Eco-friendly bacterial prodigiosin-based antibiofouling coating for marine structure applications

Marine biofouling, the unwanted accumulation of living organisms on manmade structures is worsening and creating an adverse economic impact. In this study, a more sustainable antibiofouling paint was formulated by using natural antibiofouling agent. Prodigiosin, a bacterial pigment extracted from Se...

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Main Author:	Amir, Siti Nabilah
Format:	Thesis
Language:	English
Published:	2020
Subjects:	QD Chemistry
Online Access:	http://eprints.utm.my/id/eprint/102236/1/SitiNabilahAmirMFS2020.pdf.pdf
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id	my-utm-ep.102236
record_format	uketd_dc
spelling	my-utm-ep.1022362023-08-13T06:10:47Z Eco-friendly bacterial prodigiosin-based antibiofouling coating for marine structure applications 2020 Amir, Siti Nabilah QD Chemistry Marine biofouling, the unwanted accumulation of living organisms on manmade structures is worsening and creating an adverse economic impact. In this study, a more sustainable antibiofouling paint was formulated by using natural antibiofouling agent. Prodigiosin, a bacterial pigment extracted from Serratia marcescens was used as the antibiofouling agent and pigment. The antibiofouling properties of crude prodigiosin were studied by assessing the antibacterial properties, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and toxicity assay. The performance of antibiofouling paint at different pigment composition incorporated with crude prodigiosin was studied in laboratory and in natural seawater. Based on the result obtained, the antibacterial test demonstrated the largest inhibition zone against B. subtilis (4.0 ± 0.3 cm) at 10000 ppm and the smallest inhibition zone against E. coli (2.0 ± 0.4 cm) at 1000 ppm. The MIC values ranged from 390 to 3126 ppm and MBC values ranged from 1562 ppm to 6250 ppm for all bacteria tested. Crude prodigiosin showed a low toxicity value (LC50 = 4270.203 ppm). The comparative study of binder showed that vinyl ester resin performed better in comparison to epoxy resin in all tests, namely antibacterial test (B. subtilis: 3.77 ± 0.1 cm, E. coli: 3.43 ± 0.1, P. aeruginosa: 3.63 ± 0.3, S. aureus: 4.00 ± 0.1), surface roughness and adhesion test (Class 5B). Steel plate painted with crude prodigiosin was able to retain its cleanliness at high flowrate areas (significant, p = 0.05) in natural seawater while a thinner mud settlement was observed at low flowrate areas (not significant, p = 0.05). Based on the research outcomes, it was observed that with thorough study, prodigiosin has a high potential as an effective environmental friendly antibiofouling agent since it can inhibit the attachment of marine organisms on wetted surfaces. 2020 Thesis http://eprints.utm.my/id/eprint/102236/ http://eprints.utm.my/id/eprint/102236/1/SitiNabilahAmirMFS2020.pdf.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:146295 masters Universiti Teknologi Malaysia Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Amir, Siti Nabilah Eco-friendly bacterial prodigiosin-based antibiofouling coating for marine structure applications
description	Marine biofouling, the unwanted accumulation of living organisms on manmade structures is worsening and creating an adverse economic impact. In this study, a more sustainable antibiofouling paint was formulated by using natural antibiofouling agent. Prodigiosin, a bacterial pigment extracted from Serratia marcescens was used as the antibiofouling agent and pigment. The antibiofouling properties of crude prodigiosin were studied by assessing the antibacterial properties, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and toxicity assay. The performance of antibiofouling paint at different pigment composition incorporated with crude prodigiosin was studied in laboratory and in natural seawater. Based on the result obtained, the antibacterial test demonstrated the largest inhibition zone against B. subtilis (4.0 ± 0.3 cm) at 10000 ppm and the smallest inhibition zone against E. coli (2.0 ± 0.4 cm) at 1000 ppm. The MIC values ranged from 390 to 3126 ppm and MBC values ranged from 1562 ppm to 6250 ppm for all bacteria tested. Crude prodigiosin showed a low toxicity value (LC50 = 4270.203 ppm). The comparative study of binder showed that vinyl ester resin performed better in comparison to epoxy resin in all tests, namely antibacterial test (B. subtilis: 3.77 ± 0.1 cm, E. coli: 3.43 ± 0.1, P. aeruginosa: 3.63 ± 0.3, S. aureus: 4.00 ± 0.1), surface roughness and adhesion test (Class 5B). Steel plate painted with crude prodigiosin was able to retain its cleanliness at high flowrate areas (significant, p = 0.05) in natural seawater while a thinner mud settlement was observed at low flowrate areas (not significant, p = 0.05). Based on the research outcomes, it was observed that with thorough study, prodigiosin has a high potential as an effective environmental friendly antibiofouling agent since it can inhibit the attachment of marine organisms on wetted surfaces.
format	Thesis
qualification_level	Master's degree
author	Amir, Siti Nabilah
author_facet	Amir, Siti Nabilah
author_sort	Amir, Siti Nabilah
title	Eco-friendly bacterial prodigiosin-based antibiofouling coating for marine structure applications
title_short	Eco-friendly bacterial prodigiosin-based antibiofouling coating for marine structure applications
title_full	Eco-friendly bacterial prodigiosin-based antibiofouling coating for marine structure applications
title_fullStr	Eco-friendly bacterial prodigiosin-based antibiofouling coating for marine structure applications
title_full_unstemmed	Eco-friendly bacterial prodigiosin-based antibiofouling coating for marine structure applications
title_sort	eco-friendly bacterial prodigiosin-based antibiofouling coating for marine structure applications
granting_institution	Universiti Teknologi Malaysia
granting_department	Faculty of Science
publishDate	2020
url	http://eprints.utm.my/id/eprint/102236/1/SitiNabilahAmirMFS2020.pdf.pdf
_version_	1776100875722293248

Eco-friendly bacterial prodigiosin-based antibiofouling coating for marine structure applications

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