Cloning of influenza B NS1 gene in Escherichia Coli

The non-structural NS1 protein of influenza B virus is a multifunctional virulence protein which is involved in the transport of viral RNA. Inside the host cell, NS1B antagonizes and inhibits the a/ß interferon system which is induced as host antiviral response. Moreover, it prevents the activation...

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Main Author:	Adel Dawood, Ali
Format:	Thesis
Language:	English
Published:	2010
Subjects:	RZ Other systems of medicine
Online Access:	http://eprints.utm.my/id/eprint/12283/4/AliAdelDawoodMFBB2010.pdf
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id	my-utm-ep.12283
record_format	uketd_dc
spelling	my-utm-ep.122832017-09-20T04:08:49Z Cloning of influenza B NS1 gene in Escherichia Coli 2010-11 Adel Dawood, Ali RZ Other systems of medicine The non-structural NS1 protein of influenza B virus is a multifunctional virulence protein which is involved in the transport of viral RNA. Inside the host cell, NS1B antagonizes and inhibits the a/ß interferon system which is induced as host antiviral response. Moreover, it prevents the activation of double-stranded-RNA activated protein kinase (PKR) by binding to dsRNA and inhibits the maturation of GAS8 (gene of tumor suppressor). NS1 protein is utilized as a target for diagnostic of influenza viruses in infected animals. pUC57 carrying NS1 synthetic gene of influenza B was attempted to be transformed into Escherichia coli strain BL21(DE3). NS1B was extracted and attempted to be cloned into prokaryotic expression vectors pET-32b, pET-32a, pQE-81L and pQE-80L, respectively using restriction digestion enzymes (SacI, PstI and HindIII). Then, recombinant DNA was attempted to be transformed into Escherichia coli strains BL21(DE3) and DH5a. 2010-11 Thesis http://eprints.utm.my/id/eprint/12283/ http://eprints.utm.my/id/eprint/12283/4/AliAdelDawoodMFBB2010.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Biosciences and Bioengineering Faculty of Biosciences and Bioengineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	RZ Other systems of medicine
spellingShingle	RZ Other systems of medicine Adel Dawood, Ali Cloning of influenza B NS1 gene in Escherichia Coli
description	The non-structural NS1 protein of influenza B virus is a multifunctional virulence protein which is involved in the transport of viral RNA. Inside the host cell, NS1B antagonizes and inhibits the a/ß interferon system which is induced as host antiviral response. Moreover, it prevents the activation of double-stranded-RNA activated protein kinase (PKR) by binding to dsRNA and inhibits the maturation of GAS8 (gene of tumor suppressor). NS1 protein is utilized as a target for diagnostic of influenza viruses in infected animals. pUC57 carrying NS1 synthetic gene of influenza B was attempted to be transformed into Escherichia coli strain BL21(DE3). NS1B was extracted and attempted to be cloned into prokaryotic expression vectors pET-32b, pET-32a, pQE-81L and pQE-80L, respectively using restriction digestion enzymes (SacI, PstI and HindIII). Then, recombinant DNA was attempted to be transformed into Escherichia coli strains BL21(DE3) and DH5a.
format	Thesis
qualification_level	Master's degree
author	Adel Dawood, Ali
author_facet	Adel Dawood, Ali
author_sort	Adel Dawood, Ali
title	Cloning of influenza B NS1 gene in Escherichia Coli
title_short	Cloning of influenza B NS1 gene in Escherichia Coli
title_full	Cloning of influenza B NS1 gene in Escherichia Coli
title_fullStr	Cloning of influenza B NS1 gene in Escherichia Coli
title_full_unstemmed	Cloning of influenza B NS1 gene in Escherichia Coli
title_sort	cloning of influenza b ns1 gene in escherichia coli
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Bioengineering
granting_department	Faculty of Biosciences and Bioengineering
publishDate	2010
url	http://eprints.utm.my/id/eprint/12283/4/AliAdelDawoodMFBB2010.pdf
_version_	1747814913804337152

Cloning of influenza B NS1 gene in Escherichia Coli

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