Chemical constituents and antimicroblal activity of two arieties of Etlingera Elatior flowers

Etlingera elatior is synonymous with Phaeomeria speciosa, Alpinia elatior, Elettaria speciosa, Nicolaia elatior and Nicolaia speciosa. The species is native to Peninsular Malaysia and Indonesia. In Malaysia, it is called kantan. The essential oil and phytochemicals of two Etlingera elatior flowers v...

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Main Author:	Muhamad Azhar, Nurul Iryani
Format:	Thesis
Language:	English
Published:	2013
Subjects:	QK Botany
Online Access:	http://eprints.utm.my/id/eprint/33097/1/NurulIryaniMuhamadAzharMFS2013.pdf
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spelling	my-utm-ep.330972017-09-20T00:17:55Z Chemical constituents and antimicroblal activity of two arieties of Etlingera Elatior flowers 2013 Muhamad Azhar, Nurul Iryani QK Botany Etlingera elatior is synonymous with Phaeomeria speciosa, Alpinia elatior, Elettaria speciosa, Nicolaia elatior and Nicolaia speciosa. The species is native to Peninsular Malaysia and Indonesia. In Malaysia, it is called kantan. The essential oil and phytochemicals of two Etlingera elatior flowers varieties (pink and red flower variety) have been studied. The essential oil of the fresh E. elatior flowers was extracted using the hydrodistillation technique and analysed by Kovats Indices and Gas Chromatography-Mass Spectrometry (GC-MS). Hydrodistillation of E. elatior (pink flower variety) flowers gave 70.63% of essential oil classified as monoterpenes, sesquiterpenes, and oxygenated of monoterpenes and sesquiterpenes derivatives. The major constituent identified in the essential oil is cyclodecane (38.07%). Extraction of the dried flowers was done by soxhlet and cold extraction methods using chloroform and dichloromethane, respectively. Fractionation and purification on the crude extracts using Vacuum Liquid Chromatography (VLC) and Column Chromatography (CC) was successfully isolated ß-sitosterol and a mixture of stigmasterol and ß-sitosterol. Their structures were elucidated by GC, GC-MS, IR, 1H NMR and 13C NMR spectroscopies. The antimicrobial activity of essential oil, crude extracts and ß-sitosterol was carried out using disc diffusion method, Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC) assay with eight strains of bacteria, Enterococcus faecalis, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus (Gram positive), Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia and Pseudomonas putida (Gram negative). Antimicrobial screening showed that the essential oil gave moderate to strong inhibition to most of the bacteria with minimum inhibition concentration between 225-900 µg/mL. 2013 Thesis http://eprints.utm.my/id/eprint/33097/ http://eprints.utm.my/id/eprint/33097/1/NurulIryaniMuhamadAzharMFS2013.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:69177?site_name=Restricted Repository masters Universiti Teknologi Malaysia, Faculty of Science Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QK Botany
spellingShingle	QK Botany Muhamad Azhar, Nurul Iryani Chemical constituents and antimicroblal activity of two arieties of Etlingera Elatior flowers
description	Etlingera elatior is synonymous with Phaeomeria speciosa, Alpinia elatior, Elettaria speciosa, Nicolaia elatior and Nicolaia speciosa. The species is native to Peninsular Malaysia and Indonesia. In Malaysia, it is called kantan. The essential oil and phytochemicals of two Etlingera elatior flowers varieties (pink and red flower variety) have been studied. The essential oil of the fresh E. elatior flowers was extracted using the hydrodistillation technique and analysed by Kovats Indices and Gas Chromatography-Mass Spectrometry (GC-MS). Hydrodistillation of E. elatior (pink flower variety) flowers gave 70.63% of essential oil classified as monoterpenes, sesquiterpenes, and oxygenated of monoterpenes and sesquiterpenes derivatives. The major constituent identified in the essential oil is cyclodecane (38.07%). Extraction of the dried flowers was done by soxhlet and cold extraction methods using chloroform and dichloromethane, respectively. Fractionation and purification on the crude extracts using Vacuum Liquid Chromatography (VLC) and Column Chromatography (CC) was successfully isolated ß-sitosterol and a mixture of stigmasterol and ß-sitosterol. Their structures were elucidated by GC, GC-MS, IR, 1H NMR and 13C NMR spectroscopies. The antimicrobial activity of essential oil, crude extracts and ß-sitosterol was carried out using disc diffusion method, Minimum Inhibition Concentration (MIC) and Minimum Bactericidal Concentration (MBC) assay with eight strains of bacteria, Enterococcus faecalis, Staphylococcus aureus, Bacillus subtilis, Bacillus cereus (Gram positive), Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia and Pseudomonas putida (Gram negative). Antimicrobial screening showed that the essential oil gave moderate to strong inhibition to most of the bacteria with minimum inhibition concentration between 225-900 µg/mL.
format	Thesis
qualification_level	Master's degree
author	Muhamad Azhar, Nurul Iryani
author_facet	Muhamad Azhar, Nurul Iryani
author_sort	Muhamad Azhar, Nurul Iryani
title	Chemical constituents and antimicroblal activity of two arieties of Etlingera Elatior flowers
title_short	Chemical constituents and antimicroblal activity of two arieties of Etlingera Elatior flowers
title_full	Chemical constituents and antimicroblal activity of two arieties of Etlingera Elatior flowers
title_fullStr	Chemical constituents and antimicroblal activity of two arieties of Etlingera Elatior flowers
title_full_unstemmed	Chemical constituents and antimicroblal activity of two arieties of Etlingera Elatior flowers
title_sort	chemical constituents and antimicroblal activity of two arieties of etlingera elatior flowers
granting_institution	Universiti Teknologi Malaysia, Faculty of Science
granting_department	Faculty of Science
publishDate	2013
url	http://eprints.utm.my/id/eprint/33097/1/NurulIryaniMuhamadAzharMFS2013.pdf
_version_	1747816078640152576

Chemical constituents and antimicroblal activity of two arieties of Etlingera Elatior flowers

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