Isolation and cloning of human NQO1 promoter in pGL3 basic vector

Malaria is a major public health problem caused by Plasmodium falciparum, a parasite that infects red blood cells. Recently, several polyphenolic compounds have been reported capable of preventing the progression of malaria parasite. This observation may be related to NAD (P) H: quinon oxidoreductas...

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Main Author:	Chinigarzadeh, Asma
Format:	Thesis
Published:	2010
Subjects:	RZ Other systems of medicine
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id	my-utm-ep.12287
record_format	uketd_dc
spelling	my-utm-ep.122872011-04-14T09:44:43Z Isolation and cloning of human NQO1 promoter in pGL3 basic vector 2010-12 Chinigarzadeh, Asma RZ Other systems of medicine Malaria is a major public health problem caused by Plasmodium falciparum, a parasite that infects red blood cells. Recently, several polyphenolic compounds have been reported capable of preventing the progression of malaria parasite. This observation may be related to NAD (P) H: quinon oxidoreductase: a flavoprotein responsible for catalyzing two-electron reduction and detoxification of quinones and their derivatives. In this study the 2123 bp of the 5` upstream of the first transcription start site was successfully isolated. Based on the bioinformatic program, several regulatory regions such as Antioxidant Response Element (ARE) may be responsible for the direct regulation of polyphenols on this enzyme. It was predicted at -477 from the first transcription start site. Upon isolation, this fragment was used in a cloning process into the pGL3 Basic vector and transformed to E.coli DH5? competent cells 2010-12 Thesis http://eprints.utm.my/id/eprint/12287/ masters Universiti Teknologi Malaysia, Faculty of Bioscience and Bioengineering Faculty of Bioscience and Bioengineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
topic	RZ Other systems of medicine
spellingShingle	RZ Other systems of medicine Chinigarzadeh, Asma Isolation and cloning of human NQO1 promoter in pGL3 basic vector
description	Malaria is a major public health problem caused by Plasmodium falciparum, a parasite that infects red blood cells. Recently, several polyphenolic compounds have been reported capable of preventing the progression of malaria parasite. This observation may be related to NAD (P) H: quinon oxidoreductase: a flavoprotein responsible for catalyzing two-electron reduction and detoxification of quinones and their derivatives. In this study the 2123 bp of the 5` upstream of the first transcription start site was successfully isolated. Based on the bioinformatic program, several regulatory regions such as Antioxidant Response Element (ARE) may be responsible for the direct regulation of polyphenols on this enzyme. It was predicted at -477 from the first transcription start site. Upon isolation, this fragment was used in a cloning process into the pGL3 Basic vector and transformed to E.coli DH5? competent cells
format	Thesis
qualification_level	Master's degree
author	Chinigarzadeh, Asma
author_facet	Chinigarzadeh, Asma
author_sort	Chinigarzadeh, Asma
title	Isolation and cloning of human NQO1 promoter in pGL3 basic vector
title_short	Isolation and cloning of human NQO1 promoter in pGL3 basic vector
title_full	Isolation and cloning of human NQO1 promoter in pGL3 basic vector
title_fullStr	Isolation and cloning of human NQO1 promoter in pGL3 basic vector
title_full_unstemmed	Isolation and cloning of human NQO1 promoter in pGL3 basic vector
title_sort	isolation and cloning of human nqo1 promoter in pgl3 basic vector
granting_institution	Universiti Teknologi Malaysia, Faculty of Bioscience and Bioengineering
granting_department	Faculty of Bioscience and Bioengineering
publishDate	2010
_version_	1747814914781609984

Isolation and cloning of human NQO1 promoter in pGL3 basic vector

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