Chemical profiling and optimization of rhoifolin extraction in relation to antioxidant properties from Fortunella polyandra

The chemical profile and optimization of extraction process towards antioxidant response of Fortunella polyandra were studied. Essential oils from the leaves, peels, and twigs of F. polyandra were obtained by hydrodistillation and analyzed using gas chromatographymass spectrometry (GC-MS). a-Eudesmo...

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Main Author:	Elias, Hazim Syahmi
Format:	Thesis
Language:	English
Published:	2021
Subjects:	QD Chemistry
Online Access:	http://eprints.utm.my/id/eprint/101943/1/HazimSyahmiEliasMFS2021.pdf
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spelling	my-utm-ep.1019432023-07-25T09:41:26Z Chemical profiling and optimization of rhoifolin extraction in relation to antioxidant properties from Fortunella polyandra 2021 Elias, Hazim Syahmi QD Chemistry The chemical profile and optimization of extraction process towards antioxidant response of Fortunella polyandra were studied. Essential oils from the leaves, peels, and twigs of F. polyandra were obtained by hydrodistillation and analyzed using gas chromatographymass spectrometry (GC-MS). a-Eudesmol (31.0%), hedycrayol (20.1%) and ?-eudesmol (19.7%) were the major components in the leaves oil. The most abundant components of the peels oil consisted of limonene (71.4%) and ?-terpinene (15.2%), while a-gurjunene (26.4%), hedycrayol (23.5%), and ?-eudesmol (22.1%) were the main constituents in the twigs oil. Chromatographic separation on the hexane and methanol extract has yielded lupeol and a mixture of ?-sitosterol, stigmasterol and campesterol, respectively. The chemical composition of the leaves methanolic extract was determined using ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QToF-MS/MS). Six flavonoid glycosides were identified and tentatively characterized as 3',5'-di-C-ß-glucopyranosylphloretin, apigenin 8-Cneohesperidoside, acacetin 6-C-neohesperidoside, acacetin 8-C-neohesperidoside, apigenin 6,8-di-C-glucoside and apigenin 7-O-neohesperidoside (rhoifolin). The influence of the extraction parameters, namely temperatures (30–70°C), times (5–15 minutes), and solvent ratios (30:70–70:30) of ethanol:water were investigated using response surface methodology (RSM) in order to determine the optimum extraction conditions that could produce maximum antioxidant activity from F. polyandra extract. Optimal extraction conditions for 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity were obtained at extraction time of 5 minutes, temperature of 30°C and solvent ratio of 70:30, ethanol:water. The obtained IC50 value in the DPPH assay was 0.126±0.004 mg/mL, which is close to the predicted value of 0.137 mg/mL. The optimum parameters for ferric reducing antioxidant potential (FRAP) assay were extraction time of 5 min, temperature of 30°C, and solvent ratio of 30:70, ethanol:water. Under these conditions, rhoifolin content obtained was 67.83±0.37 ppm, which is very close to the predicted value of 67.58 ppm, whereas FRAP equivalent value of 0.22±0.01 mg/mL obtained is equal to the predicted value from the model. All three responses from the model achieved 95% confidence level. Correlation study conducted shows that there is no relationship between the rhoifolin content and the antioxidant activities of F. polyandra extract. 2021 Thesis http://eprints.utm.my/id/eprint/101943/ http://eprints.utm.my/id/eprint/101943/1/HazimSyahmiEliasMFS2021.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:146294 masters Universiti Teknologi Malaysia Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Elias, Hazim Syahmi Chemical profiling and optimization of rhoifolin extraction in relation to antioxidant properties from Fortunella polyandra
description	The chemical profile and optimization of extraction process towards antioxidant response of Fortunella polyandra were studied. Essential oils from the leaves, peels, and twigs of F. polyandra were obtained by hydrodistillation and analyzed using gas chromatographymass spectrometry (GC-MS). a-Eudesmol (31.0%), hedycrayol (20.1%) and ?-eudesmol (19.7%) were the major components in the leaves oil. The most abundant components of the peels oil consisted of limonene (71.4%) and ?-terpinene (15.2%), while a-gurjunene (26.4%), hedycrayol (23.5%), and ?-eudesmol (22.1%) were the main constituents in the twigs oil. Chromatographic separation on the hexane and methanol extract has yielded lupeol and a mixture of ?-sitosterol, stigmasterol and campesterol, respectively. The chemical composition of the leaves methanolic extract was determined using ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QToF-MS/MS). Six flavonoid glycosides were identified and tentatively characterized as 3',5'-di-C-ß-glucopyranosylphloretin, apigenin 8-Cneohesperidoside, acacetin 6-C-neohesperidoside, acacetin 8-C-neohesperidoside, apigenin 6,8-di-C-glucoside and apigenin 7-O-neohesperidoside (rhoifolin). The influence of the extraction parameters, namely temperatures (30–70°C), times (5–15 minutes), and solvent ratios (30:70–70:30) of ethanol:water were investigated using response surface methodology (RSM) in order to determine the optimum extraction conditions that could produce maximum antioxidant activity from F. polyandra extract. Optimal extraction conditions for 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity were obtained at extraction time of 5 minutes, temperature of 30°C and solvent ratio of 70:30, ethanol:water. The obtained IC50 value in the DPPH assay was 0.126±0.004 mg/mL, which is close to the predicted value of 0.137 mg/mL. The optimum parameters for ferric reducing antioxidant potential (FRAP) assay were extraction time of 5 min, temperature of 30°C, and solvent ratio of 30:70, ethanol:water. Under these conditions, rhoifolin content obtained was 67.83±0.37 ppm, which is very close to the predicted value of 67.58 ppm, whereas FRAP equivalent value of 0.22±0.01 mg/mL obtained is equal to the predicted value from the model. All three responses from the model achieved 95% confidence level. Correlation study conducted shows that there is no relationship between the rhoifolin content and the antioxidant activities of F. polyandra extract.
format	Thesis
qualification_level	Master's degree
author	Elias, Hazim Syahmi
author_facet	Elias, Hazim Syahmi
author_sort	Elias, Hazim Syahmi
title	Chemical profiling and optimization of rhoifolin extraction in relation to antioxidant properties from Fortunella polyandra
title_short	Chemical profiling and optimization of rhoifolin extraction in relation to antioxidant properties from Fortunella polyandra
title_full	Chemical profiling and optimization of rhoifolin extraction in relation to antioxidant properties from Fortunella polyandra
title_fullStr	Chemical profiling and optimization of rhoifolin extraction in relation to antioxidant properties from Fortunella polyandra
title_full_unstemmed	Chemical profiling and optimization of rhoifolin extraction in relation to antioxidant properties from Fortunella polyandra
title_sort	chemical profiling and optimization of rhoifolin extraction in relation to antioxidant properties from fortunella polyandra
granting_institution	Universiti Teknologi Malaysia
granting_department	Faculty of Science
publishDate	2021
url	http://eprints.utm.my/id/eprint/101943/1/HazimSyahmiEliasMFS2021.pdf
_version_	1776100808973090816

Chemical profiling and optimization of rhoifolin extraction in relation to antioxidant properties from Fortunella polyandra

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