In vitro bioactivity of novel chitosan/gelatin/halloysite nanostructured coatings on anodised titanium via electrophoresation for bone implant

In vitro bioactivity of novel chitosan/gelatin/halloysite nanostructured coatings on anodised titanium via electrophoresation for bone implant

Electrophoretic deposition (EPD) involves coating densification via matrix micro/nano-filling on a template-assisted substrate. This mechanical interlock technique has recently been used to avoid coating cracking and delamination. This thesis reports that EPD organic-inorganic nanostructured coating...

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Main Author: Alipal, Janifal
Format: Thesis
Language:English
English
English
Published: 2022
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TP Chemical technology
Online Access:http://eprints.uthm.edu.my/8427/1/24p%20JANIFAL%20ALIPAL.pdf
http://eprints.uthm.edu.my/8427/2/JANIFAL%20ALIPAL%20COPYRIGHT%20DECLARATION.pdf
http://eprints.uthm.edu.my/8427/3/JANIFAL%20ALIPAL%20WATERMARK.pdf
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spelling my-uthm-ep.84272023-02-26T07:22:51Z In vitro bioactivity of novel chitosan/gelatin/halloysite nanostructured coatings on anodised titanium via electrophoresation for bone implant 2022-04 Alipal, Janifal TP Chemical technology Electrophoretic deposition (EPD) involves coating densification via matrix micro/nano-filling on a template-assisted substrate. This mechanical interlock technique has recently been used to avoid coating cracking and delamination. This thesis reports that EPD organic-inorganic nanostructured coatings containing chitosan/gelatin hydrogel and halloysite nanotubes (HNTs) produce an ideal mechanical interlock. In the proposed bio-composite, HNTs are used to densify the coating's mixture. The mechanical interlocking of the proposed bio-composite coating defines its net mechanistic bioactivity. Prior to EPD, the study goes through a substrate pre-processing step in which the cp-Ti surface is modified using micro-arc anodic oxidation (MAO) in a CaP-based electrolyte (a mixture of β-glycerophosphate and calcium acetate). The study discovered that anodised titanium (MAT) in donut-shaped morphology (MAO 350 V) has better mechanical stability and osteogenic cellular response compared to the needle-like structure. The findings determined that the donut-shaped MAT microstructure is the best next choice for the EPD substrate in the coating mechanical interlock study. Despite the fact that the EPD processing parameters were varied (10-30 V; 5-20 min), the mechanically interlock nanostructured coating (template-assisted EPD) significantly improved coating adhesion and osteogenic development in this study. In coating fabrication, the weight fraction of HNTs in the hydrogel is critical, and this study determined that the optimal composition of a steric stabilised organic-inorganic EPD suspension for chitosan/gelatin/HNTs is 6:14:12 g/L. Modifying implant surfaces using novel techniques such as varying substrate morphology and/or degraded coatings has become a popular method for improving implant osseointegration. This recent study established that specific surface features influence how bone cells interact with a material and which specific surface features result in optimal bone integration. In this thesis, MAT is designed to be a highly bioactive EPD substrate, resulting not only in a highly stable coating structure but also in improved osteogenic development, specifically osteoblast mineralisation and differentiation. 2022-04 Thesis http://eprints.uthm.edu.my/8427/ http://eprints.uthm.edu.my/8427/1/24p%20JANIFAL%20ALIPAL.pdf text en public http://eprints.uthm.edu.my/8427/2/JANIFAL%20ALIPAL%20COPYRIGHT%20DECLARATION.pdf text en staffonly http://eprints.uthm.edu.my/8427/3/JANIFAL%20ALIPAL%20WATERMARK.pdf text en validuser phd doctoral Universiti Tun Hussein Onn Malaysia Fakulti Teknologi Kejuruteraan
institution Universiti Tun Hussein Onn Malaysia
collection UTHM Institutional Repository
language English
English
English
topic TP Chemical technology
spellingShingle TP Chemical technology
Alipal, Janifal
In vitro bioactivity of novel chitosan/gelatin/halloysite nanostructured coatings on anodised titanium via electrophoresation for bone implant
description Electrophoretic deposition (EPD) involves coating densification via matrix micro/nano-filling on a template-assisted substrate. This mechanical interlock technique has recently been used to avoid coating cracking and delamination. This thesis reports that EPD organic-inorganic nanostructured coatings containing chitosan/gelatin hydrogel and halloysite nanotubes (HNTs) produce an ideal mechanical interlock. In the proposed bio-composite, HNTs are used to densify the coating's mixture. The mechanical interlocking of the proposed bio-composite coating defines its net mechanistic bioactivity. Prior to EPD, the study goes through a substrate pre-processing step in which the cp-Ti surface is modified using micro-arc anodic oxidation (MAO) in a CaP-based electrolyte (a mixture of β-glycerophosphate and calcium acetate). The study discovered that anodised titanium (MAT) in donut-shaped morphology (MAO 350 V) has better mechanical stability and osteogenic cellular response compared to the needle-like structure. The findings determined that the donut-shaped MAT microstructure is the best next choice for the EPD substrate in the coating mechanical interlock study. Despite the fact that the EPD processing parameters were varied (10-30 V; 5-20 min), the mechanically interlock nanostructured coating (template-assisted EPD) significantly improved coating adhesion and osteogenic development in this study. In coating fabrication, the weight fraction of HNTs in the hydrogel is critical, and this study determined that the optimal composition of a steric stabilised organic-inorganic EPD suspension for chitosan/gelatin/HNTs is 6:14:12 g/L. Modifying implant surfaces using novel techniques such as varying substrate morphology and/or degraded coatings has become a popular method for improving implant osseointegration. This recent study established that specific surface features influence how bone cells interact with a material and which specific surface features result in optimal bone integration. In this thesis, MAT is designed to be a highly bioactive EPD substrate, resulting not only in a highly stable coating structure but also in improved osteogenic development, specifically osteoblast mineralisation and differentiation.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Alipal, Janifal
author_facet Alipal, Janifal
author_sort Alipal, Janifal
title In vitro bioactivity of novel chitosan/gelatin/halloysite nanostructured coatings on anodised titanium via electrophoresation for bone implant
title_short In vitro bioactivity of novel chitosan/gelatin/halloysite nanostructured coatings on anodised titanium via electrophoresation for bone implant
title_full In vitro bioactivity of novel chitosan/gelatin/halloysite nanostructured coatings on anodised titanium via electrophoresation for bone implant
title_fullStr In vitro bioactivity of novel chitosan/gelatin/halloysite nanostructured coatings on anodised titanium via electrophoresation for bone implant
title_full_unstemmed In vitro bioactivity of novel chitosan/gelatin/halloysite nanostructured coatings on anodised titanium via electrophoresation for bone implant
title_sort in vitro bioactivity of novel chitosan/gelatin/halloysite nanostructured coatings on anodised titanium via electrophoresation for bone implant
granting_institution Universiti Tun Hussein Onn Malaysia
granting_department Fakulti Teknologi Kejuruteraan
publishDate 2022
url http://eprints.uthm.edu.my/8427/1/24p%20JANIFAL%20ALIPAL.pdf
http://eprints.uthm.edu.my/8427/2/JANIFAL%20ALIPAL%20COPYRIGHT%20DECLARATION.pdf
http://eprints.uthm.edu.my/8427/3/JANIFAL%20ALIPAL%20WATERMARK.pdf
_version_ 1776103343196733440

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