Production of biodegradable poly lactic acid from pineapple industry wastewater by lactobacillus casei fermentation

Pineapple processing industry generates large amount of liquid waste, which contains high concentration of biodegradable organic, suspended solid and high biological oxygen demand (BOD), as well as carbohydrates such as glucose and fructose. The by-products effluent can be a substrate for production...

Full description

Saved in:

Bibliographic Details
Main Author:	Hassan, Saber Salem
Format:	Thesis
Language:	English English English
Published:	2017
Subjects:	TD896-899 Industrial and factory wastes
Online Access:	http://eprints.uthm.edu.my/857/2/24p%20SABER%20SALEM%20HASSAN.pdf http://eprints.uthm.edu.my/857/1/SABER%20SALEM%20HASSAN%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/857/3/SABER%20SALEM%20HASSAN%20WATERMARK.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-uthm-ep.857
record_format	uketd_dc
spelling	my-uthm-ep.8572021-09-06T05:41:20Z Production of biodegradable poly lactic acid from pineapple industry wastewater by lactobacillus casei fermentation 2017-08 Hassan, Saber Salem TD896-899 Industrial and factory wastes Pineapple processing industry generates large amount of liquid waste, which contains high concentration of biodegradable organic, suspended solid and high biological oxygen demand (BOD), as well as carbohydrates such as glucose and fructose. The by-products effluent can be a substrate for production of value-added products by microbial fermentation such as biomaterial. However, investigation on the bioconversion of pineapple wastewater to value-added products is still lacking. The objective of this study is to produce biodegradable poly lactic acid (PLA) from pineapple industry waste water by Lactobacillus casei. The L. caseiwas cultivated with a medium containing extracted glucose for 24 h. The optimisation of cultural conditions (temperature, pH, inoculum size, nitrogen source) for lactic acid (LA) production was carried out. Purification of LA by reverse osmosis was done. After that, synthesize of PLA was achieved by direct polymerization of pure LA under a nitrogen atmosphere at an optimum time and temperature. Finally, the characterization of PLA was conducted by gel permeation chromatography (GPC), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). A maximum production of 31.6 g/l LA was obtained at pH 6, 37 °C and 5% (v/v) of inoculum size. Interestingly, the concentration of pure LA was measured up to 54 g/l after purification. The optimum percentage yield of PLA obtained was 10.4% for 160 °C at 12 h and the molecular weight MW was in the range of 1000 Da to 5215 Da. The highly intense band of 2995 was due to CH stretching of the methylene and methyne groups, a range 1716 to 1748 for ester carbonyl group; other significant bands are in region 1118-1360 typical for ester group -CO-O. A clear glass transition (Tg)at 45.3 °C was exhibited when heat synthesis at 160°C. The results obtained confirmed the production of PLA. Hence it can be concluded that pineapple wastewater can be used for PLA production by L. casei under optimum conditions. 2017-08 Thesis http://eprints.uthm.edu.my/857/ http://eprints.uthm.edu.my/857/2/24p%20SABER%20SALEM%20HASSAN.pdf text en public http://eprints.uthm.edu.my/857/1/SABER%20SALEM%20HASSAN%20COPYRIGHT%20DECLARATION.pdf text en staffonly http://eprints.uthm.edu.my/857/3/SABER%20SALEM%20HASSAN%20WATERMARK.pdf text en validuser mphil masters Universiti Tun Hussein Onn Malaysia Fakulti Sains Gunaan dan Teknologi
institution	Universiti Tun Hussein Onn Malaysia
collection	UTHM Institutional Repository
language	English English English
topic	TD896-899 Industrial and factory wastes
spellingShingle	TD896-899 Industrial and factory wastes Hassan, Saber Salem Production of biodegradable poly lactic acid from pineapple industry wastewater by lactobacillus casei fermentation
description	Pineapple processing industry generates large amount of liquid waste, which contains high concentration of biodegradable organic, suspended solid and high biological oxygen demand (BOD), as well as carbohydrates such as glucose and fructose. The by-products effluent can be a substrate for production of value-added products by microbial fermentation such as biomaterial. However, investigation on the bioconversion of pineapple wastewater to value-added products is still lacking. The objective of this study is to produce biodegradable poly lactic acid (PLA) from pineapple industry waste water by Lactobacillus casei. The L. caseiwas cultivated with a medium containing extracted glucose for 24 h. The optimisation of cultural conditions (temperature, pH, inoculum size, nitrogen source) for lactic acid (LA) production was carried out. Purification of LA by reverse osmosis was done. After that, synthesize of PLA was achieved by direct polymerization of pure LA under a nitrogen atmosphere at an optimum time and temperature. Finally, the characterization of PLA was conducted by gel permeation chromatography (GPC), Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). A maximum production of 31.6 g/l LA was obtained at pH 6, 37 °C and 5% (v/v) of inoculum size. Interestingly, the concentration of pure LA was measured up to 54 g/l after purification. The optimum percentage yield of PLA obtained was 10.4% for 160 °C at 12 h and the molecular weight MW was in the range of 1000 Da to 5215 Da. The highly intense band of 2995 was due to CH stretching of the methylene and methyne groups, a range 1716 to 1748 for ester carbonyl group; other significant bands are in region 1118-1360 typical for ester group -CO-O. A clear glass transition (Tg)at 45.3 °C was exhibited when heat synthesis at 160°C. The results obtained confirmed the production of PLA. Hence it can be concluded that pineapple wastewater can be used for PLA production by L. casei under optimum conditions.
format	Thesis
qualification_name	Master of Philosophy (M.Phil.)
qualification_level	Master's degree
author	Hassan, Saber Salem
author_facet	Hassan, Saber Salem
author_sort	Hassan, Saber Salem
title	Production of biodegradable poly lactic acid from pineapple industry wastewater by lactobacillus casei fermentation
title_short	Production of biodegradable poly lactic acid from pineapple industry wastewater by lactobacillus casei fermentation
title_full	Production of biodegradable poly lactic acid from pineapple industry wastewater by lactobacillus casei fermentation
title_fullStr	Production of biodegradable poly lactic acid from pineapple industry wastewater by lactobacillus casei fermentation
title_full_unstemmed	Production of biodegradable poly lactic acid from pineapple industry wastewater by lactobacillus casei fermentation
title_sort	production of biodegradable poly lactic acid from pineapple industry wastewater by lactobacillus casei fermentation
granting_institution	Universiti Tun Hussein Onn Malaysia
granting_department	Fakulti Sains Gunaan dan Teknologi
publishDate	2017
url	http://eprints.uthm.edu.my/857/2/24p%20SABER%20SALEM%20HASSAN.pdf http://eprints.uthm.edu.my/857/1/SABER%20SALEM%20HASSAN%20COPYRIGHT%20DECLARATION.pdf http://eprints.uthm.edu.my/857/3/SABER%20SALEM%20HASSAN%20WATERMARK.pdf
_version_	1747830695175127040

Production of biodegradable poly lactic acid from pineapple industry wastewater by lactobacillus casei fermentation

Similar Items