A digital signal processing approach to analyze gel electrophoresis image

Gel Electrophoresis (GE) is a widely used technique to separate DNA according to their size and weight, generates images that can be analyzed automatically. The separated DNA fragments or proteins of different molecular weights will give a series of bands with positions corresponding to the molecula...

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Main Author:	Abdullah, Noor Ezan
Format:	Thesis
Language:	English
Published:	2009
Subjects:	TK Electrical engineering Electronics Nuclear engineering
Online Access:	http://eprints.utm.my/id/eprint/12001/6/NoorEzanAbdullahMFKE2009.pdf
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id	my-utm-ep.12001
record_format	uketd_dc
spelling	my-utm-ep.120012017-09-14T01:27:57Z A digital signal processing approach to analyze gel electrophoresis image 2009-11 Abdullah, Noor Ezan TK Electrical engineering. Electronics Nuclear engineering Gel Electrophoresis (GE) is a widely used technique to separate DNA according to their size and weight, generates images that can be analyzed automatically. The separated DNA fragments or proteins of different molecular weights will give a series of bands with positions corresponding to the molecular weight. Image analysis of the gels removes much of the subjectivity of manual comparison of band position and intensity between samples. Briefly, this project presents a semiautomatic image processing techniques attempts to detect lanes, bands and length or size estimation of the bands. In this project, the routines are fully written in MATLAB R2008a. This project consists of three stages. The first stage is pre-processing, which involves conversion of RGB image into greyscale image. The second stage is to identify the number of lane in the image including the lane for marker and also to distinguish between the lane of marker and unknown DNA samples. The final stage is the identification of the length of DNA molecules each band in the lane based on data provided by the DNA marker. 2009-11 Thesis http://eprints.utm.my/id/eprint/12001/ http://eprints.utm.my/id/eprint/12001/6/NoorEzanAbdullahMFKE2009.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Electrical Engineering Faculty of Electrical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TK Electrical engineering Electronics Nuclear engineering
spellingShingle	TK Electrical engineering Electronics Nuclear engineering Abdullah, Noor Ezan A digital signal processing approach to analyze gel electrophoresis image
description	Gel Electrophoresis (GE) is a widely used technique to separate DNA according to their size and weight, generates images that can be analyzed automatically. The separated DNA fragments or proteins of different molecular weights will give a series of bands with positions corresponding to the molecular weight. Image analysis of the gels removes much of the subjectivity of manual comparison of band position and intensity between samples. Briefly, this project presents a semiautomatic image processing techniques attempts to detect lanes, bands and length or size estimation of the bands. In this project, the routines are fully written in MATLAB R2008a. This project consists of three stages. The first stage is pre-processing, which involves conversion of RGB image into greyscale image. The second stage is to identify the number of lane in the image including the lane for marker and also to distinguish between the lane of marker and unknown DNA samples. The final stage is the identification of the length of DNA molecules each band in the lane based on data provided by the DNA marker.
format	Thesis
qualification_level	Master's degree
author	Abdullah, Noor Ezan
author_facet	Abdullah, Noor Ezan
author_sort	Abdullah, Noor Ezan
title	A digital signal processing approach to analyze gel electrophoresis image
title_short	A digital signal processing approach to analyze gel electrophoresis image
title_full	A digital signal processing approach to analyze gel electrophoresis image
title_fullStr	A digital signal processing approach to analyze gel electrophoresis image
title_full_unstemmed	A digital signal processing approach to analyze gel electrophoresis image
title_sort	digital signal processing approach to analyze gel electrophoresis image
granting_institution	Universiti Teknologi Malaysia, Faculty of Electrical Engineering
granting_department	Faculty of Electrical Engineering
publishDate	2009
url	http://eprints.utm.my/id/eprint/12001/6/NoorEzanAbdullahMFKE2009.pdf
_version_	1747814885135220736

A digital signal processing approach to analyze gel electrophoresis image

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