Phytobial remediation of arsenic-contaminated soil using Melastoma malabathricum L. and Microbacterium foliorum strain SZ1

Arsenic (As) is an increasing threat across the globe, widely known as a threshold carcinogen, and it is reaching harmful values in several areas of the world. In this present study, the effect of Microbacterium foliorum strain SZ1, a plant growth promoting bacteria (PGPB) on inorganic As (arsenate)...

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Main Author:	Alka, Sadiya
Format:	Thesis
Language:	English
Published:	2022
Subjects:	Q Science (General)
Online Access:	http://eprints.utm.my/id/eprint/101488/1/SadiyaAlkaPFS2022.pdf
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spelling	my-utm-ep.1014882023-06-21T10:17:00Z Phytobial remediation of arsenic-contaminated soil using Melastoma malabathricum L. and Microbacterium foliorum strain SZ1 2022 Alka, Sadiya Q Science (General) Arsenic (As) is an increasing threat across the globe, widely known as a threshold carcinogen, and it is reaching harmful values in several areas of the world. In this present study, the effect of Microbacterium foliorum strain SZ1, a plant growth promoting bacteria (PGPB) on inorganic As (arsenate) phytoremediation by Melastoma malabathricum L. plants were investigated through morphological, histological analysis and proteomic profiling. The experiments included a control treatment (M. malabathricum L. plants without As and bacterial inoculation) and two phytoremediation treatments, M. malabathricum L. in As contaminated soil inoculated or uninoculated with M. foliorum strain SZ1. The morphological parameter and phytoremediation potential induced by M. foliorum strain SZ1 on M. malabathricum L. were observed for 90 days. Besides, the histological and proteome analysis was conducted using Transmission Electron Microscopy (TEM) and 2-Dimensional gel electrophoresis (2-DE), respectively. The present study showed that plants did not display any noticeable signs of toxicity, but rather the root and shoot length were significantly increased in the presence of M. foliorum strain SZ1. In addition, while the Bioconcentration factor (BCF) was increased in the presence of M. foliorum strain SZ1 by 0.3 times, the Transfer factor (TF) of As in the M. malabathricum L. plants were decreased, whether in the presence or absence of PGPB. The As phytoremediation treatment with M. foliorum strain SZ1 also enhanced uptake of As in root (26%) and shoot (22%) than in the other two phytoremediation treatments (As only and control). The findings showed that M. foliorum strain SZ1 inoculation reduced As toxicity by substantially reducing the negative stress effects and increasing shoot and root fresh and dry weight. The repair of As induced structural deformities involving disintegration of cell wall and membranes were observed upon M. foliorum strain SZ1 inoculation. After PD-Quest analysis, 9 differentially expressed spots subjected to liquid chromatography-tandem mass spectrometry and 9 spots identified with a significant score. The up regulation of tubulin, nucleoside diphosphate kinase and major allergen during As with bacteria exposure confirmed the amelioration of As induced oxidative stress. The abundance of proteins involved in photosynthesis, energy metabolism, defence, signaling, protein biogenesis and nucleoside M. malabathricum L. were found higher in As with bacteria than in As alone exposure. Overall, the present study demonstrates the effectiveness of phytoremediation by M. foliorum strain SZ1 on responsive pathways including morphology, histology and proteomic of M. malabathricum L. plant. 2022 Thesis http://eprints.utm.my/id/eprint/101488/ http://eprints.utm.my/id/eprint/101488/1/SadiyaAlkaPFS2022.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:148990 phd doctoral Universiti Teknologi Malaysia, Faculty of Science Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Alka, Sadiya Phytobial remediation of arsenic-contaminated soil using Melastoma malabathricum L. and Microbacterium foliorum strain SZ1
description	Arsenic (As) is an increasing threat across the globe, widely known as a threshold carcinogen, and it is reaching harmful values in several areas of the world. In this present study, the effect of Microbacterium foliorum strain SZ1, a plant growth promoting bacteria (PGPB) on inorganic As (arsenate) phytoremediation by Melastoma malabathricum L. plants were investigated through morphological, histological analysis and proteomic profiling. The experiments included a control treatment (M. malabathricum L. plants without As and bacterial inoculation) and two phytoremediation treatments, M. malabathricum L. in As contaminated soil inoculated or uninoculated with M. foliorum strain SZ1. The morphological parameter and phytoremediation potential induced by M. foliorum strain SZ1 on M. malabathricum L. were observed for 90 days. Besides, the histological and proteome analysis was conducted using Transmission Electron Microscopy (TEM) and 2-Dimensional gel electrophoresis (2-DE), respectively. The present study showed that plants did not display any noticeable signs of toxicity, but rather the root and shoot length were significantly increased in the presence of M. foliorum strain SZ1. In addition, while the Bioconcentration factor (BCF) was increased in the presence of M. foliorum strain SZ1 by 0.3 times, the Transfer factor (TF) of As in the M. malabathricum L. plants were decreased, whether in the presence or absence of PGPB. The As phytoremediation treatment with M. foliorum strain SZ1 also enhanced uptake of As in root (26%) and shoot (22%) than in the other two phytoremediation treatments (As only and control). The findings showed that M. foliorum strain SZ1 inoculation reduced As toxicity by substantially reducing the negative stress effects and increasing shoot and root fresh and dry weight. The repair of As induced structural deformities involving disintegration of cell wall and membranes were observed upon M. foliorum strain SZ1 inoculation. After PD-Quest analysis, 9 differentially expressed spots subjected to liquid chromatography-tandem mass spectrometry and 9 spots identified with a significant score. The up regulation of tubulin, nucleoside diphosphate kinase and major allergen during As with bacteria exposure confirmed the amelioration of As induced oxidative stress. The abundance of proteins involved in photosynthesis, energy metabolism, defence, signaling, protein biogenesis and nucleoside M. malabathricum L. were found higher in As with bacteria than in As alone exposure. Overall, the present study demonstrates the effectiveness of phytoremediation by M. foliorum strain SZ1 on responsive pathways including morphology, histology and proteomic of M. malabathricum L. plant.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Alka, Sadiya
author_facet	Alka, Sadiya
author_sort	Alka, Sadiya
title	Phytobial remediation of arsenic-contaminated soil using Melastoma malabathricum L. and Microbacterium foliorum strain SZ1
title_short	Phytobial remediation of arsenic-contaminated soil using Melastoma malabathricum L. and Microbacterium foliorum strain SZ1
title_full	Phytobial remediation of arsenic-contaminated soil using Melastoma malabathricum L. and Microbacterium foliorum strain SZ1
title_fullStr	Phytobial remediation of arsenic-contaminated soil using Melastoma malabathricum L. and Microbacterium foliorum strain SZ1
title_full_unstemmed	Phytobial remediation of arsenic-contaminated soil using Melastoma malabathricum L. and Microbacterium foliorum strain SZ1
title_sort	phytobial remediation of arsenic-contaminated soil using melastoma malabathricum l. and microbacterium foliorum strain sz1
granting_institution	Universiti Teknologi Malaysia, Faculty of Science
granting_department	Faculty of Science
publishDate	2022
url	http://eprints.utm.my/id/eprint/101488/1/SadiyaAlkaPFS2022.pdf
_version_	1776100709885804544

Phytobial remediation of arsenic-contaminated soil using Melastoma malabathricum L. and Microbacterium foliorum strain SZ1

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