Identification of polystyrene degrading bacteria and potential enzyme action from zophobas morio for improvement and sustainable biodegradation of waste

Polystyrene (PS) is a widely used petroleum-based plastic polymer. However, it is recalcitrant towards degradation and expected to remain longer in the environment which leads to a major waste problem. Moreover, conventional PS disposal and waste management consume massive amounts of chemicals and e...

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Main Author:	Tan, Kian Meng
Format:	Thesis
Language:	English English English
Published:	2021
Subjects:	T Technology (General)
Online Access:	http://eprints.uthm.edu.my/8356/1/24p%20TAN%20KIAN%20MENG.pdf http://eprints.uthm.edu.my/8356/2/TAN%20KIAN%20MENG%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/8356/3/TAN%20KIAN%20MENG%20WATERMARK.pdf
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spelling	my-uthm-ep.83562023-02-28T01:22:14Z Identification of polystyrene degrading bacteria and potential enzyme action from zophobas morio for improvement and sustainable biodegradation of waste 2021-10 Tan, Kian Meng T Technology (General) Polystyrene (PS) is a widely used petroleum-based plastic polymer. However, it is recalcitrant towards degradation and expected to remain longer in the environment which leads to a major waste problem. Moreover, conventional PS disposal and waste management consume massive amounts of chemicals and energy which have deleterious effects on the environment. Therefore, the aim of this research is to explore an alternative approach for PS biodegradation by identifying PS-degrading bacteria and the potential enzyme action isolated from the gut of PS feeding insect, Zophobas morio. The bacteria that capable of degrading PS was isolated and presumed identified and characterised as Bacillus megaterium. The viability and metabolic activity of Bacillus megaterium to utilise PS as sole carbon source were validated through indicator redox probe 2,3,5-triphenyltetrazolium chloride. Biodegradability of Bacillus megaterium corroborated by biofilm quantification, FESEM and FTIR analysis. Biofilm formed and morphological changes induced by degradation were clearly viewed via FESEM and the chemical bonds alteration was detected by FTIR analysis. The expressed PDE were profiled with SDS-PAGE and the three most visible bands were obtained at 20, 45 and 60 kDa. The protein sequencing results revealed the 20 kDa protein (Isochorismatase), 45 and 60 kDa (insignificant matches). The de novo sequencing of 60 kDa protein showed similarity to trypsin (Bos Taurus), whilst BLAST results revealed that it is a serine hydrolase enzyme. In summary, the 60 kDa protein is a multi-domain protein, with one domain functioning identified as a hydrolase. The whole protein is a hypothetical protein, with no annotated function. Thus, the results revealed unidentified, perhaps novel protein. Furthermore, PS enzymatic degraded products were identified through GC/MS analysis as part of the complementary study. The conversion pathways of PS to various aromatic and non-aromatic compounds were also proposed. The findings of this research provide insight into PS biodegradation and its potential application in waste management and polymer industries, thus encouraging the use of green and sustainable technology. 2021-10 Thesis http://eprints.uthm.edu.my/8356/ http://eprints.uthm.edu.my/8356/1/24p%20TAN%20KIAN%20MENG.pdf text en public http://eprints.uthm.edu.my/8356/2/TAN%20KIAN%20MENG%20COPYRIGHT%20DECLARATION.pdf text en staffonly http://eprints.uthm.edu.my/8356/3/TAN%20KIAN%20MENG%20WATERMARK.pdf text en validuser mphil doctoral Universiti Tun Hussein Onn Malaysia Fakulti Teknologi Kejuruteraan
institution	Universiti Tun Hussein Onn Malaysia
collection	UTHM Institutional Repository
language	English English English
topic	T Technology (General)
spellingShingle	T Technology (General) Tan, Kian Meng Identification of polystyrene degrading bacteria and potential enzyme action from zophobas morio for improvement and sustainable biodegradation of waste
description	Polystyrene (PS) is a widely used petroleum-based plastic polymer. However, it is recalcitrant towards degradation and expected to remain longer in the environment which leads to a major waste problem. Moreover, conventional PS disposal and waste management consume massive amounts of chemicals and energy which have deleterious effects on the environment. Therefore, the aim of this research is to explore an alternative approach for PS biodegradation by identifying PS-degrading bacteria and the potential enzyme action isolated from the gut of PS feeding insect, Zophobas morio. The bacteria that capable of degrading PS was isolated and presumed identified and characterised as Bacillus megaterium. The viability and metabolic activity of Bacillus megaterium to utilise PS as sole carbon source were validated through indicator redox probe 2,3,5-triphenyltetrazolium chloride. Biodegradability of Bacillus megaterium corroborated by biofilm quantification, FESEM and FTIR analysis. Biofilm formed and morphological changes induced by degradation were clearly viewed via FESEM and the chemical bonds alteration was detected by FTIR analysis. The expressed PDE were profiled with SDS-PAGE and the three most visible bands were obtained at 20, 45 and 60 kDa. The protein sequencing results revealed the 20 kDa protein (Isochorismatase), 45 and 60 kDa (insignificant matches). The de novo sequencing of 60 kDa protein showed similarity to trypsin (Bos Taurus), whilst BLAST results revealed that it is a serine hydrolase enzyme. In summary, the 60 kDa protein is a multi-domain protein, with one domain functioning identified as a hydrolase. The whole protein is a hypothetical protein, with no annotated function. Thus, the results revealed unidentified, perhaps novel protein. Furthermore, PS enzymatic degraded products were identified through GC/MS analysis as part of the complementary study. The conversion pathways of PS to various aromatic and non-aromatic compounds were also proposed. The findings of this research provide insight into PS biodegradation and its potential application in waste management and polymer industries, thus encouraging the use of green and sustainable technology.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Doctorate
author	Tan, Kian Meng
author_facet	Tan, Kian Meng
author_sort	Tan, Kian Meng
title	Identification of polystyrene degrading bacteria and potential enzyme action from zophobas morio for improvement and sustainable biodegradation of waste
title_short	Identification of polystyrene degrading bacteria and potential enzyme action from zophobas morio for improvement and sustainable biodegradation of waste
title_full	Identification of polystyrene degrading bacteria and potential enzyme action from zophobas morio for improvement and sustainable biodegradation of waste
title_fullStr	Identification of polystyrene degrading bacteria and potential enzyme action from zophobas morio for improvement and sustainable biodegradation of waste
title_full_unstemmed	Identification of polystyrene degrading bacteria and potential enzyme action from zophobas morio for improvement and sustainable biodegradation of waste
title_sort	identification of polystyrene degrading bacteria and potential enzyme action from zophobas morio for improvement and sustainable biodegradation of waste
granting_institution	Universiti Tun Hussein Onn Malaysia
granting_department	Fakulti Teknologi Kejuruteraan
publishDate	2021
url	http://eprints.uthm.edu.my/8356/1/24p%20TAN%20KIAN%20MENG.pdf http://eprints.uthm.edu.my/8356/2/TAN%20KIAN%20MENG%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/8356/3/TAN%20KIAN%20MENG%20WATERMARK.pdf
_version_	1776103327611748352

Identification of polystyrene degrading bacteria and potential enzyme action from zophobas morio for improvement and sustainable biodegradation of waste

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