Accelerated solvent extraction of bioactive compounds (Sinensetin) from orthosiphon stamineous and optimization of process parameters

Herbal medicine plays an important part of health care to a majority of the world’s population. Orthosiphon Stamineus(Benth.) used in southeast Asia for treating various diseases. An accelerated solvent extraction (ASE) method was developed to extract interest compounds from crude extract of Orthosi...

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Main Author:	Pouralinazar, Farzad
Format:	Thesis
Published:	2010
Subjects:	QD Chemistry
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id	my-utm-ep.16264
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spelling	my-utm-ep.162642017-08-17T02:41:46Z Accelerated solvent extraction of bioactive compounds (Sinensetin) from orthosiphon stamineous and optimization of process parameters 2010 Pouralinazar, Farzad QD Chemistry Herbal medicine plays an important part of health care to a majority of the world’s population. Orthosiphon Stamineus(Benth.) used in southeast Asia for treating various diseases. An accelerated solvent extraction (ASE) method was developed to extract interest compounds from crude extract of Orthosiphon Stamineus(Benth).ln this study, the interest compound selected is Sinensetin, which is used as anti-cancer agent. The layout of experiment was developed via Statistica 8.0using the Box-Behnken experimental design model. The oil yield and quantification of interest compounds were calculated and the response surface methodology (RSM) was done to compare the result from the experiment and from the statistic. Through the RSM the optimum condition for the extraction process from the Orthosiphon Stamineus crude extract was determined. It was revealed that the value from the model equation was in good agreement with the experimental values with the regression coefficient, RA2, 0.9863.F test also indicated that the model chosen is suitable. 100°C, lOmin and 2extraction cycles were found to be the best and optimum conditions. In other words, highest oil yield, 22.75%, was found in mentioned optimum conditions. HPLC was used to determine concentration of bioactive compound (Sinensetin); however, concentration of SEN was almost negligible in this study. Ethanol was selected as extraction solvent and further investigation is needed to find what GRAS solvent is suitable to extract SEN from orthosiphun staminues. 2010 Thesis http://eprints.utm.my/id/eprint/16264/ masters Universiti Teknologi Malaysia, Faculty of Chemical Engineering Faculty of Chemical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
topic	QD Chemistry
spellingShingle	QD Chemistry Pouralinazar, Farzad Accelerated solvent extraction of bioactive compounds (Sinensetin) from orthosiphon stamineous and optimization of process parameters
description	Herbal medicine plays an important part of health care to a majority of the world’s population. Orthosiphon Stamineus(Benth.) used in southeast Asia for treating various diseases. An accelerated solvent extraction (ASE) method was developed to extract interest compounds from crude extract of Orthosiphon Stamineus(Benth).ln this study, the interest compound selected is Sinensetin, which is used as anti-cancer agent. The layout of experiment was developed via Statistica 8.0using the Box-Behnken experimental design model. The oil yield and quantification of interest compounds were calculated and the response surface methodology (RSM) was done to compare the result from the experiment and from the statistic. Through the RSM the optimum condition for the extraction process from the Orthosiphon Stamineus crude extract was determined. It was revealed that the value from the model equation was in good agreement with the experimental values with the regression coefficient, RA2, 0.9863.F test also indicated that the model chosen is suitable. 100°C, lOmin and 2extraction cycles were found to be the best and optimum conditions. In other words, highest oil yield, 22.75%, was found in mentioned optimum conditions. HPLC was used to determine concentration of bioactive compound (Sinensetin); however, concentration of SEN was almost negligible in this study. Ethanol was selected as extraction solvent and further investigation is needed to find what GRAS solvent is suitable to extract SEN from orthosiphun staminues.
format	Thesis
qualification_level	Master's degree
author	Pouralinazar, Farzad
author_facet	Pouralinazar, Farzad
author_sort	Pouralinazar, Farzad
title	Accelerated solvent extraction of bioactive compounds (Sinensetin) from orthosiphon stamineous and optimization of process parameters
title_short	Accelerated solvent extraction of bioactive compounds (Sinensetin) from orthosiphon stamineous and optimization of process parameters
title_full	Accelerated solvent extraction of bioactive compounds (Sinensetin) from orthosiphon stamineous and optimization of process parameters
title_fullStr	Accelerated solvent extraction of bioactive compounds (Sinensetin) from orthosiphon stamineous and optimization of process parameters
title_full_unstemmed	Accelerated solvent extraction of bioactive compounds (Sinensetin) from orthosiphon stamineous and optimization of process parameters
title_sort	accelerated solvent extraction of bioactive compounds (sinensetin) from orthosiphon stamineous and optimization of process parameters
granting_institution	Universiti Teknologi Malaysia, Faculty of Chemical Engineering
granting_department	Faculty of Chemical Engineering
publishDate	2010
_version_	1747815001240895488

Accelerated solvent extraction of bioactive compounds (Sinensetin) from orthosiphon stamineous and optimization of process parameters

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