Characterisation of mannanase from Pontibacillus sp. CL43

Lignocellulosic biomass is a renewable feedstock for biofuel production. Pretreatment is performed to destroy the biomass recalcitrant structure subsequently allow enzymes to hydrolyse cellulose and hemicellulose for sugars production. These sugars could be used for biofuel production. In chemical p...

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Main Author: Tan, Sing Ngoh
Format: Thesis
Language:English
Published: 2020
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Online Access:http://eprints.utm.my/id/eprint/101923/1/TanSingNgohMFS2020.pdf
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spelling my-utm-ep.1019232023-07-25T09:31:16Z Characterisation of mannanase from Pontibacillus sp. CL43 2020 Tan, Sing Ngoh QD Chemistry Lignocellulosic biomass is a renewable feedstock for biofuel production. Pretreatment is performed to destroy the biomass recalcitrant structure subsequently allow enzymes to hydrolyse cellulose and hemicellulose for sugars production. These sugars could be used for biofuel production. In chemical pretreatment of lignocellulosic biomass, metal salts (KCl, CaCl2 and MgCl2) and surfactants (Tween 20, Tween 80 and Triton X-100) are used. These compounds retain in the biomass after the treatment and affect the performance of enzymes in following hydrolysis process. Hence, enzymes with metal- and surfactant-tolerance characteristics are preferable in biofuel production. This project focused on characterising Pontibacillus sp. CL43 and its mannanase which is responsible for degradation of mannan, a component present in lignocellulosic biomass. Strain CL43 is a Gram positive bacterium with rod-shaped, endospore-forming, catalase- and oxidase-positive. Strain CL43 could hydrolyse bile esculin, casein, starch, xylan, Tween 20, Tween 40, Tween 60 and Tween 80. Strain CL43 was susceptible to ampicillin, carbenicillin, doxycycline, gentamicin, minocycline, neomycin, oxacillin, penicillin G, piperacillin, polymyxin B, rifampicin, streptomycin and tetracycline. Mannanase of strain CL43 exhibited optimal activity at 60 °C, pH 7 and 2% (w/v) NaCl. Mannanase activity was found to be stable in the presence of metal ions (Ca2+, Cd2+, Mn2+, K+, Mg2+ and Co2+ with relative activity ranged from 87-128%) and surfactants (Tween 20, Tween 40, Tween 60, Tween 80 and Triton X-100 with relative activity ranged from 88-107%). Mannanase of strain CL43 with good tolerance to these metal ions and surfactants indicated it is a good candidate to be used for lignocellulosic biomass hydrolysis. 2020 Thesis http://eprints.utm.my/id/eprint/101923/ http://eprints.utm.my/id/eprint/101923/1/TanSingNgohMFS2020.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:146356 masters Universiti Teknologi Malaysia Faculty of Science
institution Universiti Teknologi Malaysia
collection UTM Institutional Repository
language English
topic QD Chemistry
spellingShingle QD Chemistry
Tan, Sing Ngoh
Characterisation of mannanase from Pontibacillus sp. CL43
description Lignocellulosic biomass is a renewable feedstock for biofuel production. Pretreatment is performed to destroy the biomass recalcitrant structure subsequently allow enzymes to hydrolyse cellulose and hemicellulose for sugars production. These sugars could be used for biofuel production. In chemical pretreatment of lignocellulosic biomass, metal salts (KCl, CaCl2 and MgCl2) and surfactants (Tween 20, Tween 80 and Triton X-100) are used. These compounds retain in the biomass after the treatment and affect the performance of enzymes in following hydrolysis process. Hence, enzymes with metal- and surfactant-tolerance characteristics are preferable in biofuel production. This project focused on characterising Pontibacillus sp. CL43 and its mannanase which is responsible for degradation of mannan, a component present in lignocellulosic biomass. Strain CL43 is a Gram positive bacterium with rod-shaped, endospore-forming, catalase- and oxidase-positive. Strain CL43 could hydrolyse bile esculin, casein, starch, xylan, Tween 20, Tween 40, Tween 60 and Tween 80. Strain CL43 was susceptible to ampicillin, carbenicillin, doxycycline, gentamicin, minocycline, neomycin, oxacillin, penicillin G, piperacillin, polymyxin B, rifampicin, streptomycin and tetracycline. Mannanase of strain CL43 exhibited optimal activity at 60 °C, pH 7 and 2% (w/v) NaCl. Mannanase activity was found to be stable in the presence of metal ions (Ca2+, Cd2+, Mn2+, K+, Mg2+ and Co2+ with relative activity ranged from 87-128%) and surfactants (Tween 20, Tween 40, Tween 60, Tween 80 and Triton X-100 with relative activity ranged from 88-107%). Mannanase of strain CL43 with good tolerance to these metal ions and surfactants indicated it is a good candidate to be used for lignocellulosic biomass hydrolysis.
format Thesis
qualification_level Master's degree
author Tan, Sing Ngoh
author_facet Tan, Sing Ngoh
author_sort Tan, Sing Ngoh
title Characterisation of mannanase from Pontibacillus sp. CL43
title_short Characterisation of mannanase from Pontibacillus sp. CL43
title_full Characterisation of mannanase from Pontibacillus sp. CL43
title_fullStr Characterisation of mannanase from Pontibacillus sp. CL43
title_full_unstemmed Characterisation of mannanase from Pontibacillus sp. CL43
title_sort characterisation of mannanase from pontibacillus sp. cl43
granting_institution Universiti Teknologi Malaysia
granting_department Faculty of Science
publishDate 2020
url http://eprints.utm.my/id/eprint/101923/1/TanSingNgohMFS2020.pdf
_version_ 1776100804170612736