Documentation, anti-aging activities and phytochemical profiling of selected medicinal plants used by Jakun women in Kampung Peta, Mersing, Johor

Traditional knowledge of indigenous people could become the baseline information for the discovery of anti-aging agent. The objectives of this study were to document the knowledge of Jakun people in Kampung Peta, Mersing, Johor on medicinal plants for women’s healthcare; to investigate the optimal f...

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Main Author:	Ismail, Nur Amalina
Format:	Thesis
Language:	English English English
Published:	2017
Subjects:	R Medicine (General) RS Pharmacy and materia medica
Online Access:	http://eprints.uthm.edu.my/846/1/24p%20NUR%20AMALINA%20ISMAIL.pdf http://eprints.uthm.edu.my/846/2/NUR%20AMALINA%20ISMAIL%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/846/3/NUR%20AMALINA%20ISMAIL%20WATERMARK.pdf
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spelling	my-uthm-ep.8462021-09-06T04:22:27Z Documentation, anti-aging activities and phytochemical profiling of selected medicinal plants used by Jakun women in Kampung Peta, Mersing, Johor 2017-09 Ismail, Nur Amalina R Medicine (General) RS Pharmacy and materia medica Traditional knowledge of indigenous people could become the baseline information for the discovery of anti-aging agent. The objectives of this study were to document the knowledge of Jakun people in Kampung Peta, Mersing, Johor on medicinal plants for women’s healthcare; to investigate the optimal formulations of herbal mixture used by Jakun women based on phytochemicals content and antioxidant activity; to determine the anti-aging potential of the selected formulations; and to investigate the major phytochemical constituents in the formulations. Based on qualitative analysis from semi-structured interview, twelve species of medicinal plants have been documented for women’s healthcare. Among species documented, four species, Cnestis palala (Pengesep), Urceola micrantha (Serapat), Labisia pumila (Kacip fatimah) and Microporus xanthopus (Kulat kelentit kering) that were prepared in the form of mixture have been used for formulation study. About 24 formulations have been developed from the simplex centroid design and tested for total phenolic content (Folin-Ciocalteu method), total flavonoid content (aluminium chloride colorimetric method) and three different antioxidant assays (DPPH scavenging, ABTS decolourization and FRAP assays). Single formulation of Cnestis palala, single formulation of Urceola micrantha and binary mixture of C. palala and U. micrantha are among the optimal formulations with high phytochemicals content and antioxidant activities that were further evaluated for anti-aging activities. For anti-aging activities, five enzymatic assays have been tested on the three formulations which are matrix metalloproteinase-1 (MMP-1) inhibition, elastase inhibition, tyrosinase inhibition, acetyl- and butyrylcholinesterase inhibition assays. Single formulation of U. micrantha showed the highest inhibition towards MMP-1 (49.44 ± 4.11 %) and elastase enzymes (20.33±2.52%), while single formulation of C. palala showed highest inhibitions towards tyrosinase (14.06±0.31%), acetylcholinesterase (32.92±2.13%) and butyrylcholinesterase (34.89±2.84%) enzymes. The identification of phytochemicals compound have been carried out using gas chromatography-mass spectrometer (GC-MS), which showed the presence of 2,2-dimethoxybutane and 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP) in the three formulations extract. The presence of catechol and quinic acid in U. micrantha extract might possibly contribute to anti-aging activities of the extract. These findings could become baseline for the exploration of novel anti-aging agents from natural source by using the traditional knowledge of indigenous people. 2017-09 Thesis http://eprints.uthm.edu.my/846/ http://eprints.uthm.edu.my/846/1/24p%20NUR%20AMALINA%20ISMAIL.pdf text en public http://eprints.uthm.edu.my/846/2/NUR%20AMALINA%20ISMAIL%20COPYRIGHT%20DECLARATION.pdf text en staffonly http://eprints.uthm.edu.my/846/3/NUR%20AMALINA%20ISMAIL%20WATERMARK.pdf text en validuser mphil masters Universiti Tun Hussein Onn Malaysia Fakulti Kejuruteraan Awam dan Alam Bina
institution	Universiti Tun Hussein Onn Malaysia
collection	UTHM Institutional Repository
language	English English English
topic	R Medicine (General) RS Pharmacy and materia medica
spellingShingle	R Medicine (General) RS Pharmacy and materia medica Ismail, Nur Amalina Documentation, anti-aging activities and phytochemical profiling of selected medicinal plants used by Jakun women in Kampung Peta, Mersing, Johor
description	Traditional knowledge of indigenous people could become the baseline information for the discovery of anti-aging agent. The objectives of this study were to document the knowledge of Jakun people in Kampung Peta, Mersing, Johor on medicinal plants for women’s healthcare; to investigate the optimal formulations of herbal mixture used by Jakun women based on phytochemicals content and antioxidant activity; to determine the anti-aging potential of the selected formulations; and to investigate the major phytochemical constituents in the formulations. Based on qualitative analysis from semi-structured interview, twelve species of medicinal plants have been documented for women’s healthcare. Among species documented, four species, Cnestis palala (Pengesep), Urceola micrantha (Serapat), Labisia pumila (Kacip fatimah) and Microporus xanthopus (Kulat kelentit kering) that were prepared in the form of mixture have been used for formulation study. About 24 formulations have been developed from the simplex centroid design and tested for total phenolic content (Folin-Ciocalteu method), total flavonoid content (aluminium chloride colorimetric method) and three different antioxidant assays (DPPH scavenging, ABTS decolourization and FRAP assays). Single formulation of Cnestis palala, single formulation of Urceola micrantha and binary mixture of C. palala and U. micrantha are among the optimal formulations with high phytochemicals content and antioxidant activities that were further evaluated for anti-aging activities. For anti-aging activities, five enzymatic assays have been tested on the three formulations which are matrix metalloproteinase-1 (MMP-1) inhibition, elastase inhibition, tyrosinase inhibition, acetyl- and butyrylcholinesterase inhibition assays. Single formulation of U. micrantha showed the highest inhibition towards MMP-1 (49.44 ± 4.11 %) and elastase enzymes (20.33±2.52%), while single formulation of C. palala showed highest inhibitions towards tyrosinase (14.06±0.31%), acetylcholinesterase (32.92±2.13%) and butyrylcholinesterase (34.89±2.84%) enzymes. The identification of phytochemicals compound have been carried out using gas chromatography-mass spectrometer (GC-MS), which showed the presence of 2,2-dimethoxybutane and 2,3-dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP) in the three formulations extract. The presence of catechol and quinic acid in U. micrantha extract might possibly contribute to anti-aging activities of the extract. These findings could become baseline for the exploration of novel anti-aging agents from natural source by using the traditional knowledge of indigenous people.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Ismail, Nur Amalina
author_facet	Ismail, Nur Amalina
author_sort	Ismail, Nur Amalina
title	Documentation, anti-aging activities and phytochemical profiling of selected medicinal plants used by Jakun women in Kampung Peta, Mersing, Johor
title_short	Documentation, anti-aging activities and phytochemical profiling of selected medicinal plants used by Jakun women in Kampung Peta, Mersing, Johor
title_full	Documentation, anti-aging activities and phytochemical profiling of selected medicinal plants used by Jakun women in Kampung Peta, Mersing, Johor
title_fullStr	Documentation, anti-aging activities and phytochemical profiling of selected medicinal plants used by Jakun women in Kampung Peta, Mersing, Johor
title_full_unstemmed	Documentation, anti-aging activities and phytochemical profiling of selected medicinal plants used by Jakun women in Kampung Peta, Mersing, Johor
title_sort	documentation, anti-aging activities and phytochemical profiling of selected medicinal plants used by jakun women in kampung peta, mersing, johor
granting_institution	Universiti Tun Hussein Onn Malaysia
granting_department	Fakulti Kejuruteraan Awam dan Alam Bina
publishDate	2017
url	http://eprints.uthm.edu.my/846/1/24p%20NUR%20AMALINA%20ISMAIL.pdf http://eprints.uthm.edu.my/846/2/NUR%20AMALINA%20ISMAIL%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/846/3/NUR%20AMALINA%20ISMAIL%20WATERMARK.pdf
_version_	1747830692730896384

Documentation, anti-aging activities and phytochemical profiling of selected medicinal plants used by Jakun women in Kampung Peta, Mersing, Johor

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