Chloroplast DNA diversification of Malaysian Pineapple

Chloroplast DNA diversification of Malaysian Pineapple

There are several genes are normally used for the phylogenetic study such as Mitochondria DNA (mtDNA), Chloroplast DNA (cpDNA) and Nuclear DNA (nDNA). Among these three genomes, cpDNA is commonly being used in phylogenetic study because it is easy to amplify via PCR, evolves at a conservation rate a...

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Main Author: Hamdan, Norfadilah
Format: Thesis
Language:English
Published: 2013
Subjects:
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Online Access:http://eprints.utm.my/id/eprint/33099/1/NorfadilahHamdanFBME2013.pdf
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spelling my-utm-ep.330992017-06-14T00:53:37Z Chloroplast DNA diversification of Malaysian Pineapple 2013 Hamdan, Norfadilah Unspecified There are several genes are normally used for the phylogenetic study such as Mitochondria DNA (mtDNA), Chloroplast DNA (cpDNA) and Nuclear DNA (nDNA). Among these three genomes, cpDNA is commonly being used in phylogenetic study because it is easy to amplify via PCR, evolves at a conservation rate and it has appropriate length and base substitution rate for inferring phylogeny at higher levels. In this study, phylogenetic tree of eight Malaysian pineapple (Ananas comosus) cultivars were constructed using sequences of large subunit of the ribulose- bisphosphate carboxylase (rbcL) gene. A rbcL gene was isolated from genomic DNA, amplified and sequenced. Phylogenetic analysis was carried out using maximum parsimony method. Results revealed that rbcL gene of Ananas comosus is about 1250 bps. Based on the tree, eight Malaysian pineapple cultivars were classified into two groups. The first group consisted of Yankee and Gandul cultivars while second group consisted of Moris, Moris Bentanggur, Moris Gajah, N36, Josaphine and Sarawak. Bootstrap value in some branches were low this is reflected by the small number of informative characters (981 were conserved and 85 were potentially informative) to build the tree. Formation of several group or subclades is due to its similar genetic pattern, thus support this system classification. The study suggested that rbcL gene could be used to determine the phylogenetic relationship distinguish the pineapple cultivars 2013 Thesis http://eprints.utm.my/id/eprint/33099/ http://eprints.utm.my/id/eprint/33099/1/NorfadilahHamdanFBME2013.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:79527?queryType=vitalDismax&query=Chloroplast+DNA+diversification+of+Malaysian+Pineapple&public=true masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution Universiti Teknologi Malaysia
collection UTM Institutional Repository
language English
topic Unspecified
spellingShingle Unspecified
Hamdan, Norfadilah
Chloroplast DNA diversification of Malaysian Pineapple
description There are several genes are normally used for the phylogenetic study such as Mitochondria DNA (mtDNA), Chloroplast DNA (cpDNA) and Nuclear DNA (nDNA). Among these three genomes, cpDNA is commonly being used in phylogenetic study because it is easy to amplify via PCR, evolves at a conservation rate and it has appropriate length and base substitution rate for inferring phylogeny at higher levels. In this study, phylogenetic tree of eight Malaysian pineapple (Ananas comosus) cultivars were constructed using sequences of large subunit of the ribulose- bisphosphate carboxylase (rbcL) gene. A rbcL gene was isolated from genomic DNA, amplified and sequenced. Phylogenetic analysis was carried out using maximum parsimony method. Results revealed that rbcL gene of Ananas comosus is about 1250 bps. Based on the tree, eight Malaysian pineapple cultivars were classified into two groups. The first group consisted of Yankee and Gandul cultivars while second group consisted of Moris, Moris Bentanggur, Moris Gajah, N36, Josaphine and Sarawak. Bootstrap value in some branches were low this is reflected by the small number of informative characters (981 were conserved and 85 were potentially informative) to build the tree. Formation of several group or subclades is due to its similar genetic pattern, thus support this system classification. The study suggested that rbcL gene could be used to determine the phylogenetic relationship distinguish the pineapple cultivars
format Thesis
qualification_level Master's degree
author Hamdan, Norfadilah
author_facet Hamdan, Norfadilah
author_sort Hamdan, Norfadilah
title Chloroplast DNA diversification of Malaysian Pineapple
title_short Chloroplast DNA diversification of Malaysian Pineapple
title_full Chloroplast DNA diversification of Malaysian Pineapple
title_fullStr Chloroplast DNA diversification of Malaysian Pineapple
title_full_unstemmed Chloroplast DNA diversification of Malaysian Pineapple
title_sort chloroplast dna diversification of malaysian pineapple
granting_institution Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department Faculty of Biosciences and Medical Engineering
publishDate 2013
url http://eprints.utm.my/id/eprint/33099/1/NorfadilahHamdanFBME2013.pdf
_version_ 1747816079126691840

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