Expression of ron tyrosin kinase receptor on breast cancer cell lines

RON receptor is one member of the MET family of receptor tyrosine kinases. When it is activated by its own ligand, macrophage stimulating protein (MSP), RON initiates a downstream signaling pathways which result of some function in the cell such as dissociation, migration, and invasion. Several RON...

Full description

Saved in:

Bibliographic Details
Main Author:	Alzeini, Khaled W. K.
Format:	Thesis
Language:	English
Published:	2013
Subjects:	Unspecified
Online Access:	http://eprints.utm.my/id/eprint/33162/1/KhaledWKAlzeiniMFBME2013.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-utm-ep.33162
record_format	uketd_dc
spelling	my-utm-ep.331622017-06-20T00:50:21Z Expression of ron tyrosin kinase receptor on breast cancer cell lines 2013 Alzeini, Khaled W. K. Unspecified RON receptor is one member of the MET family of receptor tyrosine kinases. When it is activated by its own ligand, macrophage stimulating protein (MSP), RON initiates a downstream signaling pathways which result of some function in the cell such as dissociation, migration, and invasion. Several RON variants that arise through mRNA splicing or by an alternate translational start site have been identified, some with oncogenic potential. The purpose of the present project was to determine the expression of RON receptor in two breast cancer cell lines since it is the most common distribution cancer in women around the world. MDA-MB-213 and MCF-7 breast cancer cell lines and WRL-68 embryonic liver as a normal cell line were used in this experiment to characterize the expression. The expression of RON receptor on the cell surface, the concentration of mRNA and the RON protein were done by utilizing Immunofluorescent staining (IF), polymerase chain reaction (PCR) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) respectively. Collectively, it was found that RON receptor was overexpressed in these two breast cancer cell lines comparing to the normal cell lines. 2013 Thesis http://eprints.utm.my/id/eprint/33162/ http://eprints.utm.my/id/eprint/33162/1/KhaledWKAlzeiniMFBME2013.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:69994?queryType=vitalDismax&query=Expression+of+ron+tyrosin+kinase+receptor+on+breast+cancer+cell+lines&public=true masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	Unspecified
spellingShingle	Unspecified Alzeini, Khaled W. K. Expression of ron tyrosin kinase receptor on breast cancer cell lines
description	RON receptor is one member of the MET family of receptor tyrosine kinases. When it is activated by its own ligand, macrophage stimulating protein (MSP), RON initiates a downstream signaling pathways which result of some function in the cell such as dissociation, migration, and invasion. Several RON variants that arise through mRNA splicing or by an alternate translational start site have been identified, some with oncogenic potential. The purpose of the present project was to determine the expression of RON receptor in two breast cancer cell lines since it is the most common distribution cancer in women around the world. MDA-MB-213 and MCF-7 breast cancer cell lines and WRL-68 embryonic liver as a normal cell line were used in this experiment to characterize the expression. The expression of RON receptor on the cell surface, the concentration of mRNA and the RON protein were done by utilizing Immunofluorescent staining (IF), polymerase chain reaction (PCR) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) respectively. Collectively, it was found that RON receptor was overexpressed in these two breast cancer cell lines comparing to the normal cell lines.
format	Thesis
qualification_level	Master's degree
author	Alzeini, Khaled W. K.
author_facet	Alzeini, Khaled W. K.
author_sort	Alzeini, Khaled W. K.
title	Expression of ron tyrosin kinase receptor on breast cancer cell lines
title_short	Expression of ron tyrosin kinase receptor on breast cancer cell lines
title_full	Expression of ron tyrosin kinase receptor on breast cancer cell lines
title_fullStr	Expression of ron tyrosin kinase receptor on breast cancer cell lines
title_full_unstemmed	Expression of ron tyrosin kinase receptor on breast cancer cell lines
title_sort	expression of ron tyrosin kinase receptor on breast cancer cell lines
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2013
url	http://eprints.utm.my/id/eprint/33162/1/KhaledWKAlzeiniMFBME2013.pdf
_version_	1747816094505107456

Expression of ron tyrosin kinase receptor on breast cancer cell lines

Similar Items