The effect of preservative and temperature on blood alcohol concentration during storage period

Determination of blood alcohol concentration (BAC) on ante mortem and post mortem samples is often performed in forensic laboratory. BAC determination has been common task in forensic laboratory due to rising accident cases due to driving under influence of alcohol. Earlier researchers have proven t...

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Main Author:	Sinnathurai, Jayasilan
Format:	Thesis
Language:	English
Published:	2013
Subjects:	RA1001 Forensic Medicine Medical jurisprudence Legal medicine
Online Access:	http://eprints.utm.my/id/eprint/33139/1/JayasilanSinnathuraiMFS2013.pdf
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id	my-utm-ep.33139
record_format	uketd_dc
spelling	my-utm-ep.331392017-09-18T01:41:19Z The effect of preservative and temperature on blood alcohol concentration during storage period 2013-01 Sinnathurai, Jayasilan RA1001 Forensic Medicine. Medical jurisprudence. Legal medicine Determination of blood alcohol concentration (BAC) on ante mortem and post mortem samples is often performed in forensic laboratory. BAC determination has been common task in forensic laboratory due to rising accident cases due to driving under influence of alcohol. Earlier researchers have proven that factors such as preservative, storage temperature and storage period affect the blood alcohol stability and BAC determination. Apparently, no regulation governs these factors in BAC determination. Therefore, this study is performed to address the effect of preservative, temperature and storage days on BAC level. A blood specimen of ante and post mortem samples with preservative and non preservative in two different conditions (chilled temperature, 4-7oC and room temperature, 24-26.9oC) has been examined. The analysis was carried out on 1st day, 7th day and 14th day of blood sample received. The blood samples have been diluted with an aqueous internal standard solution of n-propanol and analyzed using gas chromatography with flame ionization detection (GC-FID). The analysis showed that the temperature and preservative have no effect on BAC of ante mortem and post mortem blood samples and blood alcohol analysis can be performed within 21 days. 2013-01 Thesis http://eprints.utm.my/id/eprint/33139/ http://eprints.utm.my/id/eprint/33139/1/JayasilanSinnathuraiMFS2013.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Science Faculty of Science
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	RA1001 Forensic Medicine Medical jurisprudence Legal medicine
spellingShingle	RA1001 Forensic Medicine Medical jurisprudence Legal medicine Sinnathurai, Jayasilan The effect of preservative and temperature on blood alcohol concentration during storage period
description	Determination of blood alcohol concentration (BAC) on ante mortem and post mortem samples is often performed in forensic laboratory. BAC determination has been common task in forensic laboratory due to rising accident cases due to driving under influence of alcohol. Earlier researchers have proven that factors such as preservative, storage temperature and storage period affect the blood alcohol stability and BAC determination. Apparently, no regulation governs these factors in BAC determination. Therefore, this study is performed to address the effect of preservative, temperature and storage days on BAC level. A blood specimen of ante and post mortem samples with preservative and non preservative in two different conditions (chilled temperature, 4-7oC and room temperature, 24-26.9oC) has been examined. The analysis was carried out on 1st day, 7th day and 14th day of blood sample received. The blood samples have been diluted with an aqueous internal standard solution of n-propanol and analyzed using gas chromatography with flame ionization detection (GC-FID). The analysis showed that the temperature and preservative have no effect on BAC of ante mortem and post mortem blood samples and blood alcohol analysis can be performed within 21 days.
format	Thesis
qualification_level	Master's degree
author	Sinnathurai, Jayasilan
author_facet	Sinnathurai, Jayasilan
author_sort	Sinnathurai, Jayasilan
title	The effect of preservative and temperature on blood alcohol concentration during storage period
title_short	The effect of preservative and temperature on blood alcohol concentration during storage period
title_full	The effect of preservative and temperature on blood alcohol concentration during storage period
title_fullStr	The effect of preservative and temperature on blood alcohol concentration during storage period
title_full_unstemmed	The effect of preservative and temperature on blood alcohol concentration during storage period
title_sort	effect of preservative and temperature on blood alcohol concentration during storage period
granting_institution	Universiti Teknologi Malaysia, Faculty of Science
granting_department	Faculty of Science
publishDate	2013
url	http://eprints.utm.my/id/eprint/33139/1/JayasilanSinnathuraiMFS2013.pdf
_version_	1747816088895225856

The effect of preservative and temperature on blood alcohol concentration during storage period

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