Silver inhibition on recombinant flavin reductase from Citrobacter freundii A1

Silver inhibition on recombinant flavin reductase from Citrobacter freundii A1

In this study, the flavin reductase (Fre) of Citrobatter freundii A1 was expressed in E. coli DH5a host with 1 mM IPTG induction. This recombinant protein was fused to 6xHis-tag, thus promising easier IMAC purification of the expressed enzyme. Flavin reductase catalyzes the reduction of free flavin...

Full description

Saved in:
Bibliographic Details
Main Author: Mamat, Nor Sasha Izana
Format: Thesis
Language:English
Published: 2012
Subjects:
TP Chemical technology
Online Access:http://eprints.utm.my/id/eprint/32976/5/NorSashaIzanaMamatMFBB2012.pdf
Tags: Add Tag
No Tags, Be the first to tag this record!
id my-utm-ep.32976
record_format uketd_dc
spelling my-utm-ep.329762018-04-30T13:09:48Z Silver inhibition on recombinant flavin reductase from Citrobacter freundii A1 2012-02 Mamat, Nor Sasha Izana TP Chemical technology In this study, the flavin reductase (Fre) of Citrobatter freundii A1 was expressed in E. coli DH5a host with 1 mM IPTG induction. This recombinant protein was fused to 6xHis-tag, thus promising easier IMAC purification of the expressed enzyme. Flavin reductase catalyzes the reduction of free flavin using NADH to produce free reduced flavin. The purity of recombinant flavin reductase was observed using 15 % SDS-PAGE and the molecular weight of the target protein was determined to be 27.04 kDa. In this study, flavin reductase activity was measured at 340 nm (e340 = 6.22 mM-1cm-1) due to the oxidation of NADH. The effect of silver ions from silver nitrate (AgNO3) on the activity of flavin reductase was determined. As the concentration of silver ions increased, the relative activity and velocity of flavin reductase reaction decreased over time. Silver ions may inhibit flavin reductase irreversibly via unspecific binding to different amino acids. In conclusion, silver ions could be a potent inhibitor of flavin reductase of C. freundii A1. 2012-02 Thesis http://eprints.utm.my/id/eprint/32976/ http://eprints.utm.my/id/eprint/32976/5/NorSashaIzanaMamatMFBB2012.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Biosciences and Bioengineering Faculty of Biosciences and Bioengineering
institution Universiti Teknologi Malaysia
collection UTM Institutional Repository
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Mamat, Nor Sasha Izana
Silver inhibition on recombinant flavin reductase from Citrobacter freundii A1
description In this study, the flavin reductase (Fre) of Citrobatter freundii A1 was expressed in E. coli DH5a host with 1 mM IPTG induction. This recombinant protein was fused to 6xHis-tag, thus promising easier IMAC purification of the expressed enzyme. Flavin reductase catalyzes the reduction of free flavin using NADH to produce free reduced flavin. The purity of recombinant flavin reductase was observed using 15 % SDS-PAGE and the molecular weight of the target protein was determined to be 27.04 kDa. In this study, flavin reductase activity was measured at 340 nm (e340 = 6.22 mM-1cm-1) due to the oxidation of NADH. The effect of silver ions from silver nitrate (AgNO3) on the activity of flavin reductase was determined. As the concentration of silver ions increased, the relative activity and velocity of flavin reductase reaction decreased over time. Silver ions may inhibit flavin reductase irreversibly via unspecific binding to different amino acids. In conclusion, silver ions could be a potent inhibitor of flavin reductase of C. freundii A1.
format Thesis
qualification_level Master's degree
author Mamat, Nor Sasha Izana
author_facet Mamat, Nor Sasha Izana
author_sort Mamat, Nor Sasha Izana
title Silver inhibition on recombinant flavin reductase from Citrobacter freundii A1
title_short Silver inhibition on recombinant flavin reductase from Citrobacter freundii A1
title_full Silver inhibition on recombinant flavin reductase from Citrobacter freundii A1
title_fullStr Silver inhibition on recombinant flavin reductase from Citrobacter freundii A1
title_full_unstemmed Silver inhibition on recombinant flavin reductase from Citrobacter freundii A1
title_sort silver inhibition on recombinant flavin reductase from citrobacter freundii a1
granting_institution Universiti Teknologi Malaysia, Faculty of Biosciences and Bioengineering
granting_department Faculty of Biosciences and Bioengineering
publishDate 2012
url http://eprints.utm.my/id/eprint/32976/5/NorSashaIzanaMamatMFBB2012.pdf
_version_ 1747816071326334976

Similar Items