Bioprocess optimization for high cell mass production of Lactobacillus acidophilus as probiotic

Lactobacillus from the lactic acid bacteria group, mainly synonym as a good bacterium known as probiotics that give a beneficial effect on human health. Lactobacillus acidophilus is one of the important bacteria that can maintain and restore gastrointestinal microflora, rebuild the digestive system...

Full description

Saved in:

Bibliographic Details
Main Author:	Kepli, Afif Najihah
Format:	Thesis
Language:	English
Published:	2021
Subjects:	TP Chemical technology
Online Access:	http://eprints.utm.my/id/eprint/101885/1/AfifNajihahKepliMFChE2021.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-utm-ep.101885
record_format	uketd_dc
spelling	my-utm-ep.1018852023-07-22T03:16:12Z Bioprocess optimization for high cell mass production of Lactobacillus acidophilus as probiotic 2021 Kepli, Afif Najihah TP Chemical technology Lactobacillus from the lactic acid bacteria group, mainly synonym as a good bacterium known as probiotics that give a beneficial effect on human health. Lactobacillus acidophilus is one of the important bacteria that can maintain and restore gastrointestinal microflora, rebuild the digestive system from harmful bacteria, and fight vaginal infection. However, the biomass production of this bacteria is one of the industrial challenges. Therefore, this study was carried out to maximize cell mass production through optimization of medium composition and scaling up of the process to semi-industrial scale. Twelve different media were screened for the potential effect on cell growth. The best medium was composed of (g L-1): glucose, 30; yeast extract, 6; ammonium citrate, 1; citric acid, 0.5; potassium dihydrogen phosphate (KH2PO4), 1.5; magnesium sulphate heptahydrate (MgSO4.7H2O), 0.4; manganese (II) sulphate monohydrate (MnSO4.H2O), 0.082; sodium acetate, 1; and tween 80, 1. The biomass produced in this medium reached 2.46 g L-1. Further medium optimization using one factor at a time (OFAT) and statistical method response surface methodology (RSM) improved biomass production up to 4.64 g L-1 and 5.36 g L-1, respectively. The RSM optimized medium supported biomass production by approximately 15.52 % compared to OFAT optimized medium. Thus, the RSM optimized medium was used further in a 16-L bioreactor operated in batch cultivation mode to increase cell mass production. Cultivations in the bioreactor were carried out under controlled and uncontrolled pH conditions. High cell mass production was achieved in a controlled pH bioreactor (pH 6.5) and reached 6.41 g L-1 compared to in an un-controlled pH bioreactor which produced 4.56 g L-1 only. The biomass obtained from the controlled pH bioreactor was used for microencapsulation process. The cell viability after encapsulation was 9.45 log CFU/g with 76.95 % of encapsulation efficiency. The encapsulated L. acidophilus exhibited good resistance to bile salt concentration with 77 % of cells survived at bile salt concentration of 0.3%. However, resistance to the bile salt was found to be affected by pH value as well. After two hours of treatment, cell viability was 31.84 % at pH 4, whereas, cells were completely inactivated at pH 1. Thus, it can be concluded that statistically optimized medium composed of (g L-1): glucose, 50; yeast extract, 20.91; ammonium citrate, 3.42; citric acid, 0.5; KH2PO4, 1.5; MgSO4.7H2O, 0.4; MnSO4.H2O, 0.082; sodium acetate, 1; and tween 80, 1 produced the highest biomass production under pH-controlled condition of 6.5. Microencapsulation was also a suitable approach to protect cell viability when further applied to the human gastrointestinal tract. 2021 Thesis http://eprints.utm.my/id/eprint/101885/ http://eprints.utm.my/id/eprint/101885/1/AfifNajihahKepliMFChE2021.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:145602 masters Universiti Teknologi Malaysia Faculty of Engineering - School of Chemical & Energy Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Kepli, Afif Najihah Bioprocess optimization for high cell mass production of Lactobacillus acidophilus as probiotic
description	Lactobacillus from the lactic acid bacteria group, mainly synonym as a good bacterium known as probiotics that give a beneficial effect on human health. Lactobacillus acidophilus is one of the important bacteria that can maintain and restore gastrointestinal microflora, rebuild the digestive system from harmful bacteria, and fight vaginal infection. However, the biomass production of this bacteria is one of the industrial challenges. Therefore, this study was carried out to maximize cell mass production through optimization of medium composition and scaling up of the process to semi-industrial scale. Twelve different media were screened for the potential effect on cell growth. The best medium was composed of (g L-1): glucose, 30; yeast extract, 6; ammonium citrate, 1; citric acid, 0.5; potassium dihydrogen phosphate (KH2PO4), 1.5; magnesium sulphate heptahydrate (MgSO4.7H2O), 0.4; manganese (II) sulphate monohydrate (MnSO4.H2O), 0.082; sodium acetate, 1; and tween 80, 1. The biomass produced in this medium reached 2.46 g L-1. Further medium optimization using one factor at a time (OFAT) and statistical method response surface methodology (RSM) improved biomass production up to 4.64 g L-1 and 5.36 g L-1, respectively. The RSM optimized medium supported biomass production by approximately 15.52 % compared to OFAT optimized medium. Thus, the RSM optimized medium was used further in a 16-L bioreactor operated in batch cultivation mode to increase cell mass production. Cultivations in the bioreactor were carried out under controlled and uncontrolled pH conditions. High cell mass production was achieved in a controlled pH bioreactor (pH 6.5) and reached 6.41 g L-1 compared to in an un-controlled pH bioreactor which produced 4.56 g L-1 only. The biomass obtained from the controlled pH bioreactor was used for microencapsulation process. The cell viability after encapsulation was 9.45 log CFU/g with 76.95 % of encapsulation efficiency. The encapsulated L. acidophilus exhibited good resistance to bile salt concentration with 77 % of cells survived at bile salt concentration of 0.3%. However, resistance to the bile salt was found to be affected by pH value as well. After two hours of treatment, cell viability was 31.84 % at pH 4, whereas, cells were completely inactivated at pH 1. Thus, it can be concluded that statistically optimized medium composed of (g L-1): glucose, 50; yeast extract, 20.91; ammonium citrate, 3.42; citric acid, 0.5; KH2PO4, 1.5; MgSO4.7H2O, 0.4; MnSO4.H2O, 0.082; sodium acetate, 1; and tween 80, 1 produced the highest biomass production under pH-controlled condition of 6.5. Microencapsulation was also a suitable approach to protect cell viability when further applied to the human gastrointestinal tract.
format	Thesis
qualification_level	Master's degree
author	Kepli, Afif Najihah
author_facet	Kepli, Afif Najihah
author_sort	Kepli, Afif Najihah
title	Bioprocess optimization for high cell mass production of Lactobacillus acidophilus as probiotic
title_short	Bioprocess optimization for high cell mass production of Lactobacillus acidophilus as probiotic
title_full	Bioprocess optimization for high cell mass production of Lactobacillus acidophilus as probiotic
title_fullStr	Bioprocess optimization for high cell mass production of Lactobacillus acidophilus as probiotic
title_full_unstemmed	Bioprocess optimization for high cell mass production of Lactobacillus acidophilus as probiotic
title_sort	bioprocess optimization for high cell mass production of lactobacillus acidophilus as probiotic
granting_institution	Universiti Teknologi Malaysia
granting_department	Faculty of Engineering - School of Chemical & Energy Engineering
publishDate	2021
url	http://eprints.utm.my/id/eprint/101885/1/AfifNajihahKepliMFChE2021.pdf
_version_	1776100794985086976

Bioprocess optimization for high cell mass production of Lactobacillus acidophilus as probiotic

Similar Items