Isolation and cloning of human VEGF promoter region in PGL3 basic vector
Worldwide mortality and morbidity from infectious diseases is being replaced by chronic diseases, such as cancer, obesity and type II diabetes, cardiovascular diseases, neurodegenerative diseases and aging which involve inflammation. Vascular endothelial growth factor (VEGF) as a potent pro-inflamma...
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2011
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my-utm-ep.180982018-05-27T03:24:29Z Isolation and cloning of human VEGF promoter region in PGL3 basic vector 2011-05 Yaze, Iman Q Science (General) Worldwide mortality and morbidity from infectious diseases is being replaced by chronic diseases, such as cancer, obesity and type II diabetes, cardiovascular diseases, neurodegenerative diseases and aging which involve inflammation. Vascular endothelial growth factor (VEGF) as a potent pro-inflammatory cytokine is elevated in many human diseases, or animal models of human disease, which are mentioned above. Compounds derived from botanic sources, such as polyphenolic compounds, have shown anti-inflammatory activity. These natural compounds express anti-inflammatory activity by modulation of pro-inflammatory gene expression. We hypothesized this effect may related to control regulation of VEGF gene promoter. In this study, 5` flanking region of the VEGF promoter were isolated using nested PCR to define the transcription factors binding sites. The human VEGF promoter region was cloned in DH5 alpha for future investigation 2011-05 Thesis http://eprints.utm.my/id/eprint/18098/ http://eprints.utm.my/id/eprint/18098/1/ImanYazeMFBME2011.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:83103?queryType=vitalDismax&query=Isolation+and+cloning+of+human+VEGF+promoter+region+in+PGL3+basic+vector&public=true masters Universiti Teknologi Malaysia, Faculty of Bioscience and Bioengineering Faculty of Bioscience and Bioengineering |
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Universiti Teknologi Malaysia |
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UTM Institutional Repository |
| language |
English |
| topic |
Q Science (General) |
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Q Science (General) Yaze, Iman Isolation and cloning of human VEGF promoter region in PGL3 basic vector |
| description |
Worldwide mortality and morbidity from infectious diseases is being replaced by chronic diseases, such as cancer, obesity and type II diabetes, cardiovascular diseases, neurodegenerative diseases and aging which involve inflammation. Vascular endothelial growth factor (VEGF) as a potent pro-inflammatory cytokine is elevated in many human diseases, or animal models of human disease, which are mentioned above. Compounds derived from botanic sources, such as polyphenolic compounds, have shown anti-inflammatory activity. These natural compounds express anti-inflammatory activity by modulation of pro-inflammatory gene expression. We hypothesized this effect may related to control regulation of VEGF gene promoter. In this study, 5` flanking region of the VEGF promoter were isolated using nested PCR to define the transcription factors binding sites. The human VEGF promoter region was cloned in DH5 alpha for future investigation |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Yaze, Iman |
| author_facet |
Yaze, Iman |
| author_sort |
Yaze, Iman |
| title |
Isolation and cloning of human VEGF promoter region in PGL3 basic vector |
| title_short |
Isolation and cloning of human VEGF promoter region in PGL3 basic vector |
| title_full |
Isolation and cloning of human VEGF promoter region in PGL3 basic vector |
| title_fullStr |
Isolation and cloning of human VEGF promoter region in PGL3 basic vector |
| title_full_unstemmed |
Isolation and cloning of human VEGF promoter region in PGL3 basic vector |
| title_sort |
isolation and cloning of human vegf promoter region in pgl3 basic vector |
| granting_institution |
Universiti Teknologi Malaysia, Faculty of Bioscience and Bioengineering |
| granting_department |
Faculty of Bioscience and Bioengineering |
| publishDate |
2011 |
| url |
http://eprints.utm.my/id/eprint/18098/1/ImanYazeMFBME2011.pdf |
| _version_ |
1747815192200216576 |