Isolation and identification of bacteria capable of sulfate reduction from palm oil sludge

Sulfur in its native is a yellow crystalline solid. In nature, it occurs as the pure element or as sulfide and sulfate minerals. Inorganic sulfur compounds can be found in the form of sulfate, sulfide, sulfite, thiosulfate, elemental sulfur and polythionates. Sulfate appears to be the most stable an...

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Main Author:	Mirfasih, Noosha
Format:	Thesis
Language:	English
Published:	2013
Subjects:	TD Environmental technology Sanitary engineering
Online Access:	http://eprints.utm.my/id/eprint/33209/1/NooshaMirfasihMFBB2013.pdf
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id	my-utm-ep.33209
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spelling	my-utm-ep.332092017-09-13T03:38:01Z Isolation and identification of bacteria capable of sulfate reduction from palm oil sludge 2013-01 Mirfasih, Noosha TD Environmental technology. Sanitary engineering Sulfur in its native is a yellow crystalline solid. In nature, it occurs as the pure element or as sulfide and sulfate minerals. Inorganic sulfur compounds can be found in the form of sulfate, sulfide, sulfite, thiosulfate, elemental sulfur and polythionates. Sulfate appears to be the most stable and abundant form of sulfur available for use by living organism in the biosphere. This present study focused on the isolation and identification of bacteria capable of sulfate reduction from palm oil sludge (POS). POS is one of the most difficult and complex industrial waste produced in Malaysia from palm oil processing plants. Three different samples of POS were collected from different pond of Palm Oil effluent in palm oil processing plant in Sedenak, Kulai, Malaysia. The concentration of sulfate content in the samples were analysed in order to determine the sample that contain high population of bacteria capable of sulfate reduction. This ensures the possibility of isolating the bacteria of interest from the selected sample. Results from SRB-Bart kit analysis showed that POS from raw pond contain high population of SRB or related bacteria and was chosen for further isolation of the bacteria. Isolation of the bacteria was conducted using selective enrichment method followed by growth on solid medium using rolling bottle technique. The isolation has successfully separated five different pure culture coded X2 , X1, E, B and C that were further identified using the analysis of amplified 16S rRNA sequences of the individual bacterium. Four of the bacteria namely E, B, X1, and X2 were found able to reductively degrade sulfate. These bacteria were able to grow and reduce limited amount of sulfate thus indicated to the assimilatory reduction of sulfate activity of these bacteria. 2013-01 Thesis http://eprints.utm.my/id/eprint/33209/ http://eprints.utm.my/id/eprint/33209/1/NooshaMirfasihMFBB2013.pdf application/pdf en public http://dms.library.utm.my:8080/vital/access/manager/Repository/vital:69821?site_name=Restricted Repository masters Universiti Teknologi Malaysia, Faculty of Bioscience and Bioengineering Faculty of Bioscience and Bioengineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	TD Environmental technology Sanitary engineering
spellingShingle	TD Environmental technology Sanitary engineering Mirfasih, Noosha Isolation and identification of bacteria capable of sulfate reduction from palm oil sludge
description	Sulfur in its native is a yellow crystalline solid. In nature, it occurs as the pure element or as sulfide and sulfate minerals. Inorganic sulfur compounds can be found in the form of sulfate, sulfide, sulfite, thiosulfate, elemental sulfur and polythionates. Sulfate appears to be the most stable and abundant form of sulfur available for use by living organism in the biosphere. This present study focused on the isolation and identification of bacteria capable of sulfate reduction from palm oil sludge (POS). POS is one of the most difficult and complex industrial waste produced in Malaysia from palm oil processing plants. Three different samples of POS were collected from different pond of Palm Oil effluent in palm oil processing plant in Sedenak, Kulai, Malaysia. The concentration of sulfate content in the samples were analysed in order to determine the sample that contain high population of bacteria capable of sulfate reduction. This ensures the possibility of isolating the bacteria of interest from the selected sample. Results from SRB-Bart kit analysis showed that POS from raw pond contain high population of SRB or related bacteria and was chosen for further isolation of the bacteria. Isolation of the bacteria was conducted using selective enrichment method followed by growth on solid medium using rolling bottle technique. The isolation has successfully separated five different pure culture coded X2 , X1, E, B and C that were further identified using the analysis of amplified 16S rRNA sequences of the individual bacterium. Four of the bacteria namely E, B, X1, and X2 were found able to reductively degrade sulfate. These bacteria were able to grow and reduce limited amount of sulfate thus indicated to the assimilatory reduction of sulfate activity of these bacteria.
format	Thesis
qualification_level	Master's degree
author	Mirfasih, Noosha
author_facet	Mirfasih, Noosha
author_sort	Mirfasih, Noosha
title	Isolation and identification of bacteria capable of sulfate reduction from palm oil sludge
title_short	Isolation and identification of bacteria capable of sulfate reduction from palm oil sludge
title_full	Isolation and identification of bacteria capable of sulfate reduction from palm oil sludge
title_fullStr	Isolation and identification of bacteria capable of sulfate reduction from palm oil sludge
title_full_unstemmed	Isolation and identification of bacteria capable of sulfate reduction from palm oil sludge
title_sort	isolation and identification of bacteria capable of sulfate reduction from palm oil sludge
granting_institution	Universiti Teknologi Malaysia, Faculty of Bioscience and Bioengineering
granting_department	Faculty of Bioscience and Bioengineering
publishDate	2013
url	http://eprints.utm.my/id/eprint/33209/1/NooshaMirfasihMFBB2013.pdf
_version_	1747816105768910848

Isolation and identification of bacteria capable of sulfate reduction from palm oil sludge

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