Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles

In the present study, the green synthesis and optimization of gold nanoparticles (GNPs) using C. asiatica phenolic extract (CAP) were reported followed by the characterization with UV-vis, FTIR, Zeta potential, FESEM, EDX and XRD analyses. Total phenolic and flavonoid contents (TPC and TFC), antioxi...

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Main Author:	Latif, Muhammad Sohail
Format:	Thesis
Language:	English English
Published:	2019
Subjects:	TX341-641 Nutrition Foods and food supply
Online Access:	http://eprints.uthm.edu.my/91/1/24p%20MUHAMMAD%20SOHAIL%20LATIF.pdf http://eprints.uthm.edu.my/91/2/MUHAMMAD%20SOHAIL%20LATIF%20WATERMARK.pdf
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spelling	my-uthm-ep.912021-06-22T07:52:26Z Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles 2019-07 Latif, Muhammad Sohail TX341-641 Nutrition. Foods and food supply In the present study, the green synthesis and optimization of gold nanoparticles (GNPs) using C. asiatica phenolic extract (CAP) were reported followed by the characterization with UV-vis, FTIR, Zeta potential, FESEM, EDX and XRD analyses. Total phenolic and flavonoid contents (TPC and TFC), antioxidant and anticholinesterase (anti-ChE) activities were also tested in vitro and Pearson correlation analysis was performed. The GNP synthesis using CAP was successfully accomplished under optimum conditions of reactants ratio of 1:1, 8 % CAP concentration, 0.5 mM HAuCl4 concentration and pH 9 at different temperatures of reaction medium (25 ℃, 40 ℃, 55 ℃ and 70 ℃). The results showed that the size range of GNPs was reduced from 6-40 nm at 25 ℃ to 6-30 nm at 40 ℃ and 55 ℃ while the sphericity of GNPs increased and the surface plasmon resonance (SPR) band became narrow. Although all GNP samples showed good antioxidant and anti-ChE activities (% I), the highest activity was recorded for the GNPs synthesized at 55 ℃, that is, 69.06 % and 76.72 % for ABTS and DPPH, and 71.71 % and 69.88 % for AChE and BChE, respectively, at the highest concentration of 500 μL sample. However, the maximum % I for ABTS and DPPH scavenging (79.76±1.01 and 75.51±1.54, respectively) was shown by ascorbic acid, and for AChE and BChE inhibition (75.18±2.29 and 79.94±1.11, respectively) by galanthamine. Pearson correlation analyses revealed a strong positive correlation between antioxidant activity and phenolic content as well as between anti-ChE and antioxidant activities for all GNP samples. The GNPs did not possess a significant cytotoxicity as shown by 20% mortality in brine shrimp lethality assay. The GNPs were haemocompatible as they induced 0.92 % haemolysis at the highest concentration (500 μL) in haemolysis assay compared to 99 % haemolysis by positive control. In conclusion, the results of this study show that CAP-GNPs possess good antioxidant and anticholinesterase activities, in addition to being non-cytotoxic and non-haemolytic, may be an option to further explore their therapeutic potential as antioxidant and cholinesterase inhibitors. 2019-07 Thesis http://eprints.uthm.edu.my/91/ http://eprints.uthm.edu.my/91/1/24p%20MUHAMMAD%20SOHAIL%20LATIF.pdf text en public http://eprints.uthm.edu.my/91/2/MUHAMMAD%20SOHAIL%20LATIF%20WATERMARK.pdf text en validuser phd doctoral Universiti Tun Hussein Onn Malaysia Fakulti Sains Gunaan dan Teknolgi
institution	Universiti Tun Hussein Onn Malaysia
collection	UTHM Institutional Repository
language	English English
topic	TX341-641 Nutrition Foods and food supply
spellingShingle	TX341-641 Nutrition Foods and food supply Latif, Muhammad Sohail Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles
description	In the present study, the green synthesis and optimization of gold nanoparticles (GNPs) using C. asiatica phenolic extract (CAP) were reported followed by the characterization with UV-vis, FTIR, Zeta potential, FESEM, EDX and XRD analyses. Total phenolic and flavonoid contents (TPC and TFC), antioxidant and anticholinesterase (anti-ChE) activities were also tested in vitro and Pearson correlation analysis was performed. The GNP synthesis using CAP was successfully accomplished under optimum conditions of reactants ratio of 1:1, 8 % CAP concentration, 0.5 mM HAuCl4 concentration and pH 9 at different temperatures of reaction medium (25 ℃, 40 ℃, 55 ℃ and 70 ℃). The results showed that the size range of GNPs was reduced from 6-40 nm at 25 ℃ to 6-30 nm at 40 ℃ and 55 ℃ while the sphericity of GNPs increased and the surface plasmon resonance (SPR) band became narrow. Although all GNP samples showed good antioxidant and anti-ChE activities (% I), the highest activity was recorded for the GNPs synthesized at 55 ℃, that is, 69.06 % and 76.72 % for ABTS and DPPH, and 71.71 % and 69.88 % for AChE and BChE, respectively, at the highest concentration of 500 μL sample. However, the maximum % I for ABTS and DPPH scavenging (79.76±1.01 and 75.51±1.54, respectively) was shown by ascorbic acid, and for AChE and BChE inhibition (75.18±2.29 and 79.94±1.11, respectively) by galanthamine. Pearson correlation analyses revealed a strong positive correlation between antioxidant activity and phenolic content as well as between anti-ChE and antioxidant activities for all GNP samples. The GNPs did not possess a significant cytotoxicity as shown by 20% mortality in brine shrimp lethality assay. The GNPs were haemocompatible as they induced 0.92 % haemolysis at the highest concentration (500 μL) in haemolysis assay compared to 99 % haemolysis by positive control. In conclusion, the results of this study show that CAP-GNPs possess good antioxidant and anticholinesterase activities, in addition to being non-cytotoxic and non-haemolytic, may be an option to further explore their therapeutic potential as antioxidant and cholinesterase inhibitors.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Latif, Muhammad Sohail
author_facet	Latif, Muhammad Sohail
author_sort	Latif, Muhammad Sohail
title	Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles
title_short	Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles
title_full	Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles
title_fullStr	Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles
title_full_unstemmed	Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles
title_sort	antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles
granting_institution	Universiti Tun Hussein Onn Malaysia
granting_department	Fakulti Sains Gunaan dan Teknolgi
publishDate	2019
url	http://eprints.uthm.edu.my/91/1/24p%20MUHAMMAD%20SOHAIL%20LATIF.pdf http://eprints.uthm.edu.my/91/2/MUHAMMAD%20SOHAIL%20LATIF%20WATERMARK.pdf
_version_	1747830527776260096

Antioxidant and anticholinesterase activities of centella asiatica phenolic extract-mediated gold nanoparticles

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