Extraction and characterization of purple pigment from chromobacterium violaceum grown in agricultural wastes

There has been an increasing trend towards replacement of synthetic colorants with natural pigments in last decades because of the strong consumer demand for more natural products. Among three groups of main natural pigments, bacterial pigments are considered as an alternative to synthesized dye. Pr...

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Main Author:	Neshati, Akram
Format:	Thesis
Language:	English
Published:	2010
Subjects:	QD Chemistry
Online Access:	http://eprints.utm.my/id/eprint/11243/6/AkramNeshatiMFS2010.pdf
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id	my-utm-ep.11243
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spelling	my-utm-ep.112432017-09-28T01:17:09Z Extraction and characterization of purple pigment from chromobacterium violaceum grown in agricultural wastes 2010-04 Neshati, Akram QD Chemistry There has been an increasing trend towards replacement of synthetic colorants with natural pigments in last decades because of the strong consumer demand for more natural products. Among three groups of main natural pigments, bacterial pigments are considered as an alternative to synthesized dye. Production and extraction of violet pigment of Chromobacterium violaceum grown on agricultural waste such as solid pineapple waste (SPW) and brown sugar (BS) was studied. From the study, the optimum growth temperature of C. violaceum and pigment production is at 25°C and optimum pH is 7. The pigment was extracted from the growth media using two solvents which were methanol and ethyl acetate. Characterization of the purple pigment was carried out using UV-VIS spectrophotometer, FTIR and 1H and 13C-NMR. UV-VIS analysis of the purple pigment samples from nutrient broth (NB), BS and SPW media shows ?max at 566.50, 567.50 and 571.94 nm respectively. FTIR spectrum of purple pigment pellet from BS growth medium showed a broad peak at 3430.10 cm-1 assigned to OH stretching, overlapping of N-H bond with O-H stretching observed at 3330.1 cm-1, two stretching bonds at 1640 cm-1 and 1723.5 cm-1 assigned to the C=O amide groups and C=C peak at 1615.92 cm-1. 1H-CNMR and 13C-NMR spectra were recorded in DMSO-d6 and 20 carbon peaks and also 13 proton peaks appeared in the result to confirm the present of violacein in the samples. Lastly, stability of the produced pigment towards changes of the pH was examined. The pigment shows different colors at different pH. 2010-04 Thesis http://eprints.utm.my/id/eprint/11243/ http://eprints.utm.my/id/eprint/11243/6/AkramNeshatiMFS2010.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Science Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Neshati, Akram Extraction and characterization of purple pigment from chromobacterium violaceum grown in agricultural wastes
description	There has been an increasing trend towards replacement of synthetic colorants with natural pigments in last decades because of the strong consumer demand for more natural products. Among three groups of main natural pigments, bacterial pigments are considered as an alternative to synthesized dye. Production and extraction of violet pigment of Chromobacterium violaceum grown on agricultural waste such as solid pineapple waste (SPW) and brown sugar (BS) was studied. From the study, the optimum growth temperature of C. violaceum and pigment production is at 25°C and optimum pH is 7. The pigment was extracted from the growth media using two solvents which were methanol and ethyl acetate. Characterization of the purple pigment was carried out using UV-VIS spectrophotometer, FTIR and 1H and 13C-NMR. UV-VIS analysis of the purple pigment samples from nutrient broth (NB), BS and SPW media shows ?max at 566.50, 567.50 and 571.94 nm respectively. FTIR spectrum of purple pigment pellet from BS growth medium showed a broad peak at 3430.10 cm-1 assigned to OH stretching, overlapping of N-H bond with O-H stretching observed at 3330.1 cm-1, two stretching bonds at 1640 cm-1 and 1723.5 cm-1 assigned to the C=O amide groups and C=C peak at 1615.92 cm-1. 1H-CNMR and 13C-NMR spectra were recorded in DMSO-d6 and 20 carbon peaks and also 13 proton peaks appeared in the result to confirm the present of violacein in the samples. Lastly, stability of the produced pigment towards changes of the pH was examined. The pigment shows different colors at different pH.
format	Thesis
qualification_level	Master's degree
author	Neshati, Akram
author_facet	Neshati, Akram
author_sort	Neshati, Akram
title	Extraction and characterization of purple pigment from chromobacterium violaceum grown in agricultural wastes
title_short	Extraction and characterization of purple pigment from chromobacterium violaceum grown in agricultural wastes
title_full	Extraction and characterization of purple pigment from chromobacterium violaceum grown in agricultural wastes
title_fullStr	Extraction and characterization of purple pigment from chromobacterium violaceum grown in agricultural wastes
title_full_unstemmed	Extraction and characterization of purple pigment from chromobacterium violaceum grown in agricultural wastes
title_sort	extraction and characterization of purple pigment from chromobacterium violaceum grown in agricultural wastes
granting_institution	Universiti Teknologi Malaysia, Faculty of Science
granting_department	Faculty of Science
publishDate	2010
url	http://eprints.utm.my/id/eprint/11243/6/AkramNeshatiMFS2010.pdf
_version_	1747814832055255040

Extraction and characterization of purple pigment from chromobacterium violaceum grown in agricultural wastes

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