Solid phase extraction and characterization of rutin from Labisia pumila var. Alata

Rutin is one of the glycosylated flavonoids which is widely investigated by researchers from buckwheat samples due to its biological activities, including antioxidant activity. Interestingly, rutin was also found in L. pumila var. Alata extract, particularly from the leaves of the plants (0.85 mg of...

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Main Author:	Ruzlan, Nur Nabihah
Format:	Thesis
Language:	English
Published:	2015
Subjects:	Q Science (General)
Online Access:	http://eprints.utm.my/id/eprint/101933/1/NurNabihahRuzlanMSChE2015.pdf
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spelling	my-utm-ep.1019332023-07-22T04:06:43Z Solid phase extraction and characterization of rutin from Labisia pumila var. Alata 2015 Ruzlan, Nur Nabihah Q Science (General) Rutin is one of the glycosylated flavonoids which is widely investigated by researchers from buckwheat samples due to its biological activities, including antioxidant activity. Interestingly, rutin was also found in L. pumila var. Alata extract, particularly from the leaves of the plants (0.85 mg of rutin/ g of leaves). This study focused on the optimization of methanolic solvent system for rutin elution from the crude extract of Labisia pumila var. Alata using C18 reversed phase solid phase extraction (SPE). The rutin fraction was identified and characterised by using thin layer chromatography (Rf 0.49) and UV-Vis spectrophotometry (285 nm). The presence of rutin was further confirmed and quantified by using a liquid chromatography integrated with tandem mass spectrometer based on rutin ion transition (m/z 609 > 301) in the negative mode of targeted analysis. Methanolic extraction (60% methanol) was found to increase the rutin content (0.14% w/w) in the crude extract. Fractionation of the crude extract using 80% methanol as the solvent system had further increased the rutin content to 1.31% w/w. The fractionation was carried out in a laboratory scale SPE system using C18 packed column with the minimum volume of solvent usage (20 mL) with high yield of recovery (76.88%). The rutin fraction (IC50 800 ppm) also exhibited 9.5 times lower scavenging activity than ascorbic acid (IC50 84 ppm) and 6.6 times lower scavenging activity than standard rutin (IC50 122 ppm). However, it showed 1.9 times higher scavenging activity than crude extract (IC50 1500 ppm). A three-layer artificial neural network (ANN) model was developed to predict the yield of rutin based on the polarity of solvent system and volume of eluent. This ANN model produced a good prediction of the experimental data with a correlation coefficient of (R2) 0.9998 and 1.0 for training and testing data, respectively. As a conclusion, a laboratory scale SPE system for rutin elution from the crude extract of L. pumila var. Alata has been established in the current study. 2015 Thesis http://eprints.utm.my/id/eprint/101933/ http://eprints.utm.my/id/eprint/101933/1/NurNabihahRuzlanMSChE2015.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:147939 masters Universiti Teknologi Malaysia, Faculty of Engineering - School of Chemical & Energy Engineering Faculty of Engineering - School of Chemical & Energy Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Ruzlan, Nur Nabihah Solid phase extraction and characterization of rutin from Labisia pumila var. Alata
description	Rutin is one of the glycosylated flavonoids which is widely investigated by researchers from buckwheat samples due to its biological activities, including antioxidant activity. Interestingly, rutin was also found in L. pumila var. Alata extract, particularly from the leaves of the plants (0.85 mg of rutin/ g of leaves). This study focused on the optimization of methanolic solvent system for rutin elution from the crude extract of Labisia pumila var. Alata using C18 reversed phase solid phase extraction (SPE). The rutin fraction was identified and characterised by using thin layer chromatography (Rf 0.49) and UV-Vis spectrophotometry (285 nm). The presence of rutin was further confirmed and quantified by using a liquid chromatography integrated with tandem mass spectrometer based on rutin ion transition (m/z 609 > 301) in the negative mode of targeted analysis. Methanolic extraction (60% methanol) was found to increase the rutin content (0.14% w/w) in the crude extract. Fractionation of the crude extract using 80% methanol as the solvent system had further increased the rutin content to 1.31% w/w. The fractionation was carried out in a laboratory scale SPE system using C18 packed column with the minimum volume of solvent usage (20 mL) with high yield of recovery (76.88%). The rutin fraction (IC50 800 ppm) also exhibited 9.5 times lower scavenging activity than ascorbic acid (IC50 84 ppm) and 6.6 times lower scavenging activity than standard rutin (IC50 122 ppm). However, it showed 1.9 times higher scavenging activity than crude extract (IC50 1500 ppm). A three-layer artificial neural network (ANN) model was developed to predict the yield of rutin based on the polarity of solvent system and volume of eluent. This ANN model produced a good prediction of the experimental data with a correlation coefficient of (R2) 0.9998 and 1.0 for training and testing data, respectively. As a conclusion, a laboratory scale SPE system for rutin elution from the crude extract of L. pumila var. Alata has been established in the current study.
format	Thesis
qualification_level	Master's degree
author	Ruzlan, Nur Nabihah
author_facet	Ruzlan, Nur Nabihah
author_sort	Ruzlan, Nur Nabihah
title	Solid phase extraction and characterization of rutin from Labisia pumila var. Alata
title_short	Solid phase extraction and characterization of rutin from Labisia pumila var. Alata
title_full	Solid phase extraction and characterization of rutin from Labisia pumila var. Alata
title_fullStr	Solid phase extraction and characterization of rutin from Labisia pumila var. Alata
title_full_unstemmed	Solid phase extraction and characterization of rutin from Labisia pumila var. Alata
title_sort	solid phase extraction and characterization of rutin from labisia pumila var. alata
granting_institution	Universiti Teknologi Malaysia, Faculty of Engineering - School of Chemical & Energy Engineering
granting_department	Faculty of Engineering - School of Chemical & Energy Engineering
publishDate	2015
url	http://eprints.utm.my/id/eprint/101933/1/NurNabihahRuzlanMSChE2015.pdf
_version_	1776100806581288960

Solid phase extraction and characterization of rutin from Labisia pumila var. Alata

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