.my/8295/1/24p%20NUR%20A%27IN%20MOHD%20NASIM.pdf text en public http://eprints.uthm.edu.my/8295/2/NUR%20A%27IN%20MOHD%20NASIM%20COPYRIGHT%20DECLARATION.pdf text en public http://eprints.uthm.edu.my/8295/3/NUR%20A%27IN%20MOHD%20NASIM%20WATERMARK.pdf text en validuser mphil masters Universiti Tun Hussein Onn Malaysia Fakulti Sains Gunaan dan Teknologi
institution Universiti Tun Hussein Onn Malaysia
collection UTHM Institutional Repository
language English
English
English
topic T Technology (General)
spellingShingle T Technology (General)
Mohd Nasim, Nur A’in
Effect of salt stress on the physiological responses, phytochemicals and antioxidant activities of clitoria ternatea var. Pleniflora
description Soil salinity is a process of increasing salt in soils. It has become a problem since it keeps spreading geographically from coastal areas to other fertile lands. Therefore, many plants have developed some series of adaptations to mitigate against salinity. Salinity limits the vegetative productions and changes the ecological health of estuaries due to unproductive lands. It lowers the crop yields for daily purposes as well as a threat for biodiversity in which living things loses habitat for both land and in water. Thus, the purpose of this study was to evaluate the growth traits of Clitoria ternatea var. plenifora exposed to different concentrations of sodium chloride, NaCl. Accordingly, it also brings insights into the effect of various concentrations of salt stress on the qualitative phytochemicals content of tannins, flavonoids, glycosides, steroids, terpenoids, alkaloids, phenols and saponins. Moreover, the total phenolic and flavonoid contents were determined quantitatively. Ferric reducing antioxidant power (FRAP), 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2- diphenyl-1-picrylhydrazyl (DPPH) assays were conducted to evaluate the antioxidant activities of the Clitoria ternatea var. plenifora. Thus, the results from this study revealed that all of the test growth traits ̶ the number of branches and leaves, height of plants, fresh weight of leaves and dry weight of stem, leaves and roots ̶ were the highest under 200 mM of NaCl treatment. Apart from that, under 200 mM of NaCl, Clitoria ternatea var. pleniflora was shown to contain the highest total of phenolic and flavonoid contents among of all the salt stress treatments. Thereupon, the antioxidant activities of FRAP, DPPH and ABTS were also showed to reach at their highest activities under 200 mM of NaCl. In conclusion, 200 mM of NaCl is the optimum concentration for Clitoria ternatea var. pleniflora to improve the growth traits, phytochemicals and antioxidants of Clitoria ternatea var. pleniflora.
format Thesis
qualification_name Master of Philosophy (M.Phil.)
qualification_level Master's degree
author Mohd Nasim, Nur A’in
author_facet Mohd Nasim, Nur A’in
author_sort Mohd Nasim, Nur A’in
title Effect of salt stress on the physiological responses, phytochemicals and antioxidant activities of clitoria ternatea var. Pleniflora
title_short Effect of salt stress on the physiological responses, phytochemicals and antioxidant activities of clitoria ternatea var. Pleniflora
title_full Effect of salt stress on the physiological responses, phytochemicals and antioxidant activities of clitoria ternatea var. Pleniflora
title_fullStr Effect of salt stress on the physiological responses, phytochemicals and antioxidant activities of clitoria ternatea var. Pleniflora
title_full_unstemmed Effect of salt stress on the physiological responses, phytochemicals and antioxidant activities of clitoria ternatea var. Pleniflora
title_sort effect of salt stress on the physiological responses, phytochemicals and antioxidant activities of clitoria ternatea var. pleniflora
granting_institution Universiti Tun Hussein Onn Malaysia
granting_department Fakulti Sains Gunaan dan Teknologi
publishDate 2021
url http://eprints.uthm.edu.my/8295/1/24p%20NUR%20A%27IN%20MOHD%20NASIM.pdf
http://eprints.uthm.edu.my/8295/2/NUR%20A%27IN%20MOHD%20NASIM%20COPYRIGHT%20DECLARATION.pdf
http://eprints.uthm.edu.my/8295/3/NUR%20A%27IN%20MOHD%20NASIM%20WATERMARK.pdf
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