Medium optimization for high cell mass and exopolysaccharides production using paenibacillus polymyxa

Paenibacillus polymyxa ATCC 842 is one of the beneficial microbes that shows high potential to promote plant growth and enhance soil health by producing a variety of beneficial secondary metabolites specifically exopolysaccharides (EPS). This study aimed to optimize medium composition for enhancing...

Full description

Saved in:

Bibliographic Details
Main Author:	Daud, Nur Sazwani
Format:	Thesis
Language:	English
Published:	2022
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/101621/1/NurSazwaniDaudMSChe2022.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-utm-ep.101621
record_format	uketd_dc
spelling	my-utm-ep.1016212023-07-03T02:55:15Z Medium optimization for high cell mass and exopolysaccharides production using paenibacillus polymyxa 2022 Daud, Nur Sazwani TP Chemical technology Paenibacillus polymyxa ATCC 842 is one of the beneficial microbes that shows high potential to promote plant growth and enhance soil health by producing a variety of beneficial secondary metabolites specifically exopolysaccharides (EPS). This study aimed to optimize medium composition for enhancing the production of cell mass and EPS by P. polymyxa ATCC 842 using statistical methods for agriculture application. At first, the one-factor-at-a-time (OFAT) method was used to screen the cultivation medium of P. polymyxa ATCC 842 which was reported previously for high cell mass and EPS production. Then, different ranges concentration of the main components of selected media were investigated such as sucrose, yeast, phosphate and MgSO4. Based on the OFAT results, three factors were selected as the most influence factors: sucrose, yeast extract, and K2HPO4 for the medium optimization through statistical approaches by using response surface methodology (RSM). The Box Behnken design (BBD) was used to evaluate the effects of variables on the cell mass and EPS production. The optimal medium composition by RSM composed of sucrose, 30 g L-1; yeast extract, 30 g L-1; K2HPO4, 5.72 g L-1; NH2NO4, 5 g L-1; KH2PO4, 1.9 g L-1 and MgSO4, 0.5 g L-1 . Under the optimized medium, the cell mass of P. polymyxaATCC 842 about 20.36 g L-1, which was 7.51-fold higher than the unoptimized medium (2.71 gL-1). Meanwhile, the EPS was increased 16.46 g L-1, about 6.71-foldhigher than the unoptimized medium (2.45 g L-1) after 36 hours of cultivation at pH7.0, 30 °C, and agitation speed at 150 rpm. The model was found to be significant and subsequently validated through the growth kinetics studies in the unoptimized andoptimized medium by RSM. The result revealed that the optimized medium was ableto support better growth of P. polymyxa ATCC 842 with a specific growth rate of 1.397h-1 and EPS productivity about 0.68 g L-1h-1. Furthermore, to understand the physio-chemical of EPS from P. polymyxa ATCC 842, a series of biochemistry characteristics were investigated. High-Performance Liquid Chromatography (HPLC) analysis revealed that EPS of P. polymyxa ATCC 842 is composed of monomer fructose and glucose. Meanwhile, Fourier-transforms infrared spectroscopy analysis showed the presence of hydroxyl, carboxyl groups, and glycosidic linkages which showed the composition of the polysaccharide bound by a-(1?6). The scanning electron micrographs analysis showed EPS appears as a homogenous spherical with irregular porosity for high water holding capacity of the soil. The EPS structure could result ina higher water absorption/ retention capacity, which is an attractive feature for a sticky agent for soil aggregation. P. polymyxa ATCC 842 with the spent mushroom substrate(SMS) as soil amendment gives the best result in neutralizing the pH and forming soil aggregate including phosphate and potassium improvements. In conclusion, the optimized production medium shows significant effects on enhancing the growth of P.polymyxa ATCC 842 and maximum secretion of EPS and indirectly, promotes the application of P. polymxya ATCC 842 as the beneficial candidate for sustainable agricultural practices. 2022 Thesis http://eprints.utm.my/id/eprint/101621/ http://eprints.utm.my/id/eprint/101621/1/NurSazwaniDaudMSChe2022.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:150719 masters Universiti Teknologi Malaysia Faculty of Engineering - School of Chemical & Energy Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Daud, Nur Sazwani Medium optimization for high cell mass and exopolysaccharides production using paenibacillus polymyxa
description	Paenibacillus polymyxa ATCC 842 is one of the beneficial microbes that shows high potential to promote plant growth and enhance soil health by producing a variety of beneficial secondary metabolites specifically exopolysaccharides (EPS). This study aimed to optimize medium composition for enhancing the production of cell mass and EPS by P. polymyxa ATCC 842 using statistical methods for agriculture application. At first, the one-factor-at-a-time (OFAT) method was used to screen the cultivation medium of P. polymyxa ATCC 842 which was reported previously for high cell mass and EPS production. Then, different ranges concentration of the main components of selected media were investigated such as sucrose, yeast, phosphate and MgSO4. Based on the OFAT results, three factors were selected as the most influence factors: sucrose, yeast extract, and K2HPO4 for the medium optimization through statistical approaches by using response surface methodology (RSM). The Box Behnken design (BBD) was used to evaluate the effects of variables on the cell mass and EPS production. The optimal medium composition by RSM composed of sucrose, 30 g L-1; yeast extract, 30 g L-1; K2HPO4, 5.72 g L-1; NH2NO4, 5 g L-1; KH2PO4, 1.9 g L-1 and MgSO4, 0.5 g L-1 . Under the optimized medium, the cell mass of P. polymyxaATCC 842 about 20.36 g L-1, which was 7.51-fold higher than the unoptimized medium (2.71 gL-1). Meanwhile, the EPS was increased 16.46 g L-1, about 6.71-foldhigher than the unoptimized medium (2.45 g L-1) after 36 hours of cultivation at pH7.0, 30 °C, and agitation speed at 150 rpm. The model was found to be significant and subsequently validated through the growth kinetics studies in the unoptimized andoptimized medium by RSM. The result revealed that the optimized medium was ableto support better growth of P. polymyxa ATCC 842 with a specific growth rate of 1.397h-1 and EPS productivity about 0.68 g L-1h-1. Furthermore, to understand the physio-chemical of EPS from P. polymyxa ATCC 842, a series of biochemistry characteristics were investigated. High-Performance Liquid Chromatography (HPLC) analysis revealed that EPS of P. polymyxa ATCC 842 is composed of monomer fructose and glucose. Meanwhile, Fourier-transforms infrared spectroscopy analysis showed the presence of hydroxyl, carboxyl groups, and glycosidic linkages which showed the composition of the polysaccharide bound by a-(1?6). The scanning electron micrographs analysis showed EPS appears as a homogenous spherical with irregular porosity for high water holding capacity of the soil. The EPS structure could result ina higher water absorption/ retention capacity, which is an attractive feature for a sticky agent for soil aggregation. P. polymyxa ATCC 842 with the spent mushroom substrate(SMS) as soil amendment gives the best result in neutralizing the pH and forming soil aggregate including phosphate and potassium improvements. In conclusion, the optimized production medium shows significant effects on enhancing the growth of P.polymyxa ATCC 842 and maximum secretion of EPS and indirectly, promotes the application of P. polymxya ATCC 842 as the beneficial candidate for sustainable agricultural practices.
format	Thesis
qualification_level	Master's degree
author	Daud, Nur Sazwani
author_facet	Daud, Nur Sazwani
author_sort	Daud, Nur Sazwani
title	Medium optimization for high cell mass and exopolysaccharides production using paenibacillus polymyxa
title_short	Medium optimization for high cell mass and exopolysaccharides production using paenibacillus polymyxa
title_full	Medium optimization for high cell mass and exopolysaccharides production using paenibacillus polymyxa
title_fullStr	Medium optimization for high cell mass and exopolysaccharides production using paenibacillus polymyxa
title_full_unstemmed	Medium optimization for high cell mass and exopolysaccharides production using paenibacillus polymyxa
title_sort	medium optimization for high cell mass and exopolysaccharides production using paenibacillus polymyxa
granting_institution	Universiti Teknologi Malaysia
granting_department	Faculty of Engineering - School of Chemical & Energy Engineering
publishDate	2022
url	http://eprints.utm.my/id/eprint/101621/1/NurSazwaniDaudMSChe2022.pdf
_version_	1776100740275634176

Medium optimization for high cell mass and exopolysaccharides production using paenibacillus polymyxa

Similar Items