In vitro plant regenaration of tobacco (nicotiana tabaccum TAPM 26)

In vitro plant regenaration of tobacco (nicotiana tabaccum TAPM 26)

In the present research, a reproducible procedure has been developed for efficient plant regeneration of shoot from cotyledon explants of Nicotiana tabaccum TAPM 26. In vitro regeneration of Nicotiana tabaccum TAPM 26 was efficiently achieved applying combination of growth hormones supplemented into...

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Main Author: Kaya, Yilmaz
Format: Thesis
Language:English
Published: 2010
Subjects:
Q Science (General)
SB Plant culture
Online Access:http://eprints.utm.my/id/eprint/16470/1/YilmazKayaMFBB2010.pdf
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spelling my-utm-ep.164702017-09-13T00:44:56Z In vitro plant regenaration of tobacco (nicotiana tabaccum TAPM 26) 2010 Kaya, Yilmaz Q Science (General) SB Plant culture In the present research, a reproducible procedure has been developed for efficient plant regeneration of shoot from cotyledon explants of Nicotiana tabaccum TAPM 26. In vitro regeneration of Nicotiana tabaccum TAPM 26 was efficiently achieved applying combination of growth hormones supplemented into MS medium using cotyledon from 5-day-old and 7-day-old as explants. The growth hormones used were IAA for auxin (0.0, 0.1, 1, 10 mgL-1) and BAP for cytokinin (0.0, 0.1, 1, 10 mgL-1 ). The effects of growth hormones combination were assessed based on number of shoot and roots formation. Maximum number of shoots produced per explant was found when media continued with 10 µM BAP +0.0 µM IAA after 4 weeks in culture. Maximum number of root produced per explant was reached when media continued 10 µM IAA after 4 weeks in MS media. The current techniques proved that Nicotiana tabaccum was successfully propagated by direct organogenesis and can be applied for further research in making as transgenic Nicotiana tabaccum. 2010 Thesis http://eprints.utm.my/id/eprint/16470/ http://eprints.utm.my/id/eprint/16470/1/YilmazKayaMFBB2010.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Biosciences and Bioengineering Faculty of Biosciences and Bioengineering
institution Universiti Teknologi Malaysia
collection UTM Institutional Repository
language English
topic Q Science (General)
SB Plant culture
spellingShingle Q Science (General)
SB Plant culture
Kaya, Yilmaz
In vitro plant regenaration of tobacco (nicotiana tabaccum TAPM 26)
description In the present research, a reproducible procedure has been developed for efficient plant regeneration of shoot from cotyledon explants of Nicotiana tabaccum TAPM 26. In vitro regeneration of Nicotiana tabaccum TAPM 26 was efficiently achieved applying combination of growth hormones supplemented into MS medium using cotyledon from 5-day-old and 7-day-old as explants. The growth hormones used were IAA for auxin (0.0, 0.1, 1, 10 mgL-1) and BAP for cytokinin (0.0, 0.1, 1, 10 mgL-1 ). The effects of growth hormones combination were assessed based on number of shoot and roots formation. Maximum number of shoots produced per explant was found when media continued with 10 µM BAP +0.0 µM IAA after 4 weeks in culture. Maximum number of root produced per explant was reached when media continued 10 µM IAA after 4 weeks in MS media. The current techniques proved that Nicotiana tabaccum was successfully propagated by direct organogenesis and can be applied for further research in making as transgenic Nicotiana tabaccum.
format Thesis
qualification_level Master's degree
author Kaya, Yilmaz
author_facet Kaya, Yilmaz
author_sort Kaya, Yilmaz
title In vitro plant regenaration of tobacco (nicotiana tabaccum TAPM 26)
title_short In vitro plant regenaration of tobacco (nicotiana tabaccum TAPM 26)
title_full In vitro plant regenaration of tobacco (nicotiana tabaccum TAPM 26)
title_fullStr In vitro plant regenaration of tobacco (nicotiana tabaccum TAPM 26)
title_full_unstemmed In vitro plant regenaration of tobacco (nicotiana tabaccum TAPM 26)
title_sort in vitro plant regenaration of tobacco (nicotiana tabaccum tapm 26)
granting_institution Universiti Teknologi Malaysia, Faculty of Biosciences and Bioengineering
granting_department Faculty of Biosciences and Bioengineering
publishDate 2010
url http://eprints.utm.my/id/eprint/16470/1/YilmazKayaMFBB2010.pdf
_version_ 1747815050600513536

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