Cloning of influenza B NS1 gene in Escherichia Coli
The non-structural NS1 protein of influenza B virus is a multifunctional virulence protein which is involved in the transport of viral RNA. Inside the host cell, NS1B antagonizes and inhibits the a/ß interferon system which is induced as host antiviral response. Moreover, it prevents the activation...
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2010
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my-utm-ep.122832017-09-20T04:08:49Z Cloning of influenza B NS1 gene in Escherichia Coli 2010-11 Adel Dawood, Ali RZ Other systems of medicine The non-structural NS1 protein of influenza B virus is a multifunctional virulence protein which is involved in the transport of viral RNA. Inside the host cell, NS1B antagonizes and inhibits the a/ß interferon system which is induced as host antiviral response. Moreover, it prevents the activation of double-stranded-RNA activated protein kinase (PKR) by binding to dsRNA and inhibits the maturation of GAS8 (gene of tumor suppressor). NS1 protein is utilized as a target for diagnostic of influenza viruses in infected animals. pUC57 carrying NS1 synthetic gene of influenza B was attempted to be transformed into Escherichia coli strain BL21(DE3). NS1B was extracted and attempted to be cloned into prokaryotic expression vectors pET-32b, pET-32a, pQE-81L and pQE-80L, respectively using restriction digestion enzymes (SacI, PstI and HindIII). Then, recombinant DNA was attempted to be transformed into Escherichia coli strains BL21(DE3) and DH5a. 2010-11 Thesis http://eprints.utm.my/id/eprint/12283/ http://eprints.utm.my/id/eprint/12283/4/AliAdelDawoodMFBB2010.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Biosciences and Bioengineering Faculty of Biosciences and Bioengineering |
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English |
topic |
RZ Other systems of medicine |
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RZ Other systems of medicine Adel Dawood, Ali Cloning of influenza B NS1 gene in Escherichia Coli |
description |
The non-structural NS1 protein of influenza B virus is a multifunctional virulence protein which is involved in the transport of viral RNA. Inside the host cell, NS1B antagonizes and inhibits the a/ß interferon system which is induced as host antiviral response. Moreover, it prevents the activation of double-stranded-RNA activated protein kinase (PKR) by binding to dsRNA and inhibits the maturation of GAS8 (gene of tumor suppressor). NS1 protein is utilized as a target for diagnostic of influenza viruses in infected animals. pUC57 carrying NS1 synthetic gene of influenza B was attempted to be transformed into Escherichia coli strain BL21(DE3). NS1B was extracted and attempted to be cloned into prokaryotic expression vectors pET-32b, pET-32a, pQE-81L and pQE-80L, respectively using restriction digestion enzymes (SacI, PstI and HindIII). Then, recombinant DNA was attempted to be transformed into Escherichia coli strains BL21(DE3) and DH5a. |
format |
Thesis |
qualification_level |
Master's degree |
author |
Adel Dawood, Ali |
author_facet |
Adel Dawood, Ali |
author_sort |
Adel Dawood, Ali |
title |
Cloning of influenza B NS1 gene in Escherichia Coli |
title_short |
Cloning of influenza B NS1 gene in Escherichia Coli |
title_full |
Cloning of influenza B NS1 gene in Escherichia Coli |
title_fullStr |
Cloning of influenza B NS1 gene in Escherichia Coli |
title_full_unstemmed |
Cloning of influenza B NS1 gene in Escherichia Coli |
title_sort |
cloning of influenza b ns1 gene in escherichia coli |
granting_institution |
Universiti Teknologi Malaysia, Faculty of Biosciences and Bioengineering |
granting_department |
Faculty of Biosciences and Bioengineering |
publishDate |
2010 |
url |
http://eprints.utm.my/id/eprint/12283/4/AliAdelDawoodMFBB2010.pdf |
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1747814913804337152 |