Antibacterial activity of biosynthesized silver nanoparticles using clinacanthus nutans extract

Several techniques were used to produce nanoparticles due to various advantages and disadvantages. Hence, the suggested method to overcome this problem was a ‘green’ method. This study employed Clinacanthus nutans (C. nutans) extract in the synthesis of silver nanoparticles (Ag NPs) and evaluated it...

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Main Author:	Waiezi, Salahaedin
Format:	Thesis
Language:	English
Published:	2020
Subjects:	QD Chemistry
Online Access:	http://eprints.utm.my/id/eprint/101757/1/SalahaedinWaieziMFS2020.pdf
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spelling	my-utm-ep.1017572023-07-10T09:23:43Z Antibacterial activity of biosynthesized silver nanoparticles using clinacanthus nutans extract 2020 Waiezi, Salahaedin QD Chemistry Several techniques were used to produce nanoparticles due to various advantages and disadvantages. Hence, the suggested method to overcome this problem was a ‘green’ method. This study employed Clinacanthus nutans (C. nutans) extract in the synthesis of silver nanoparticles (Ag NPs) and evaluated its antibacterial potential. In this study, Ultraviolet-visible spectroscopy (UV-Vis) result revealed that the formation of the Ag NPs with an absorption peak at approximately 380 to 420 nm is an optimized condition. The synthesized Ag NPs was then characterized based on the particle size, shape and morphology using X-Ray Powder Diffraction Analysis (XRD), Energy Dispersive X-ray spectroscopy (EDX), Transmission Electron Microscopy (TEM), and Field Emission Scanning Electron Microscopy (FESEM). The XRD confirmed the crystal nature of the Ag NPs while the FESEM showed the spherical shape of the Ag NPs. FESEM and TEM indicated the range of Ag NPs size is in between 20 – 30 nm with a spherical shape. Subsequently, the antibacterial activity of the synthesized Ag NPs through Disk Diffusion Technique (DDT) indicated effective inhibition against a few bacteria including Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. In contrast, there was a nominal antibacterial activity against Enterococcus faecalis and Methicillin-resistant Staphylococcus aureus. In general, this study focuses on the potential of C. nutans in the reduction, stabilization, capping, and synthesizing the Ag NPs as an environment economic, and easy technique. 2020 Thesis http://eprints.utm.my/id/eprint/101757/ http://eprints.utm.my/id/eprint/101757/1/SalahaedinWaieziMFS2020.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:145875 masters Universiti Teknologi Malaysia Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Waiezi, Salahaedin Antibacterial activity of biosynthesized silver nanoparticles using clinacanthus nutans extract
description	Several techniques were used to produce nanoparticles due to various advantages and disadvantages. Hence, the suggested method to overcome this problem was a ‘green’ method. This study employed Clinacanthus nutans (C. nutans) extract in the synthesis of silver nanoparticles (Ag NPs) and evaluated its antibacterial potential. In this study, Ultraviolet-visible spectroscopy (UV-Vis) result revealed that the formation of the Ag NPs with an absorption peak at approximately 380 to 420 nm is an optimized condition. The synthesized Ag NPs was then characterized based on the particle size, shape and morphology using X-Ray Powder Diffraction Analysis (XRD), Energy Dispersive X-ray spectroscopy (EDX), Transmission Electron Microscopy (TEM), and Field Emission Scanning Electron Microscopy (FESEM). The XRD confirmed the crystal nature of the Ag NPs while the FESEM showed the spherical shape of the Ag NPs. FESEM and TEM indicated the range of Ag NPs size is in between 20 – 30 nm with a spherical shape. Subsequently, the antibacterial activity of the synthesized Ag NPs through Disk Diffusion Technique (DDT) indicated effective inhibition against a few bacteria including Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. In contrast, there was a nominal antibacterial activity against Enterococcus faecalis and Methicillin-resistant Staphylococcus aureus. In general, this study focuses on the potential of C. nutans in the reduction, stabilization, capping, and synthesizing the Ag NPs as an environment economic, and easy technique.
format	Thesis
qualification_level	Master's degree
author	Waiezi, Salahaedin
author_facet	Waiezi, Salahaedin
author_sort	Waiezi, Salahaedin
title	Antibacterial activity of biosynthesized silver nanoparticles using clinacanthus nutans extract
title_short	Antibacterial activity of biosynthesized silver nanoparticles using clinacanthus nutans extract
title_full	Antibacterial activity of biosynthesized silver nanoparticles using clinacanthus nutans extract
title_fullStr	Antibacterial activity of biosynthesized silver nanoparticles using clinacanthus nutans extract
title_full_unstemmed	Antibacterial activity of biosynthesized silver nanoparticles using clinacanthus nutans extract
title_sort	antibacterial activity of biosynthesized silver nanoparticles using clinacanthus nutans extract
granting_institution	Universiti Teknologi Malaysia
granting_department	Faculty of Science
publishDate	2020
url	http://eprints.utm.my/id/eprint/101757/1/SalahaedinWaieziMFS2020.pdf
_version_	1776100764310044672

Antibacterial activity of biosynthesized silver nanoparticles using clinacanthus nutans extract

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