Preliminary study on porphyrin derivatives as transfection reagents for mammalian cell

Porphyrins are organic, aromatic compounds found in heme, cytochrome, cobalamin, chlorophyll and many other natural products with essential roles in biological processes that their cationic forms has been used as a groups of favorable non-viral vectors recently. Cationic porphyrins are self-chromoge...

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Main Author:	Khorami, Hajar Hossein
Format:	Thesis
Language:	English
Published:	2013
Subjects:	QP Physiology
Online Access:	http://eprints.utm.my/id/eprint/33254/5/HajarHosseinKhoramiMFBSK2013.pdf
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spelling	my-utm-ep.332542017-09-12T07:29:04Z Preliminary study on porphyrin derivatives as transfection reagents for mammalian cell 2013-01 Khorami, Hajar Hossein QP Physiology Porphyrins are organic, aromatic compounds found in heme, cytochrome, cobalamin, chlorophyll and many other natural products with essential roles in biological processes that their cationic forms has been used as a groups of favorable non-viral vectors recently. Cationic porphyrins are self-chromogenic reagents with high capacity for modifications, great interaction with DNA and protection of DNA from nuclease during delivery of it into cell with low toxicity. In order to have high efficient gene transfection into cell while causing low toxicity, genetically manipulations of nonviral vector, cationic porphyrin, would be useful. In this study newly modified cationic porphyrins namely, 5-hexyl-10,15,20tris (N-methyl-4-pyridyl) porphyrin, 5-propyl- 10,15,20tris (N-methyl-4-pyridyl) porphyrin, 5,10-dipropyl-15,20-bis (N-methyl-4- pyridyl) porphyrin, 5,10-dihexyl-15,20bis (N-methyl-4-pyridyl) porphyrin, and polyamidoamine (PAMAM) G4-porphyrin conjugate were applied. Cytotoxicity of synthesize cationic porphyrins on Chinese Hamster Ovarian (CHO) cells, were evaluated by using MTT assay. Generally, all cationic derivatives are dose dependent, with low cytotoxicity at the ranges from 100 µM to 0.01µM. Four of cationic porphyrin were uptake by cell at high concentration while none were observed on conjugate one. Using different concentration of cationic porphyrins and methods were tested on transfection of CHO cells by using the derived transfection reagent with X-tremeGENE HP DNA as positive control. However no transfection observed by all the porphyrin derivat ives and the parameters tested except for positive control. Results of this study suggested that applying different protocol, and also trying other concentration of cationic porphyrins and DNA for forming a strong complex would increase the possibility of efficient gene transfection by using cationic porphyrins. 2013-01 Thesis http://eprints.utm.my/id/eprint/33254/ http://eprints.utm.my/id/eprint/33254/5/HajarHosseinKhoramiMFBSK2013.pdf application/pdf en public masters Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering Faculty of Biosciences and Medical Engineering
institution	Universiti Teknologi Malaysia
collection	UTM Institutional Repository
language	English
topic	QP Physiology
spellingShingle	QP Physiology Khorami, Hajar Hossein Preliminary study on porphyrin derivatives as transfection reagents for mammalian cell
description	Porphyrins are organic, aromatic compounds found in heme, cytochrome, cobalamin, chlorophyll and many other natural products with essential roles in biological processes that their cationic forms has been used as a groups of favorable non-viral vectors recently. Cationic porphyrins are self-chromogenic reagents with high capacity for modifications, great interaction with DNA and protection of DNA from nuclease during delivery of it into cell with low toxicity. In order to have high efficient gene transfection into cell while causing low toxicity, genetically manipulations of nonviral vector, cationic porphyrin, would be useful. In this study newly modified cationic porphyrins namely, 5-hexyl-10,15,20tris (N-methyl-4-pyridyl) porphyrin, 5-propyl- 10,15,20tris (N-methyl-4-pyridyl) porphyrin, 5,10-dipropyl-15,20-bis (N-methyl-4- pyridyl) porphyrin, 5,10-dihexyl-15,20bis (N-methyl-4-pyridyl) porphyrin, and polyamidoamine (PAMAM) G4-porphyrin conjugate were applied. Cytotoxicity of synthesize cationic porphyrins on Chinese Hamster Ovarian (CHO) cells, were evaluated by using MTT assay. Generally, all cationic derivatives are dose dependent, with low cytotoxicity at the ranges from 100 µM to 0.01µM. Four of cationic porphyrin were uptake by cell at high concentration while none were observed on conjugate one. Using different concentration of cationic porphyrins and methods were tested on transfection of CHO cells by using the derived transfection reagent with X-tremeGENE HP DNA as positive control. However no transfection observed by all the porphyrin derivat ives and the parameters tested except for positive control. Results of this study suggested that applying different protocol, and also trying other concentration of cationic porphyrins and DNA for forming a strong complex would increase the possibility of efficient gene transfection by using cationic porphyrins.
format	Thesis
qualification_level	Master's degree
author	Khorami, Hajar Hossein
author_facet	Khorami, Hajar Hossein
author_sort	Khorami, Hajar Hossein
title	Preliminary study on porphyrin derivatives as transfection reagents for mammalian cell
title_short	Preliminary study on porphyrin derivatives as transfection reagents for mammalian cell
title_full	Preliminary study on porphyrin derivatives as transfection reagents for mammalian cell
title_fullStr	Preliminary study on porphyrin derivatives as transfection reagents for mammalian cell
title_full_unstemmed	Preliminary study on porphyrin derivatives as transfection reagents for mammalian cell
title_sort	preliminary study on porphyrin derivatives as transfection reagents for mammalian cell
granting_institution	Universiti Teknologi Malaysia, Faculty of Biosciences and Medical Engineering
granting_department	Faculty of Biosciences and Medical Engineering
publishDate	2013
url	http://eprints.utm.my/id/eprint/33254/5/HajarHosseinKhoramiMFBSK2013.pdf
_version_	1747816116829290496

Preliminary study on porphyrin derivatives as transfection reagents for mammalian cell

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