High cell density cultivation on Hendersonia sp. for the application of biological control

Malaysia is one the world’s biggest producers and exporters of oil palm products. In 2011, Malaysia has exported 24.27 million tonnes of oil palm products, which accounted for RM 80.4 billion in total export revenue. However, the oil palm is susceptible to plant diseases, especially Basal Stem Rot c...

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Main Author:	Azman, Muhammad Danial
Format:	Thesis
Language:	English
Published:	2013
Subjects:	SB Plant culture
Online Access:	http://eprints.utm.my/id/eprint/33187/5/MuhammadDanialAzmanMFKK2013.pdf
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spelling	my-utm-ep.331872017-09-11T00:36:54Z High cell density cultivation on Hendersonia sp. for the application of biological control 2013-01 Azman, Muhammad Danial SB Plant culture Malaysia is one the world’s biggest producers and exporters of oil palm products. In 2011, Malaysia has exported 24.27 million tonnes of oil palm products, which accounted for RM 80.4 billion in total export revenue. However, the oil palm is susceptible to plant diseases, especially Basal Stem Rot caused by Ganoderma. This disease has caused tremendous losses to the economy. Hendersonia sp. is a novel fungus strain that has shown effective results in controlling Ganoderma infection. The aim of this study was to establish a comprehensive Hendersonia cultivation platform for industrial-scale production. In an agar medium screening study, the best medium was composed of (g/L): Malt Extract, 20; Glucose, 20; Peptone,1; Yeast Extract, 5; Agar powder, 20. Based from the shake flask media screening study, the medium composed of (g/L): Malt extract, 20; Glucose, 20; Peptone, 1; Yeast extract, 5, gave the highest CDW of 7.45±0.6 g/L. This is followed by the medium composed of (g/L): Sucrose, 40; KH2PO4, 1; MgSO4.7H2O, 1.0; KCl, 0.5; FeSO4, 0.01; Yeast Extract, 2, which resulted with CDW of 6.15±0.14 g/L. The optimization of the medium was applied by using Response Surface Methodology. The new optimized medium formulation was composed of (g/L): Sucrose, 60; K2HPO4, 0.5; Yeast Extract, 3; MgSO4.7H2O, 1; KCl, 0.5; FeSO4, 0.01. For the 150-L bioreactor cultivation, by utilizing un-controlled pH throughout the cultivation, 13.55 g/L of CDW was obtained. In addition, the effects of agitation speed on the cell mass during the 150-L bioreactor cultivation were studied. The highest CDW (15.6 g/L) was obtained with agitation speed of 250 rpm at 70 h. In conclusion, the present study has proposed a reliable and cost-effective approach in mass producing Hendersonia in industrial scale. 2013-01 Thesis http://eprints.utm.my/id/eprint/33187/ http://eprints.utm.my/id/eprint/33187/5/MuhammadDanialAzmanMFKK2013.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:70123?site_name=Restricted Repository masters Universiti Teknologi Malaysia, Faculty of Chemical Engineering Faculty of Chemical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	SB Plant culture
spellingShingle	SB Plant culture Azman, Muhammad Danial High cell density cultivation on Hendersonia sp. for the application of biological control
description	Malaysia is one the world’s biggest producers and exporters of oil palm products. In 2011, Malaysia has exported 24.27 million tonnes of oil palm products, which accounted for RM 80.4 billion in total export revenue. However, the oil palm is susceptible to plant diseases, especially Basal Stem Rot caused by Ganoderma. This disease has caused tremendous losses to the economy. Hendersonia sp. is a novel fungus strain that has shown effective results in controlling Ganoderma infection. The aim of this study was to establish a comprehensive Hendersonia cultivation platform for industrial-scale production. In an agar medium screening study, the best medium was composed of (g/L): Malt Extract, 20; Glucose, 20; Peptone,1; Yeast Extract, 5; Agar powder, 20. Based from the shake flask media screening study, the medium composed of (g/L): Malt extract, 20; Glucose, 20; Peptone, 1; Yeast extract, 5, gave the highest CDW of 7.45±0.6 g/L. This is followed by the medium composed of (g/L): Sucrose, 40; KH2PO4, 1; MgSO4.7H2O, 1.0; KCl, 0.5; FeSO4, 0.01; Yeast Extract, 2, which resulted with CDW of 6.15±0.14 g/L. The optimization of the medium was applied by using Response Surface Methodology. The new optimized medium formulation was composed of (g/L): Sucrose, 60; K2HPO4, 0.5; Yeast Extract, 3; MgSO4.7H2O, 1; KCl, 0.5; FeSO4, 0.01. For the 150-L bioreactor cultivation, by utilizing un-controlled pH throughout the cultivation, 13.55 g/L of CDW was obtained. In addition, the effects of agitation speed on the cell mass during the 150-L bioreactor cultivation were studied. The highest CDW (15.6 g/L) was obtained with agitation speed of 250 rpm at 70 h. In conclusion, the present study has proposed a reliable and cost-effective approach in mass producing Hendersonia in industrial scale.
format	Thesis
qualification_level	Master's degree
author	Azman, Muhammad Danial
author_facet	Azman, Muhammad Danial
author_sort	Azman, Muhammad Danial
title	High cell density cultivation on Hendersonia sp. for the application of biological control
title_short	High cell density cultivation on Hendersonia sp. for the application of biological control
title_full	High cell density cultivation on Hendersonia sp. for the application of biological control
title_fullStr	High cell density cultivation on Hendersonia sp. for the application of biological control
title_full_unstemmed	High cell density cultivation on Hendersonia sp. for the application of biological control
title_sort	high cell density cultivation on hendersonia sp. for the application of biological control
granting_institution	Universiti Teknologi Malaysia, Faculty of Chemical Engineering
granting_department	Faculty of Chemical Engineering
publishDate	2013
url	http://eprints.utm.my/id/eprint/33187/5/MuhammadDanialAzmanMFKK2013.pdf
_version_	1747816100380278784

High cell density cultivation on Hendersonia sp. for the application of biological control

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