Plant protein hydrolysates as a supplement for medium of human skin fibroblast 1184 cell culture

Medium is the most important item in cell culture. Each medium consists of two main parts which are a basal medium and serum. The main source of the serum is come from an animal blood. The serum from animal blood has many disadvantages such as viral contaminations. Moreover, serum is very expensive....

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Main Author:	Kazemzadeh Farizhandi, Amir Abbas
Format:	Thesis
Language:	English
Published:	2012
Subjects:	Q Science (General)
Online Access:	http://eprints.utm.my/id/eprint/32433/1/AmirAbbasKazemzadehFarizhandiMFCE2012.pdf
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id	my-utm-ep.32433
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spelling	my-utm-ep.324332017-08-09T08:22:31Z Plant protein hydrolysates as a supplement for medium of human skin fibroblast 1184 cell culture 2012 Kazemzadeh Farizhandi, Amir Abbas Q Science (General) Medium is the most important item in cell culture. Each medium consists of two main parts which are a basal medium and serum. The main source of the serum is come from an animal blood. The serum from animal blood has many disadvantages such as viral contaminations. Moreover, serum is very expensive. Serum-free media is the best alternative in solving this problem. Unfortunately, serum-free medium is not sufficient for the cell growth. Thus, some supplements must be added to serum-free medium. One of the most important sources of supplements is from plants which could reduce the cost. In this study different plant protein hydrolysates from soy, sesame, Aloe vera, rice and wheat have been tested against Human Skin Fibroblast (HSF) 1184. All protein hydrolysates were prepared through enzymatic hydrolysis using commercial enzymes of non-animal origin. These hydrolysates were characterized according to their solubility and peptide size. Different growth behaviours of HSF 1184 cells were observed when these hydrolysates were added in DMEM with and without Fetal Bovine Serum (FBS). Hydrolysates from exopeptidase enzymes such as Flavourzyme gave negative effect on HSF 1184 cell culture, while hydrolysates from endopeptidase enzymes were supplementary for HSF 1184 cell culture. Since plant proteins do not have all the necessary amino acids for HSF 1184 cell culture growth, they cannot be solely substituted with FBS. Depending on the enzyme used, the supplementation with hydrolysates corresponding to a high degree of hydrolysis and composition of peptides with small molecular size, led to different maximal cell density. This indicates the importance of enzyme specificity and consequently the nature of the released peptides. In conclusion, the best plant protein hydrolysates for supplementation into the complete medium for HSF 1184 growth were soy and Aloe vera which were hydrolysed by Alcalase. 2012 Thesis http://eprints.utm.my/id/eprint/32433/ http://eprints.utm.my/id/eprint/32433/1/AmirAbbasKazemzadehFarizhandiMFCE2012.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:70143?site_name=Restricted Repository masters Universiti Teknologi Malaysia, Faculty of Chemical Engineering dan Kejuruteraan Sumber Asli Faculty of Chemical Engineering dan Kejuruteraan Sumber Asli
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	Q Science (General)
spellingShingle	Q Science (General) Kazemzadeh Farizhandi, Amir Abbas Plant protein hydrolysates as a supplement for medium of human skin fibroblast 1184 cell culture
description	Medium is the most important item in cell culture. Each medium consists of two main parts which are a basal medium and serum. The main source of the serum is come from an animal blood. The serum from animal blood has many disadvantages such as viral contaminations. Moreover, serum is very expensive. Serum-free media is the best alternative in solving this problem. Unfortunately, serum-free medium is not sufficient for the cell growth. Thus, some supplements must be added to serum-free medium. One of the most important sources of supplements is from plants which could reduce the cost. In this study different plant protein hydrolysates from soy, sesame, Aloe vera, rice and wheat have been tested against Human Skin Fibroblast (HSF) 1184. All protein hydrolysates were prepared through enzymatic hydrolysis using commercial enzymes of non-animal origin. These hydrolysates were characterized according to their solubility and peptide size. Different growth behaviours of HSF 1184 cells were observed when these hydrolysates were added in DMEM with and without Fetal Bovine Serum (FBS). Hydrolysates from exopeptidase enzymes such as Flavourzyme gave negative effect on HSF 1184 cell culture, while hydrolysates from endopeptidase enzymes were supplementary for HSF 1184 cell culture. Since plant proteins do not have all the necessary amino acids for HSF 1184 cell culture growth, they cannot be solely substituted with FBS. Depending on the enzyme used, the supplementation with hydrolysates corresponding to a high degree of hydrolysis and composition of peptides with small molecular size, led to different maximal cell density. This indicates the importance of enzyme specificity and consequently the nature of the released peptides. In conclusion, the best plant protein hydrolysates for supplementation into the complete medium for HSF 1184 growth were soy and Aloe vera which were hydrolysed by Alcalase.
format	Thesis
qualification_level	Master's degree
author	Kazemzadeh Farizhandi, Amir Abbas
author_facet	Kazemzadeh Farizhandi, Amir Abbas
author_sort	Kazemzadeh Farizhandi, Amir Abbas
title	Plant protein hydrolysates as a supplement for medium of human skin fibroblast 1184 cell culture
title_short	Plant protein hydrolysates as a supplement for medium of human skin fibroblast 1184 cell culture
title_full	Plant protein hydrolysates as a supplement for medium of human skin fibroblast 1184 cell culture
title_fullStr	Plant protein hydrolysates as a supplement for medium of human skin fibroblast 1184 cell culture
title_full_unstemmed	Plant protein hydrolysates as a supplement for medium of human skin fibroblast 1184 cell culture
title_sort	plant protein hydrolysates as a supplement for medium of human skin fibroblast 1184 cell culture
granting_institution	Universiti Teknologi Malaysia, Faculty of Chemical Engineering dan Kejuruteraan Sumber Asli
granting_department	Faculty of Chemical Engineering dan Kejuruteraan Sumber Asli
publishDate	2012
url	http://eprints.utm.my/id/eprint/32433/1/AmirAbbasKazemzadehFarizhandiMFCE2012.pdf
_version_	1747816001334935552

Plant protein hydrolysates as a supplement for medium of human skin fibroblast 1184 cell culture

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